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NEDD1 Antibody (7D10)

Images

 
Western Blot: NEDD1 Antibody (7D10) [H00121441-M05] - Western Blot analysis of NEDD1 expression in NIH/3T3 ( Cat # L018V1 ).
Immunohistochemistry-Paraffin: NEDD1 Antibody (7D10) [H00121441-M05] - Analysis of monoclonal antibody to NEDD1 on formalin-fixed paraffin-embedded human placenta. Antibody concentration 0.75 ug/ml
Western Blot: NEDD1 Antibody (7D10) [H00121441-M05] - Western Blot analysis of NEDD1 expression in Hela NE ( Cat # L013V3 ).
ELISA: NEDD1 Antibody (7D10) [H00121441-M05] - Detection limit for recombinant GST tagged NEDD1 is approximately 0.03ng/ml as a capture antibody.

Product Details

Summary
Reactivity Hu, Mu, Rt, Po, Bv, ShSpecies Glossary
Applications WB, ELISA, ICC/IF, IHC, WB
Clone
7D10
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

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NEDD1 Antibody (7D10) Summary

Immunogen
NEDD1 (NP_690869, 561 a.a. ~ 660 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. AGVASSLSEKIADSIGNNRQNAPLTSIQIRFIQNMIQETLDDFREACHRDIVNLQVEMIKQFHMQLNEMHSLLERYSVNEGLVAEIERLREENKRLRAHF
Marker
Centrosome Marker
Specificity
NEDD1 - neural precursor cell expressed, developmentally down-regulated 1
Isotype
IgG2b Kappa
Clonality
Monoclonal
Host
Mouse
Gene
NEDD1
Purity
IgG purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • ELISA 1:100-1:2000
  • Immunoblotting
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Western Blot 1:500
Application Notes
Antibody reactive against cell lysate and recombinant protein for Western Blot. Has also been used for immunohistochemistry (paraffin) and ELISA. Use in Immunocytochemistry/immunofluorescence reported in scientific literature (PMID: 24025740).
Publications
Read Publications using
H00121441-M05 in the following applications:

Reactivity Notes

Other species have not been tested.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
In 1x PBS, pH 7.4
Preservative
No Preservative
Purity
IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for NEDD1 Antibody (7D10)

  • FLJ35902
  • GCP-WD
  • NEDD-1
  • Neural precursor cell expressed developmentally down-regulated protein 1
  • neural precursor cell expressed, developmentally down-regulated 1
  • protein NEDD1
  • TUBGCP7

Background

NEDD1 is required for mitosis progression. Promotes the nucleation of microtubules from the spindle

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Applications: WB, ELISA, ICC/IF, IHC, WB

Publications for NEDD1 Antibody (H00121441-M05)(13)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 2 applications: ICC/IF, WB.


Filter By Application
ICC/IF
(1)
WB
(1)
All Applications
Filter By Species
Mouse
(2)
All Species
Showing Publications 1 - 10 of 13. Show All 13 Publications.
Publications using H00121441-M05 Applications Species
Krystal P, Jacopo S, Isabelle V et al. NEDD1-S411 phosphorylation plays a critical function in the coordination of microtubule nucleation during mitosis. Biol Open. 2022-10-31 [PMID: 36318115]
Isabell S, Marta R, M H et al. Dual spindles assemble in bovine zygotes despite the presence of paternal centrosomes. J Cell Biol. 2021-09-22 [PMID: 34550316]
Baumann C, Wang X, Yang L, Viveiros MM. Error-prone meiotic division and subfertility in mice with oocyte-conditional knockdown of pericentrin. J. Cell. Sci. 2017-02-13 [PMID: 28193732] (Mouse) Mouse
Uehara R, Kamasaki T, Hiruma S et al. Augmin shapes the anaphase spindle for efficient cytokinetic furrow ingression and abscission. Mol Biol Cell 2016-03-01 [PMID: 26764096] (WB) WB
Lecland N, Luders J. The dynamics of microtubule minus ends in the human mitotic spindle. Nat Cell Biol. 2014-06-29 [PMID: 24976384]
Tamura AN, Huang TT, Marikawa Y. Impact of Vitrification on the Meiotic Spindle and Components of the Microtubule-Organizing Center in Mouse Mature Oocytes. Biol Reprod. 2013-09-11 [PMID: 24025740] (ICC/IF, Mouse) ICC/IF Mouse
Uehara R, Goshima G. Functional central spindle assembly requires de novo microtubule generation in the interchromosomal region during anaphase. Uehara R, Goshima G. [PMID: 20937700]
Teixido-Travesa N, Villen J, Lacasa C et al. The {gamma}TuRC Revisited: A Comparative Analysis of Interphase and Mitotic Human {gamma}TuRC Redefines the Set of Core Components and Identifies the Novel Subunit GCP8. Mol Biol Cell 2010-09-24 [PMID: 20861304]
Archinti M, Lacasa C, Teixido-Travesa N, Luders J. SPICE--a previously uncharacterized protein required for centriole duplication and mitotic chromosome congression. J Cell Sci 123(Pt 18):3039-46. 2010-09-15 [PMID: 20736305]
Stiess M, Maghelli N, Kapitein LC et al. Axon extension occurs independently of centrosomal microtubule nucleation. Science 327(5966):704-7. 2010-02-05 [PMID: 20056854]
Show All 13 Publications.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

Additional NEDD1 Products

Array H00121441-M05

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Bioinformatics

Gene Symbol NEDD1
Entrez
OMIM
Uniprot