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Orexin A/Hypocretin-1 Antibody (97505) [Unconjugated]

 (19 Publications)
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Orexin A/Hypocretin‑1 was detected in immersion fixed paraffin-embedded sections of Mouse Hypothalamus using Mouse Anti-Human/Mouse Orexin A/Hypocretin‑1 Monoclonal Antibody (Catalog # MAB763) at 5 µg/mL overnight at 4 °C. Tissue was stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to synaptic boutons. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.
Orexin A/Hypocretin‑1 was detected in immersion fixed paraffin-embedded sections of Mouse Hypothalamus using Mouse Anti-Human/Mouse Orexin A/Hypocretin‑1 Monoclonal Antibody (Catalog # MAB763) at ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications IHC
Clone
97505
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Biotin
BAM763
View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
MAB763-SP
Carrier Free
MAB763-500
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Orexin A/Hypocretin-1 Antibody (97505) [Unconjugated] Summary

Immunogen
KLH-coupled human Orexin A/Hypocretin‑1 synthetic peptide
Accession # O43612
Specificity
Detects human and mouse Orexin A/Hypocretin‑1. In dot blots, no cross-reactivity with recombinant human Orexin B is observed.
Source
N/A
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
HCRT
Purity Statement
Protein A or G purified from ascites
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Immunohistochemistry 5-25 ug/mL
Publications
Read Publications using
MAB763 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Orexin A/Hypocretin-1 Antibody (97505) [Unconjugated]

  • HCRT
  • Hcrt1
  • hypocretin (orexin) neuropeptide precursor
  • Hypocretin1
  • Hypocretin-1
  • NRCLP1
  • Orexin A
  • orexin
  • OXHcrt
  • PPORX
  • PPOXHypocretin

Background

Orexin A and Orexin B are neuropeptide hormones derived from proteolytic processing of the Orexin precursor protein. Orexins are released from the hypothalamus and are involved in the regulation of sleeping patterns, feeding, and metabolism. The synthetic peptide immunogen corresponds to amino acid 34 - 66 of the human Orexin precursor (accession #O43612) (1).
  1. Sakurai, T. et al. (1998) Cell 92:573.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Orexin
A/Hypocretin-1 Antibo ...
MAB763
Orexin A/Hypocretin-1 Antibody (97505) [Unconjugated]
Species: Hu, Mu
Applications: IHC

Publications for Orexin A/Hypocretin-1 Antibody (MAB763)(19)

We have publications tested in 5 confirmed species: Human, Mouse, Rat, Alpaca, Canine.

We have publications tested in 4 applications: ICC, IHC, IHC-Fr, IHC-P.


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ICC
(1)
IHC
(3)
IHC-Fr
(5)
IHC-P
(2)
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Human
(1)
Mouse
(6)
Rat
(2)
Alpaca
(1)
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Showing Publications 1 - 10 of 19. Show All 19 Publications.
Publications using MAB763 Applications Species
Soledad Bárez-López, André S. Mecawi, Natasha Bryan, Audrys G. Pauža, Victor J. Duque, Benjamin T. Gillard et al. Translational and post-translational dynamics in a model peptidergic system Molecular & Cellular Proteomics 5/1/2023 [PMID: 37030596]
Fang Zhou, Dan Wang, Huiming Li, Sa Wang, Xinxin Zhang, Ao Li et al. Orexinergic innervations at GABAergic neurons of the lateral habenula mediates the anesthetic potency of sevoflurane CNS Neuroscience & Therapeutics 5/1/2023 [PMID: 36740262]
John F. Smiley, Cynthia Bleiwas, Stefanie Canals-Baker, Sharifa Z. Williams, Robert Sears, Catia M. Teixeira et al. Neonatal ethanol causes profound reduction of cholinergic cell number in the basal forebrain of adult animals Alcohol 12/1/2021 [PMID: 34464696]
Sarah L. Reitz, Andrzej Z. Wasilczuk, Gretel H. Beh, Alex Proekt, Max B. Kelz Activation of Preoptic Tachykinin 1 Neurons Promotes Wakefulness over Sleep and Volatile Anesthetic-Induced Unconsciousness Current Biology 1/1/2021 [PMID: 33188746]
Daiqiang Liu, Jiayan Li, Jiayi Wu, Jiaqi Dai, Xinfeng Chen, Yujie Huang et al. Monochromatic Blue Light Activates Suprachiasmatic Nucleus Neuronal Activity and Promotes Arousal in Mice Under Sevoflurane Anesthesia Frontiers in Neural Circuits 8/18/2020 [PMID: 32973462]
Leslie R. Amodeo, Wen Liu, Derek N. Wills, Ryan P. Vetreno, Fulton T. Crews, Cindy L. Ehlers Adolescent alcohol exposure increases orexin-A/hypocretin-1 in the anterior hypothalamus Alcohol 11/1/2020 [PMID: 32619610]
Nirinjini Naidoo, Jingxu Zhu, Raymond J. Galante, Jie Lian, Ewa Strus, Amy Lee et al. Reduction of the molecular chaperone binding immunoglobulin protein (BiP) accentuates the effect of aging on sleep-wake behavior Neurobiology of Aging 9/1/2018 [PMID: 29843048]
K Ogawa, H Suga, C Ozone, M Sakakibara, T Yamada, M Kano, K Mitsumoto, T Kasai, Y Kodani, H Nagasaki, N Yamamoto, D Hagiwara, M Goto, R Banno, Y Sugimura, H Arima Vasopressin-secreting neurons derived from human embryonic stem cells through specific induction of dorsal hypothalamic progenitors Sci Rep, 2018-02-26;8(1):3615. 2018-02-26 [PMID: 29483626] (ICC, Human) ICC Human
G Liguori, C Squillacio, L Assisi, A Pelagalli, A Vittoria, A Costagliol, N Mirabella Potential role of orexin A binding the receptor 1 for orexins in normal and cryptorchid dogs BMC Vet. Res., 2018-02-27;14(1):55. 2018-02-27 [PMID: 29482574] (IHC, Canine) IHC Canine
G Liguori, C Squillacio, L Assisi, N Mirabella, E Langella, A Costagliol, A Vittoria Localization of orexin B and receptor 2 for orexins in testicular cytotypes of the camelid alpaca (Vicugna pacos) Reprod. Domest. Anim, 2017-02-08;0(0):. 2017-02-08 [PMID: 28181310] (IHC, Alpaca) IHC Alpaca
Show All 19 Publications.

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Bioinformatics

Gene Symbol HCRT
Uniprot