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MMP-9 Antibody (36020) [Unconjugated]

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MMP-9 was detected in immersion fixed SW1353 human chondrosarcoma cell line using Mouse Anti-Human/Primate MMP-9 Monoclonal Antibody (Catalog # MAB936) at 3 µg/mL for 3 hours at room temperature. Cells were stained ...read more
MMP‑9 was detected in immersion fixed paraffin-embedded sections of human lung using Mouse Anti-Human/Primate MMP‑9 Monoclonal Antibody (Catalog # MAB936) at 15 µg/mL for 1 hour at room temperature followed ...read more

Product Details

Summary
Reactivity Hu, PmSpecies Glossary
Applications WB, IHC, IP, ELISA(Cap), ICC/IF
Clone
36020
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

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MMP-9 Antibody (36020) [Unconjugated] Summary

Immunogen
Chinese hamster ovary cell line CHO-derived recombinant human MMP-9
Specificity
Detects human MMP-9 in Western blots. Does not react with the C-terminal truncated form (65 kDa) of recombinant human (rh) MMP-9. In Western blots, approximately 5% cross-reactivity with rhMMP-2 and no cross-reactivity with rhMMP-1 or rhMMP-3 is observed.
Source
N/A
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
MMP9
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • ELISA Capture (Matched Antibody Pair) 2-8 ug/mL
  • ELISA Detection (Matched Antibody Pair) 0.1-0.4 ug/mL
  • ELISA Standard (Matched Pair)
  • Immunocytochemistry 3-25 ug/mL
  • Immunohistochemistry 8-25 ug/mL
  • Immunoprecipitation 25 ug/mL
  • Western Blot 1 ug/mL
Application Notes
ELISA Detection: Human MMP-9 Biotinylated Antibody (Catalog number BAF911)
Standard: Recombinant Human MMP-9 (Catalog number 911-MP)
Publications
Read Publications using
MAB936 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for MMP-9 Antibody (36020) [Unconjugated]

  • 92 kDa gelatinase
  • 92 kDa type IV collagenase
  • CLG4B
  • EC 3.4.24
  • EC 3.4.24.35
  • Gelatinase B
  • GELB
  • macrophage gelatinase
  • MANDP2
  • matrix metallopeptidase 9
  • matrix metalloproteinase 9
  • matrix metalloproteinase-9
  • MMP9
  • MMP-9
  • type V collagenase

Background

Pro Matrix Metalloproteinase 9 (Pro-MMP-9) is an inactive proenzyme secreted by multiple cell types. The N-terminal pro region is proteolytically removed, resulting in active MMP-9, which can degrade collagens and elastin as well as several non-ECM molecules.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for MMP-9 Antibody (MAB936)(15)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 6 applications: ELISA Capture, ELISA Development (Capture), Flow Cytometry, IHC, IHC-P, Western Blot.


