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NUCKS1 Antibody (3G10)

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Immunocytochemistry/ Immunofluorescence: NUCKS1 Antibody (3G10) [H00064710-M02] - Analysis of monoclonal antibody to NUCKS1 on HeLa cell . Antibody concentration 10 ug/ml.
Sandwich ELISA: NUCKS1 Antibody (3G10) [H00064710-M02] - Detection limit for recombinant GST tagged NUCKS1 is 0.3 ng/ml as a capture antibody.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, ICC/IF
Clone
3G10
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

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NUCKS1 Antibody (3G10) Summary

Immunogen
NUCKS1 (NP_073568.2, 1 a.a. ~ 243 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MSRPVRNRKVVDYSQFQESDDADEDYGRDSGPPTKKIRSSPREAKNKRRSGKNSQEDSEDSEDKDVKTKKDDSHSAEDSEDEKEDHKNVRQQRQAASKAASKQREMLMEDVGSEEEQEEEDEAPFQEKDSGSDEDFLMEDDDDSDYGSSKKKNKKMVKKSKPERKEKKMPKPRLKATVTPSPVKGKGKVGRPTASKASKEKTPSPKEEDEEPESPPEKKTSTSPPPEKSGDEGSEDEAPSGED
Specificity
This product is specific for Human NUCKS1 monoclonal antibody (M02), clone 3G10 [Gene ID: 64710].
Isotype
IgG2a Kappa
Clonality
Monoclonal
Host
Mouse
Gene
NUCKS1
Purity
IgG purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • ELISA
  • Immunocytochemistry/ Immunofluorescence 10 ug/ml
  • Sandwich ELISA
  • Western Blot
Application Notes
Mouse monoclonal antibody raised against a full-length recombinant NUCKS1.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
In 1x PBS, pH 7.4
Preservative
No Preservative
Purity
IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for NUCKS1 Antibody (3G10)

  • FLJ21480
  • FLJ32016
  • FLJ38536
  • NUCKSnuclear ubiquitous casein and cyclin-dependent kinases substrate
  • nuclear casein kinase and cyclin-dependent kinase substrate 1
  • nuclear ubiquitous casein kinase and cyclin-dependent kinase substrate
  • P1
  • potential LAG1 interactor

Background

NUCKS (nuclear ubiquitous casein and cyclin-dependent kinases substrate) is a nuclear phosphoprotein phosphorylated by Cdk1 during mitosis. It is a DNA-binding protein and bears two putative nuclear localization signals. NUCKS has been shown to localize to the cytoplasm in mitotic cells and is targeted to the nucleus during telophase. It is expressed ubiquitously and possesses features of a housekeeping gene. Alternate names for NUCKS include NUCKS1, JC7, and FLJ21480.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for NUCKS1 Antibody (H00064710-M02). (Showing 1 - 1 of 1 FAQ).

  1. If we receive a small trial aliquot of NUCKS1 antibody (3G10), cat. no H00064710-M02, we can support you with documentation (pictures) if the antibody works well. We have long experience with working on the NUCKS1 protein, including immune cytochemistry, Western immuno-blotting and immune precipitation.
    • Our NUCKS1 antibody, catalog number H00064710-M02, has been validated for Western blotting and ELISA with human samples. Unfortunately we are unable to send out free samples of this product; however you may be interested in our Innovator's Reward Program. This program was designed to help scientists with the cost of their antibodies in return for valuable information. If you decide to test an antibody in a previously untested species or application we will issue you a 100% credit for the purchase price of the antibody in exchange for a review of your experiment using our product.
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Bioinformatics

Gene Symbol NUCKS1
Uniprot