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Numb Antibody (OTI1B5) [Janelia Fluor® 525]

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Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, IHC
Clone
OTI1B5
Clonality
Monoclonal
Host
Mouse
Conjugate
Janelia Fluor 525

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NBP2-73130AF350
Alexa Fluor 405
NBP2-73130AF405
Alexa Fluor 488
NBP2-73130AF488
Alexa Fluor 532
NBP2-73130AF532
Alexa Fluor 594
NBP2-73130AF594
Alexa Fluor 647
NBP2-73130AF647
Alexa Fluor 700
NBP2-73130AF700
Alexa Fluor 750
NBP2-73130AF750
Allophycocyanin
NBP2-73130APC
Biotin
NBP2-73130B
CoraFluor 1
NBP2-73130CL1
DyLight 350
NBP2-73130UV
DyLight 405
NBP2-73130V
DyLight 488
NBP2-73130G
DyLight 550
NBP2-73130R
DyLight 594
NBP2-73130DL594
DyLight 650
NBP2-73130C
DyLight 680
NBP2-73130FR
DyLight 755
NBP2-73130IR
FITC
NBP2-73130F
HRP
NBP2-73130H
Janelia Fluor 549
NBP2-73130JF549
Janelia Fluor 585
NBP2-73130JF585
Janelia Fluor 635
NBP2-73130JF635
Janelia Fluor 646
NBP2-73130JF646
Janelia Fluor 669
NBP2-73130JF669
PE
NBP2-73130PE
PerCP
NBP2-73130PCP
Unconjugated
NBP2-46284
View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
NBP2-46284
Azide and BSA Free
NBP2-73130
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Numb Antibody (OTI1B5) [Janelia Fluor® 525] Summary

Immunogen
Human recombinant protein fragment corresponding to amino acids 1-365 of human NUMB (NP_001005744) produced in E.coli.
Isotype
IgG2a
Clonality
Monoclonal
Host
Mouse
Gene
NUMB
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Western Blot
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Immunogen affinity purified

Notes



Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus.

Alternate Names for Numb Antibody (OTI1B5) [Janelia Fluor® 525]

  • h-Numb
  • mnb
  • m-numb
  • numb (Drosophila) homolog
  • numb homolog (Drosophila)
  • Numb
  • protein numb homolog
  • Protein S171
  • S171

Background

Numb is a transmembrane signaling receptor that is activated by the DSL family of ligands. Studies suggestthat mammalian Numb plays a role in progenitor cell fate in neurogenesis. Numb exerts its role on cell fate by antagonizing Notch signaling. The mammalian Numb gene undergoes alternative splicing to produce at least four functionally different Numb isoforms which regulate different processes. A loss of Numb expression has been demonstrated in breast cancer, non-small cell lung cancer and chronic myelogenous leukemia.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Numb Antibody
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NBP2-73130JF525
Numb Antibody (OTI1B5) [Janelia Fluor® 525]
Species: Hu, Mu, Rt
Applications: WB, IHC

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Product General Protocols

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol NUMB