Filter By Application
ELISA Capture
(1)
ELISA Development (Capture)
(1)
Flow Cytometry
(1)
IHC
(1)
IHC-P
(1)
Western Blot
(5)
All Applications
Filter By Species
Human
(10)
All Species
Showing Publications 1 - 10 of 15. Show All 15 Publications.
Publications using MAB936 Applications Species
Barbu, E;Mihaila, AC;Gan, AM;Ciortan, L;Macarie, RD;Tucureanu, MM;Filippi, A;Stoenescu, AI;Petrea, SV;Simionescu, M;Balanescu, SM;Butoi, E; The Elevated Inflammatory Status of Neutrophils Is Related to In-Hospital Complications in Patients with Acute Coronary Syndrome and Has Important Prognosis Value for Diabetic Patients International journal of molecular sciences 2024-05-08 [PMID: 38791147] (Western Blot, Human) Western Blot Human
J Kudelski, A Tokarzewic, M Gudowska-S, B Mroczko, P Ch?osta, M Bruczko-Go, P Mitura, G M?ynarczyk The Significance of Matrix Metalloproteinase 9 (MMP-9) and Metalloproteinase 2 (MMP-2) in Urinary Bladder Cancer Biomedicines, 2023-03-20;11(3):. 2023-03-20 [PMID: 36979935] (Western Blot, Human) Western Blot Human
Maile Velasquez, Chris O’Sullivan, Robert Brockett, Amanda Mikels-Vigdal, Igor Mikaelian, Victoria Smith et al. Characterization of Active MMP9 in Chronic Inflammatory Diseases Using a Novel Anti-MMP9 Antibody Antibodies (Basel) 2023-01-18 [PMID: 36810514]
Difang Sun, Zongyi Zhan, Rui Zeng, Xiaolin Liu, Bin Wang, Fan Yang et al. Long-term and potent IOP-lowering effect of I kappa B alpha -siRNA in a nonhuman primate model of chronic ocular hypertension iScience 2022-04-15 [PMID: 35445186]
Yoshihiro Inamoto, Paul J. Martin, Lynn E. Onstad, Guang-Shing Cheng, Kirsten M. Williams, Iskra Pusic et al. Relevance of Plasma Matrix Metalloproteinase-9 for Bronchiolitis Obliterans Syndrome after Allogeneic Hematopoietic Cell Transplantation Transplantation and Cellular Therapy 2021-09-01 [PMID: 34126278]
W Na, EJ Lee, MK Kang, YH Kim, DY Kim, H Oh, SI Kim, SY Oh, YH Kang Aesculetin Inhibits Osteoclastic Bone Resorption through Blocking Ruffled Border Formation and Lysosomal Trafficking Int J Mol Sci, 2020-11-13;21(22):. 2020-11-13 [PMID: 33203061] (Western Blot, Human) Western Blot Human
L Tiainen, M Hämäläinen, T Luukkaala, M Tanner, O Lahdenperä, P Vihinen, A Jukkola, P Karihtala, E Moilanen, PL Kellokumpu Low Plasma IL-8 Levels During Chemotherapy Are Predictive of Excellent Long-Term Survival in Metastatic Breast Cancer Clin. Breast Cancer, 2019-04-04;0(0):. 2019-04-04 [PMID: 31029558] (ELISA Capture, Human) ELISA Capture Human
SIN-HYE PARK, MIN JAE SHIN, DAE WON KIM, JINSEU PARK, SOO YOUNG CHOI, YOUNG-HEE KANG Blockade of monocyte-endothelial trafficking by transduced Tat-superoxide dismutase protein International Journal of Molecular Medicine 2016-02-01 [PMID: 26707483]
Ohlsson S, Ohlsson S, Soderberg D, Gunnarsson L, Pettersson A, Segelmark M, Hellmark T Neutrophils from vasculitis patients exhibit an increased propensity for activation by anti-neutrophil cytoplasmic antibodies. Clin Exp Immunol, 2014-06-01;176(3):363-72. 2014-06-01 [PMID: 24666336] (ELISA Development (Capture), Human) ELISA Development (Capture) Human
Elena Kostova, Maja Slaninka-Miceska, Nikola Labacevski, Krume Jakovski, Jasmina Trojachanec, Emilija Atanasovska et al. Expression of matrix metalloproteinases 2, 7 and 9 in patients with colorectal cancer Vojnosanitetski pregled 2014-02-13 [PMID: 24516991]
Show All 15 Publications.

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Product General Protocols

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FAQs for MMP-9 Antibody (MAB936). (Showing 1 - 1 of 1 FAQs).

  1.  I’m looking for a pair of antibodies to MMP-9 that can be used in a sandwich assay. Do you carry any? 
    • We have 10 primary antibodies for MMP-9 that have been tested in ELISA, seen here.It looks like 2 have been tested for capture (please note the tested species for each of these), seen here.6 have been tested for detection, seen here.

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol MMP9