| Reactivity | HuSpecies Glossary |
| Applications | AC |
| Description | A recombinant protein antigen with a N-terminal His6-ABP tag corresponding to human NUP35. Source: E. coli Amino Acid Sequence: PPVRSIYDDISSPGLGSTPLTSRRQPNISVMQSPLVGVTSTPGTGQSMFSPASIGQPRKTTLSPAQLDP Fusion Tag: N-terminal His6ABP (ABP = Albumin Binding Protein derived from Streptococcal Protein G) This product is intended to be used as a blocking antigen for antibody competition assays. Any other use of this antigen is done at the risk of the user. The use of this product for commercial production is strictly prohibited. Please contact technical support if you have any questions. |
| Source | E. coli |
| Protein/Peptide Type | Recombinant Protein Antigen |
| Gene | NUP35 |
| Purity | >80% by SDS-PAGE and Coomassie blue staining |
| Dilutions |
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| Application Notes | This recombinant antigen is only intended to be used as a blocking agent to confirm antibody specificity with the corresponding antibody, catalog number NBP1-92213. It is purified by IMAC chromatography, and the expected concentration is greater than 0.5 mg/ml. For current lot information, including availability, please contact our technical support team click nb-technical@bio-techne.com |
| Theoretical MW | 25 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Store at -20C. Avoid freeze-thaw cycles. |
| Buffer | PBS and 1M Urea, pH 7.4. |
| Preservative | No Preservative |
| Purity | >80% by SDS-PAGE and Coomassie blue staining |
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| Gene Symbol | NUP35 |
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![Immunoprecipitation: Nup53 Antibody [NB100-93322] - Detection of human NUP35 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-NUP35 antibody NB100-93322 (lot NB100-93322-2) used for IP at 6 ug per reaction. NUP35 was also immunoprecipitated by a previous lot of this antibody (lot NB100-93322-1) and another rabbit anti-NUP35 antibody. For blotting immunoprecipitated NUP35, NB100-93322 was used at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](https://images.novusbio.com/images/Nup53-Antibody-Immunoprecipitation-NB100-93322-img0012.jpg "Immunoprecipitation: Nup53 Antibody [NB100-93322] - Detection of human NUP35 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-NUP35 antibody NB100-93322 (lot NB100-93322-2) used for IP at 6 ug per reaction. NUP35 was also immunoprecipitated by a previous lot of this antibody (lot NB100-93322-1) and another rabbit anti-NUP35 antibody. For blotting immunoprecipitated NUP35, NB100-93322 was used at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.")