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NEK1 Recombinant Protein Antigen

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Reactivity HuSpecies Glossary
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NEK1 Recombinant Protein Antigen Summary

Description
A recombinant protein antigen with a N-terminal His6-ABP tag corresponding to human NEK1.

Source: E. coli

Amino Acid Sequence: VLKEQLERKRKEAYEREKKVWEEHLVAKGVKSSDVSPPLGQHETGGSPSKQQMRSVISVTSALKEVGVDSSLTDTRETSEEMQKTNNAISSKR

Fusion Tag: N-terminal His6ABP (ABP = Albumin Binding Protein derived from Streptococcal Protein G)

This product is intended to be used as a blocking antigen for antibody competition assays. Any other use of this antigen is done at the risk of the user. The use of this product for commercial production is strictly prohibited. Please contact technical support if you have any questions.
Source
E. coli
Protein/Peptide Type
Recombinant Protein Antigen
Gene
NEK1
Purity
>80% by SDS-PAGE and Coomassie blue staining

Applications/Dilutions

Dilutions
  • Antibody Competition 10 - 100 molar excess
Application Notes
This recombinant antigen is only intended to be used as a blocking agent to confirm antibody specificity with the corresponding antibody, catalog number NBP1-82883.

It is purified by IMAC chromatography, and the expected concentration is greater than 0.5 mg/ml.

For current lot information, including availability, please contact our technical support team click nb-technical@bio-techne.com

Theoretical MW
28 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS and 1M Urea, pH 7.4.
Preservative
No Preservative
Purity
>80% by SDS-PAGE and Coomassie blue staining

Alternate Names for NEK1 Recombinant Protein Antigen

  • DKFZp686D06121
  • DKFZp686K12169
  • EC 2.7.11
  • EC 2.7.11.1
  • KIAA1901MGC138800
  • Never in mitosis A-related kinase 1
  • NIMA (never in mitosis gene a)-related kinase 1
  • NimA-related protein kinase 1
  • NY-REN-55
  • protein-serine/threonine kinase
  • Renal carcinoma antigen NY-REN-55
  • serine/threonine-protein kinase Nek1
  • SRPS2

Background

Belonging to the NEK Ser/Thr protein kinase family, NEK1 plays an important role in the cell by responding to DNA damage. Molecular functions for NEK1 include protein serine/threonine kinase activity, protein tyrosine kinase activity, protein binding and ATP binding. More specifically, NEK1 is involved in cell cycle regulation, and the protein encoded by this gene is a serine/threonine kinase which is found in a centrosomal complex with FEZ1. Additionally, NEK1 also appears to possess tyrosine kinase activity and is involved in the control of meiosis and cilium assembly. NEK1 is known to interact with FEZ1, FEZ2, KIF3A, TSC2 and MRE11A. Common diseases for NEK1 include adenocarcinoma, cancer, short rib-polydactyly syndrome II and epithelial neoplasia.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

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Bioinformatics

Gene Symbol NEK1