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Porcine MMP-13 ELISA Kit (Colorimetric)

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Product Details

Summary
Reactivity PoSpecies Glossary
Applications ELISA
Suitable Sample Type
Serum, Plasma and other biological fluids
Standard Curve Range
62.5 - 4000 pg/mL
Sensitivity
26.1 pg/mL

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Porcine MMP-13 ELISA Kit (Colorimetric) Summary

Description
Assay time: 3 hours
Standard Curve Range
62.5 - 4000 pg/mL
Sensitivity
26.1 pg/mL
Assay Type
Sandwich ELISA
Inter-Assay
%CV < 12
Intra-Assay
%CV < 10
Spike Recovery
79 - 98%
Sample Volume
100 uL
Kit Type
ELISA Kit (Colorimetric)
Gene
MMP13

Applications/Dilutions

Dilutions
  • ELISA

Packaging, Storage & Formulations

Storage
Storage of components varies. See protocol for specific instructions.

Kit Components

Components
  1. Assay Diluent A
  2. Assay Diluent B
  3. Detection Reagent A
  4. Detection Reagent B
  5. Instruction manual
  6. Plate sealer for 96 wells
  7. Pre-coated ready to use 96-well strip plate
  8. Standard Diluent
  9. Standard
  10. Stop Solution
  11. TMB Substrate
  12. Wash Buffer (30 x concentrate)

Alternate Names for Porcine MMP-13 ELISA Kit (Colorimetric)

  • CLG 3
  • CLG3EC 3.4.24
  • collagenase 3
  • EC 3.4.24.-
  • EC 3.4.24.22
  • EC 3.4.24.24
  • EC 3.4.24.35
  • EC 3.4.24.65
  • EC 3.4.24.7
  • MANDP1
  • matrix metallopeptidase 13 (collagenase 3)
  • matrix metalloproteinase 13 (collagenase 3)
  • Matrix metalloproteinase-13
  • MMP13
  • MMP-13

Background

The matrix metalloproteinases (MMP) are a family of peptidase enzymes responsible for the degradation of extracellular matrix components, including collagen, gelatin, fibronectin, laminin and proteoglycan. Transcription of MMP genes is differentially activated by phorbol ester, lipopolysaccharide (LPS) or staphylococcal enterotoxin B (SEB). MMP catalysis requires both calcium and zinc. MMP-12 (also designated macrophage metalloelastase) is produced in alveolar macrophages and degrades elastin. MMP-12 may contribute to elastin degradation occurring in granulomatous skin diseases and may also participate in macrophage migration through the epidermal and vascular basement membranes in inflammatory disorders.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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Bioinformatics

Gene Symbol MMP13