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Mouse Pro-MMP-9 Quantikine ELISA Kit

 (15 Publications)
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Summary
Reactivity MuSpecies Glossary
Applications ELISA
Conjugate
HRP

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Mouse Pro-MMP-9 Quantikine ELISA Kit Summary

Background
The Quantikine Mouse Pro-MMP-9 Immunoassay is a 4.5 hour solid phase ELISA designed to measure free and TIMP-1 bound mouse Pro-MMP-9 in cell culture supernates, serum, and platelet-poor plasma. It contains NS0-expressed recombinant mouse Pro-MMP-9 and antibodies raised against the recombinant factor. Natural mouse Pro-MMP-9 showed dose-response curves that were parallel to the standard curve...s obtained using the recombinant Quantikine kit standards, indicating that this kit can be used to determine relative levels of natural mouse Pro-MMP-9.
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Specificity
Natural and recombinant mouse Pro-MMP-9. This kit recognizes Pro-MMP-9 when complexed with TIMP-1 but does not recognize active mouse MMP-9.
Source
N/A
Inter-Assay
See PDF Datasheet for details
Intra-Assay
See PDF Datasheet for details
Spike Recovery
See PDF Datasheet for details
Sample Volume
See PDF Datasheet for details
Gene
Mmp9

Applications/Dilutions

Dilutions
  • ELISA
Application Notes
No significant interference observed with available related molecules.
Publications
Read Publications using MMP900B.

Packaging, Storage & Formulations

Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Mouse Pro-MMP-9 Quantikine ELISA Kit

  • 92 kDa gelatinase
  • 92 kDa type IV collagenase
  • CLG4B
  • EC 3.4.24
  • EC 3.4.24.35
  • Gelatinase B
  • GELB
  • macrophage gelatinase
  • MANDP2
  • matrix metallopeptidase 9
  • matrix metalloproteinase 9
  • matrix metalloproteinase-9
  • MMP9
  • MMP-9
  • type V collagenase

Background

The matrix metalloproteinases (MMPs) consist of 24 known human zinc proteases with essential roles in breaking down components of the extracellular matrix (ECM). Additional MMP substrates include cytokines, chemokines, growth factors and binding proteins, cell/cell adhesion molecules, and other proteinases. With a few exceptions, MMPs share common structural motifs including a pro-peptide domain, a catalytic domain, a hinge region, and a hemopexin-like domain. Synthesized as pro-enzymes, most MMPs are secreted before conversion to their active form. MMP activities are modulated on several levels including transcription, pro-enzyme activation, or by their endogenous inhibitors, tissue inhibitors of metalloproteinases (TIMPs). A subset of MMPs are associated with membranes and designated as membrane-type metalloproteinases (MT-MMP).

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⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Publications for MMP-9 (MMP900B)(15)

We have publications tested in 1 confirmed species: Mouse.


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Mouse
(15)
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Showing Publications 1 - 10 of 15. Show All 15 Publications.
Publications using MMP900B Applications Species
A Spiegel, MW Brooks, S Houshyar, F Reinhardt, M Ardolino, E Fessler, MB Chen, JA Krall, J DeCock, IK Zervantona, A Iannello, Y Iwamoto, V Cortez-Ret, RD Kamm, MJ Pittet, DH Raulet, RA Weinberg Neutrophils suppress intraluminal NK-mediated tumor cell clearance and enhance extravasation of disseminated carcinoma cells Cancer Discov, 2016-04-12;0(0):. 2016-04-12 [PMID: 27072748] (Mouse) Mouse
Toki S, Goleniewska K, Huckabee M, Zhou W, Newcomb D, FitzGerald G, Lawson W, Peebles R PGI(2) signaling inhibits antigen uptake and increases migration of immature dendritic cells. J Leukoc Biol, 2013-04-26;94(1):77-88. 2013-04-26 [PMID: 23625201] (Mouse) Mouse
Awla D, Abdulla A, Syk I, Jeppsson B, Regner S, Thorlacius H Neutrophil-derived matrix metalloproteinase-9 is a potent activator of trypsinogen in acinar cells in acute pancreatitis. J. Leukoc. Biol., 2011-11-18;91(5):711-9. 2011-11-18 [PMID: 22100390] (Mouse) Mouse
Jin W, Chen BB, Li JY, Zhu H, Huang M, Gu SM, Wang QQ, Chen JY, Yu S, Wu J, Shao ZM TIEG1 inhibits breast cancer invasion and metastasis by inhibition of epidermal growth factor receptor (EGFR) transcription and the EGFR signaling pathway. Mol. Cell. Biol., 2011-10-24;32(1):50-63. 2011-10-24 [PMID: 22025675] (Mouse) Mouse
Nannuru KC, Futakuchi M, Varney ML Matrix metalloproteinase (MMP)-13 regulates mammary tumor-induced osteolysis by activating MMP9 and transforming growth factor-beta signaling at the tumor-bone interface. Cancer Res., 2010-04-20;70(9):3494-504. 2010-04-20 [PMID: 20406980] (Mouse) Mouse
Yen JH, Kong W, Ganea D IFN-beta inhibits dendritic cell migration through STAT-1-mediated transcriptional suppression of CCR7 and matrix metalloproteinase 9. J. Immunol., 2010-02-26;184(7):3478-86. 2010-02-26 [PMID: 20190134] (Mouse) Mouse
Kim DY, Park JW, Jeoung D, Ro JY Celastrol suppresses allergen-induced airway inflammation in a mouse allergic asthma model. Eur. J. Pharmacol., 2009-04-05;612(1):98-105. 2009-04-05 [PMID: 19356734] (Mouse) Mouse
Simoes DC, Xanthou G, Petrochilou K, Panoutsakopoulou V, Roussos C, Gratziou C Osteopontin deficiency protects against airway remodeling and hyperresponsiveness in chronic asthma. Am. J. Respir. Crit. Care Med., 2009-02-20;179(10):894-902. 2009-02-20 [PMID: 19234104] (Mouse) Mouse
Lackey DE, Ashley SL, Davis AL, Hoag KA Retinoic acid decreases adherence of murine myeloid dendritic cells and increases production of matrix metalloproteinase-9. J. Nutr., 2008-08-01;138(8):1512-9. 2008-08-01 [PMID: 18641199] (Mouse) Mouse
Damsker JM, Okwumabua I, Pushkarsky T, Arora K, Bukrinsky MI, Constant SL Targeting the chemotactic function of CD147 reduces collagen-induced arthritis. Immunology, 2008-06-14;126(1):55-62. 2008-06-14 [PMID: 18557953] (Mouse) Mouse
Show All 15 Publications.

Reviews for MMP-9 (MMP900B) (0)

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for MMP-9 (MMP900B). (Showing 1 - 1 of 1 FAQs).

  1.  I’m looking for a pair of antibodies to MMP-9 that can be used in a sandwich assay. Do you carry any? 
    • We have 10 primary antibodies for MMP-9 that have been tested in ELISA, seen here.It looks like 2 have been tested for capture (please note the tested species for each of these), seen here.6 have been tested for detection, seen here.

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Bioinformatics

Gene Symbol Mmp9