Home » MMP-14/MT1-MMP » MMP-14/MT1-MMP Antibodies » MMP-14/MT1-MMP Antibody (5H2) [Unconjugated]
Novus Biologicals products are now on bio-techne.com

MMP-14/MT1-MMP Antibody (5H2) [Unconjugated]

 (3 Publications)
 (1 Review)
 See All Related
Submit a Review

Images

 
(
Enlarge)
Western blot shows lysates of human placenta tissue. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human MMP-14/MT1-MMP Monoclonal Antibody (Catalog # MAB918) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). Specific bands were detected for MMP-14/MT1-MMP at approximately 50 kDa and 62 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Western blot shows lysates of human placenta tissue. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human MMP-14/MT1-MMP Monoclonal Antibody (Catalog # MAB918) followed by HRP-conjugated Anti-Mouse IgG Secondary ...read more
MMP-14 was detected in immersion fixed paraffin-embedded sections of human benign nodular hyperplasia using 25 µg/mL Mouse Anti-Human MMP-14 Monoclonal Antibody (Catalog # MAB918) overnight at 4 °C. Tissue was stained ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC, IP
Clone
5H2
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
MAB918-SP
Novus Biologicals is part of Bio-Techne

Shop this product on bio-techne.com

Novus Biologicals, part of Bio-Techne, offers 100% Guarantee

MMP-14/MT1-MMP Antibody (5H2) [Unconjugated] Summary

Immunogen
Human MMP-14 synthetic peptide
AYIREGHEKQA
Accession # P50281
Specificity
Detects recombinant human (rh) MMP-14 in Western blots. In Western blots, no cross-reactivity with recombinant human (rh) MMP-1, -2, -7,
-8, -9, -10, -12, -13 or the catalytic domains of rhMMP-15 and -16.
Source
N/A
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
MMP14
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Immunohistochemistry 8-25 ug/mL
  • Immunoprecipitation 25 ug/mL
  • Western Blot 2 ug/mL
Reviewed Applications
Read 1 Review rated 4
using
MAB918 in the following applications:
Publications
Read Publications using
MAB918 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in TBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for MMP-14/MT1-MMP Antibody (5H2) [Unconjugated]

  • EC 3.4.24
  • EC 3.4.24.80
  • matrix metallopeptidase 14 (membrane-inserted)
  • matrix metalloproteinase 14 (membrane-inserted)
  • matrix metalloproteinase-14
  • membrane type 1 metalloprotease
  • Membrane-type matrix metalloproteinase 1
  • Membrane-type-1 matrix metalloproteinase
  • MMP14
  • MMP-14
  • MMP-X1
  • MT1MMP
  • MT1-MMP
  • MT1-MMPMTMMP1
  • MT-MMP 1
  • MT-MMP1

Background

As the first member of membrane type (MT) MMPs, MMP-14, also known as MT1-MMP, plays an important role in extracellular matrix (ECM) remodeling by being able to degrade type I collagen, activate pro-MMP-2 and process cell adhesion molecules such as CD44 and Integrin alpha V (1). MMP-14 is therefore a key enzyme in many physiological and pathological processes such as angiogenesis and tumor invasion. Structurally, MMP-14 consists of the following domains: a pro domain containing the furin cleavage site, a catalytic domain containing the zinc-binding site, a hinge region, a hemopexin-like domain, a transmembrane domain, and a cytoplamasic tail (2).

  1. Seike, M. (2003) Cancer Lett. 194:1.
  2. Sato, H. et al. (1994) Nature 370:61.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

