Nup53 Antibodies
We offer Nup53 Antibodies for use in common research applications: ELISA, Immunocytochemistry/Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot. Each Nup53 Antibody is fully covered by our Guarantee+, to give you complete peace of mind and the support when you need it. Our Nup53 Antibodies can be used in a variety of model species: Bovine, Canine, Human, Mouse, Primate. Use the list below to choose the Nup53 Antibody which is most appropriate for your research; you can click on each one to view full technical details, images, references, reviews and related products. Choose from our Nup53 polyclonal antibodies.
Alternate Names for Nup53 Antibodies
anti-Nup53 antibody, anti-NUP35 antibody, anti-35 kDa nucleoporin antibody, anti-Mitotic phosphoprotein 44 antibody, anti-MP-44 antibody, anti-MP44NUP53 antibody, anti-NP44 antibody, anti-Nuclear pore complex protein Nup53 antibody, anti-nucleoporin 35kDa antibody, anti-Nucleoporin Nup35 antibody, anti-nucleoporin NUP53 antibody
6 Results for "nup53" in Primary Antibodies
![Western Blot: Nup53 Antibody [NB100-93322] - Whole cell lysate (50 ug) from HeLa, 293T, and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-NUP35 antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/Nup53-Antibody-Western-Blot-NB100-93322-img0010.jpg "<b>Biological Strategies Validation. </b>Western Blot: Nup53 Antibody [NB100-93322] - Whole cell lysate (50 ug) from HeLa, 293T, and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-NUP35 antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.")
![<b>Biological Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: Nup53 Antibody [NB100-93322] - Showing nuclear co-localization with p300 in PANC-1 cells using anti-Nup53 antibody (green). Image submitted by a verified customer review.](http://images.novusbio.com/fullsize/Nup53-Antibody-Immunocytochemistry-Immunofluorescence-NB100-93322-img0003.jpg)
![Immunohistochemistry-Paraffin: Nup53 Antibody [NB100-93322] - Sample: FFPE section of human colon carcinoma. Antibody: Affinity purified rabbit anti- NUP35 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/Nup53-Antibody-Immunohistochemistry-NB100-93322-img0011.jpg)
![<b>Independent Antibodies Validation. </b>Immunoprecipitation: Nup53 Antibody [NB100-93322] - Detection of human NUP35 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-NUP35 antibody NB100-93322 (lot NB100-93322-2) used for IP at 6 ug per reaction. NUP35 was also immunoprecipitated by a previous lot of this antibody (lot NB100-93322-1) and another rabbit anti-NUP35 antibody. For blotting immunoprecipitated NUP35, NB100-93322 was used at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/Nup53-Antibody-Immunoprecipitation-NB100-93322-img0012.jpg)
![Western Blot: Nup53 Antibody [NBP2-24637] - Analysis of NUP35 in human liver lysate in the 1) absence and 2) presence of immunizing peptide, 3) mouse brain lysate and 4) rat brain lysate using NBP2-24637. Goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.](http://images.novusbio.com/fullsize/Nup53-Antibody-Western-Blot-NBP2-24637-img0002.jpg "Western Blot: Nup53 Antibody [NBP2-24637] - Analysis of NUP35 in human liver lysate in the 1) absence and 2) presence of immunizing peptide, 3) mouse brain lysate and 4) rat brain lysate using NBP2-24637. Goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.")
![Immunohistochemistry-Paraffin: Nup53 Antibody [NBP2-24637] - Analysis of NUP35 in formalin-fixed, paraffin-embedded human brain tissue using an isotype control (top left) and this antibody (bottom left, right) at 5 ug/ml.](http://images.novusbio.com/fullsize/Nup53-Antibody-Immunohistochemistry-Paraffin-NBP2-24637-img0001.jpg)
![Western Blot: Nup53 Antibody [NBP1-92214] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp](http://images.novusbio.com/fullsize/Nup53-Antibody-Western-Blot-NBP1-92214-img0005.jpg "Western Blot: Nup53 Antibody [NBP1-92214] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp")
![Immunocytochemistry/Immunofluorescence: Nup53 Antibody [NBP1-92214] - Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm & nuclear membrane.](http://images.novusbio.com/fullsize/Nup53-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-92214-img0007.jpg)
![Immunohistochemistry-Paraffin: Nup53 Antibody [NBP1-92214] - Staining of human prostate shows strong positivity in nuclear membrane in glandular cells.](http://images.novusbio.com/fullsize/Nup53-Antibody-Immunohistochemistry-Paraffin-NBP1-92214-img0017.jpg)
![Immunohistochemistry-Paraffin: Nup53 Antibody [NBP1-92214] - Staining of human cerebellum shows strong positivity in nuclear membrane in Purkinje cells.](http://images.novusbio.com/fullsize/Nup53-Antibody-Immunohistochemistry-Paraffin-NBP1-92214-img0015.jpg)
![Immunohistochemistry-Paraffin: Nup53 Antibody [NBP1-92214] - Staining of human liver shows moderate positivity in nuclear membrane in hepatocytes.](http://images.novusbio.com/fullsize/Nup53-Antibody-Immunohistochemistry-Paraffin-NBP1-92214-img0016.jpg)
![Immunohistochemistry-Paraffin: Nup53 Antibody [NBP1-92214] - Staining of human testis shows strong positivity in nuclear membrane in cells in seminiferous ducts.](http://images.novusbio.com/fullsize/Nup53-Antibody-Immunohistochemistry-Paraffin-NBP1-92214-img0018.jpg)
![Western Blot: Nup53 Antibody [NBP1-92213] - Analysis in control (vector only transfected HEK293T lysate) and NUP35 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).](http://images.novusbio.com/fullsize/Nup53-Antibody-Western-Blot-NBP1-92213-img0007.jpg "Western Blot: Nup53 Antibody [NBP1-92213] - Analysis in control (vector only transfected HEK293T lysate) and NUP35 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).")
![Immunohistochemistry-Paraffin: Nup53 Antibody [NBP1-92213] - Staining of human hippocampus shows distinct positivity in nuclear membranes of neurons.](http://images.novusbio.com/fullsize/Nup53-Antibody-Immunohistochemistry-Paraffin-NBP1-92213-img0006.jpg)
![Western Blot: Nup53 Antibody [NBP1-92212] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp](http://images.novusbio.com/fullsize/Nup53-Antibody-Western-Blot-NBP1-92212-img0003.jpg "Western Blot: Nup53 Antibody [NBP1-92212] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp")
![Immunohistochemistry-Paraffin: Nup53 Antibody [NBP1-92212] - Staining of human adrenal gland shows strong nuclear and moderate cytoplasmic positivity in cortical cells.](http://images.novusbio.com/fullsize/Nup53-Antibody-Immunohistochemistry-Paraffin-NBP1-92212-img0001.jpg)
![Western Blot: Nup53 Antibody [NBP3-35269] - Western blot analysis of various lysates using Nup53 Rabbit pAb at 1:3000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 5s.](http://images.novusbio.com/fullsize/nbp3-35269_rabbit-nup53-pab-1012202411535833.jpg "Western Blot: Nup53 Antibody [NBP3-35269] - Western blot analysis of various lysates using Nup53 Rabbit pAb at 1:3000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 5s.")