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Orexin R2/HCRTR2 Antibody

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Western Blot: Orexin R2/HCRTR2 Antibody [NB100-74579] - (1.5ug/ml) staining of Human Brain (Hippocampus) lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Product Details

Summary
Reactivity Hu, Po, Bv, CaSpecies Glossary
Applications WB, ELISA
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

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Orexin R2/HCRTR2 Antibody Summary

Immunogen
Peptide with sequence SGTKLEDSPPCRN corresponding to N-Terminus according to NP_001517.1.
Epitope
SGTKLEDSPPCRN
Localization
Isoform 1 and isoform 4: Cell membrane; single pass type I membrane protein. Isoform 2 and isoform 3: Secreted protein.
Predicted Species
Porcine (100%), Bovine (100%), Canine (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
HCRTR2
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Peptide ELISA Detection limit 1:32000
  • Western Blot 1 - 3 ug/ml
Application Notes
WB: Approx. 50 kDa band observed in human brain (cerebral cortex, hippocampus and substantia nigra) lysates (calculated MW of 50.7 kDa band according to NP_001517.1).
Publications
Read Publication using NB100-74579.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Orexin R2/HCRTR2 Antibody

  • HCRTR2
  • hypocretin (orexin) receptor 2
  • Hypocretin receptor type 2
  • Orexin R2
  • Orexin Receptor 2
  • orexin receptor type 2
  • OrexinR2
  • OX2R
  • Ox-2-R
  • ox2-R

Background

HCRTR2 is a G-protein coupled receptor expressed exclusively in the brain. It has 64% identity with HCRTR1. HCRTR2 binds both orexin A and orexin B neuropeptides. HCRTR2 is involved in the central feedback mechanism that regulates feeding behaviour.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Orexin R2/HCRTR2 Antibody (NB100-74579)(1)

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Product General Protocols

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Video Protocols

WB Video Protocol

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol HCRTR2
Entrez
Uniprot