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NFYC Antibody (1D3)

Images

 
Western Blot: NFYC Antibody (1D3) [H00004802-M01] - Analysis of NFYC expression in transfected 293T cell line by NFYC monoclonal antibody (M01), clone 1D3.Lane 1: NFYC transfected lysate(37.2 KDa).Lane 2: ...read more
Immunocytochemistry/ Immunofluorescence: NFYC Antibody (1D3) [H00004802-M01] - Analysis of monoclonal antibody to NFYC on HeLa cell . Antibody concentration 10 ug/ml.
Immunohistochemistry-Paraffin: NFYC Antibody (1D3) [H00004802-M01] - Analysis of monoclonal antibody to NFYC on formalin-fixed paraffin-embedded human testis. Antibody concentration 3 ug/ml.
Western Blot: NFYC Antibody (1D3) [H00004802-M01] - Analysis of NFYC over-expressed 293 cell line, cotransfected with NFYC Validated Chimera RNAi ( Cat # H00004802-R01V ) (Lane 2) or non-transfected control (Lane 1). ...read more
ELISA: NFYC Antibody (1D3) [H00004802-M01] - Detection limit for recombinant GST tagged NFYC is 3 ng/ml as a capture antibody.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, ICC/IF, IHC, KD
Clone
1D3
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

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NFYC Antibody (1D3) Summary

Description
Quality control test: Antibody Reactive Against Recombinant Protein.
Immunogen
NFYC (AAH05003, 14 a.a. ~ 113 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. DAQQSLQSFWPRVMEEIRNLTVKDFRVQELPLARIKKIMKLDEDVKMISAEAPVLFAKAAQIFITELTLRAWIHTEDNKRRTLQRNDIAMAITKFDQFDF
Specificity
NFYC - nuclear transcription factor Y, gamma
Isotype
IgG3 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
NFYC
Purity
IgG purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • ELISA
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Knockdown Validated
  • Western Blot 1:500
Application Notes
Antibody reactivity against recombinant protein for WB. It has been used for IHC-P, RNAi Validation and ELISA.
Publications
Read Publication using
H00004802-M01 in the following applications:

  • WB
    1 publication

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
In 1x PBS, pH 7.4
Preservative
No Preservative
Purity
IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for NFYC Antibody (1D3)

  • C subunit
  • nuclear transcription factor Y, gamma

Background

This gene encodes one subunit of a trimeric complex forming a highly conserved transcription factor that binds with high specificity to CCAAT motifs in the promoters of a variety of genes. The encoded protein, subunit C, forms a tight dimer with the B subunit, a prerequisite for subunit A association. The resulting trimer binds to DNA with high specificity and affinity. Subunits B and C each contain a histone-like motif. Observation of the histone nature of these subunits is supported by two types of evidence; protein sequence alignments and experiments with mutants. Additional regulation, preliminarily supported by the EST database, may be represented by alternative splicing in this subunit.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for NFYC Antibody (H00004802-M01)(1)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: WB.


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Video Protocols

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ICC/IF Video Protocol

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Bioinformatics

Gene Symbol NFYC
Entrez
OMIM
Uniprot