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MMP-2 Antibody (8B4) - (Pro and Active) - Azide and BSA Free

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Western Blot: MMP-2 Antibody (8B4) - (Pro and Active) - Azide and BSA Free [NBP2-80854] - Analysis of MMP2 expression in ProMMP2 (1), Active MMP2 (2) and human pressure ulcer biopsy (3). Image from the standard format of this antibody.
Western Blot: MMP-2 Antibody (8B4) - (Pro and Active) - Azide and BSA Free [NBP2-80854] - Analysis of MMP2 expression in ProMMP2 (1), Active MMP2 (2) and human pressure ulcer biopsy (3). Image from the standard format ...read more
Immunohistochemistry: MMP-2 Antibody (8B4) - (Pro and Active) - Azide and BSA Free [NBP2-80854] - Detection of MMP-2 staining in FFPE human ovary tissue. Primary antibody was used at 2.5 ug/ml with the ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ELISA, ICC/IF, IHC, IP, KD
Clone
8B4
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
Azide and BSA Free
Concentration
1 mg/ml

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NB200-114AF350
Alexa Fluor 405
NB200-114AF405
Alexa Fluor 488
NB200-114AF488
Alexa Fluor 532
NB200-114AF532
Alexa Fluor 594
NB200-114AF594
Alexa Fluor 647
NB200-114AF647
Alexa Fluor 700
NB200-114AF700
Alexa Fluor 750
NB200-114AF750
Biotin
NB200-114B
CoraFluor 1
NB200-114CL1
DyLight 350
NB200-114UV
DyLight 405
NB200-114V
DyLight 488
NB200-114G
DyLight 550
NB200-114R
DyLight 594
NB200-114DL594
DyLight 650
NB200-114C
DyLight 680
NB200-114FR
DyLight 755
NB200-114IR
FITC
NB200-114F
HRP
NB200-114H
Janelia Fluor 525
NB200-114JF525
Janelia Fluor 549
NB200-114JF549
Janelia Fluor 585
NB200-114JF585
Janelia Fluor 635
NB200-114JF635
Janelia Fluor 646
NB200-114JF646
Janelia Fluor 669
NB200-114JF669
Unconjugated
NB200-114
mFluor Violet 450 SE
NB200-114MFV450
mFluor Violet 500 SE
NB200-114MFV500
mFluor Violet 610 SE
NB200-114MFV610
View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
NB200-114
BSA Free
NB200-114
BSA Free
NB200-114SS
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MMP-2 Antibody (8B4) - (Pro and Active) - Azide and BSA Free Summary

Immunogen
Activated recombinant human MMP-2. [Uniprot: P08253]
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
MMP2
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
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Applications/Dilutions

Dilutions
  • ELISA 0.03 ug/ml
  • Immunocytochemistry/ Immunofluorescence 1:10-1:500
  • Immunohistochemistry 2 ug/ml
  • Immunohistochemistry-Frozen 2ug/ml
  • Immunohistochemistry-Paraffin 2 ug/ml
  • Immunoprecipitation
  • Knockdown Validated
  • Western Blot 1-2ug/ml
Application Notes
In Western blot, a band is seen at 70 and 62kDa representing the MMP2 band-pair. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
No Preservative
Concentration
1 mg/ml
Purity
Protein G purified

Alternate Names for MMP-2 Antibody (8B4) - (Pro and Active) - Azide and BSA Free

  • 72 kDa gelatinase
  • CLG4
  • CLG4A72 kDa type IV collagenase
  • collagenase type IV-A
  • EC 3.4.24
  • EC 3.4.24.24
  • Gelatinase A
  • matrix metallopeptidase 2 (gelatinase A, 72kDa gelatinase, 72kDa type IVcollagenase)
  • matrix metalloproteinase 2 (gelatinase A, 72kD gelatinase, 72kD type IVcollagenase)
  • Matrix metalloproteinase-2
  • matrix metalloproteinase-II
  • MMP2
  • MMP-2
  • MMP-II
  • MONA
  • neutrophil gelatinase
  • TBE-1matrix metalloproteinase 2 (gelatinase A, 72kDa gelatinase, 72kDa type IVcollagenase)

Background

MMP2 is a member of the Matrix Metalloproteinase (MMP) family, which are involved in extracellular matrix degradation under both normal physiological and disease processes. Specifically, MMP-2 is responsible for degrading collagens type IV, V, VII, and X and gelatin type I. Other reported functions of MMP 2 include angiogenesis, tumor invasion, tissue repair, remodeling of the vasculature, inflammation, and atherosclerotic plaque rupture. MMP-2 secretion is activated by the Ras signaling pathway. The MMP2 antibody clone MMP2/8B4 was created against activated recombinant human MMP2, and will specifcally detect both the active and inactive forms. This MMP-2 antibody has been thoroughly characterized in most common immunological applications and has been cited in a number of published journal articles.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol MMP2