MMP-2 [HRP]
MMP200
N/A MMP-2 [HRP]
N/A MMP-2 [HRP]
(101 Publications)
Species: Ca, Hu, Mu, Po, Rt
Applications: ELISA
TIMP-2 [HRP]
DTM200
N/A TIMP-2 [HRP]
N/A TIMP-2 [HRP]
N/A TIMP-2 [HRP]
(36 Publications)
Species: Hu
Applications: ELISA
MMP-9 [HRP]
DMP900
N/A MMP-9 [HRP]
N/A MMP-9 [HRP]
(219 Publications)
Species: Hu
Applications: ELISA
TIMP-1 [HRP]
DTM100
N/A TIMP-1 [HRP]
N/A TIMP-1 [HRP]
(97 Publications)
Species: Hu
Applications: ELISA
MMP-13 [HRP]
DM1300
N/A MMP-13 [HRP]
N/A MMP-13 [HRP]
(33 Publications)
Species: Hu
Applications: ELISA
MMP-1 Antibody
(36665) [Unconjugated
MAB901
Knockout Validated MMP-1 Antibody (36665) [Unconjugated]
Immunohistochemistry MMP-1 Antibody (36665) [Unconjugated]
Immunohistochemistry MMP-1 Antibody (36665) [Unconjugated]
(40 Publications)
Species: Hu
Applications: IHC, IP, KO, Neut, WB
MMP-3 [HRP]
DMP300
N/A MMP-3 [HRP]
N/A MMP-3 [HRP]
(53 Publications)
Species: Hu
Applications: ELISA
MMP-15/MT2-MMP
Antibody (130522) [Un ...
MAB9161
MMP-15/MT2-MMP Antibody (130522) [Unconjugated]
(3 Publications)
Species: Hu
Applications: CyTOF-ready, IHC, ICFlow, WB
MMP-16/MT3-MMP
Antibody [Unconjugate
AF1785
Immunohistochemistry MMP-16/MT3-MMP Antibody [Unconjugated]
(1 Review)
Species: Hu
Applications: IHC, IP, WB
VEGF [HRP]
DVE00
N/A VEGF [HRP]
N/A VEGF [HRP]
N/A VEGF [HRP]
(680 Publications)
Species: Hu
Applications: ELISA
EMMPRIN/CD147
Antibody [Unconjugate
AF972
Western Blot EMMPRIN/CD147 Antibody [Unconjugated]
Immunohistochemistry EMMPRIN/CD147 Antibody [Unconjugated]
Knockout Validated EMMPRIN/CD147 Antibody [Unconjugated]
(1 Review)
(15 Publications)
Species: Hu
Applications: ELISA, IHC, KO, Simple Western, WB
MMP-7 [HRP]
DMP700
N/A MMP-7 [HRP]
N/A MMP-7 [HRP]
(38 Publications)
Species: Hu
Applications: ELISA
TNF-alpha
[Unconjugated]
210-TA
SDS-PAGE TNF-alpha [Unconjugated]
Bioactivity TNF-alpha [Unconjugated]
SEC-MALS TNF-alpha [Unconjugated]
(3 Reviews)
(809 Publications)
Species: Hu
Applications: BA
Metallothionein-1B
Overexpression Lysate ...
NBP2-10437
Western Blot Metallothionein-1B Overexpression Lysate
Species: Hu
Applications: WB
Melatonin
R1A/MT1/MTNR1A Antibo
NBP1-28912
Western Blot Melatonin R1A/MT1/MTNR1A Antibody
(5 Publications)
Species: Rt
Applications: PEP-ELISA, WB
Recombinant Human
Metallothionein-1M GS ...
H00004499-P01
SDS-PAGE Recombinant Human Metallothionein-1M GST (N-Term) Protein
Species: Hu
Applications: ELISA, AP, PA, PAGE, WB

Publications for MMP-14/MT1-MMP Antibody (MAB918)(3)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 2 applications: ICC, Western Blot.


Filter By Application
ICC
(2)
Western Blot
(1)
All Applications
Filter By Species
Human
(3)
All Species

Review for MMP-14/MT1-MMP Antibody (MAB918) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using MAB918:
Filter by Applications
IHC-P
(1)
All Applications
Filter by Species
Human
(1)
All Species
Images Ratings Applications Species Date Details
Immunohistochemistry-Paraffin MMP-14/MT1-MMP MAB918
Enlarge 		4
reviewed by:
Santhosh Sivajothi
 IHC-P Human 04/15/2019
View

Summary

ApplicationImmunohistochemistry-Paraffin
Sample TestedTonsil
SpeciesHuman
LotCFG0816111

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for MMP-14/MT1-MMP Antibody (MAB918) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Additional MMP-14/MT1-MMP Products

Blogs on MMP-14/MT1-MMP

There are no specific blogs for MMP-14/MT1-MMP, but you can read our latest blog posts.
Read our latest blog and use the new citation tool on bio-techne.com

Customers Who Bought This Also Bought

1 µg/lane of Recombinant Human TNF-alpha was resolved by SDS-PAGE with silver staining, under reducing (R) conditions, showing a band at 17 kDa.
TNF-alpha [Unconjugated]
210-TA-005

Contact Information

Product PDFs

  PDF SDS
  PDF Datasheet
  Request CofA

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Recent Reviews

4
5
0
4
1
3
0
2
0
1
0
Immunohistochemistry-Paraffin MMP-14/MT1-MMP MAB918
Enlarge
 Santhosh Sivajothi
04/15/2019
Application: IHC-P
Species: Human
read this review

Bioinformatics

Gene Symbol MMP14
Uniprot