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MKK6/MEK6 [p Ser207] Antibody

Images

 
Western Blot: MKK6/MEK6 [p Ser207] Antibody [NB100-82050] - Analysis of extracts from HL60 cells untreated or treated with PMA using MKK6(Phospho-Ser207) antibody
Immunocytochemistry/ Immunofluorescence: MKK6/MEK6 [p Ser207] Antibody [NB100-82050] - Staining of methanol-fixed Hela cells showing cytoplasmic staining using NB100-82050
Immunohistochemistry-Paraffin: MKK6/MEK6 [p Ser207] Antibody [NB100-82050] - Immunohistochemical analysis of paraffin- embedded human breast carcinoma tissue, using NB100-82050 (left) or the same antibody preincubated ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml

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MKK6/MEK6 [p Ser207] Antibody Summary

Immunogen
The antiserum was produced against synthesized phosphopeptide derived from human MKK6/MEK6 around the phosphorylation site of serine 207 (V-D-SP-V-A).
Modification
p Ser207
Specificity
Detects endogenous levels of MKK6/MEK6 only when phosphorylated at serine207.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
MAP2K6
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 1:50-200
  • Immunohistochemistry 1:100-1:300
  • Immunohistochemistry-Paraffin 1:50-1:100
  • Western Blot 1:500-1:2000
Theoretical MW
38 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control
MKK6/MEK6 Overexpression Lysate

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS, 50% glycerol, 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for MKK6/MEK6 [p Ser207] Antibody

  • MAP kinase kinase 6
  • MAP2K6
  • MAPK/ERK kinase 6
  • MAPKK6dual specificity mitogen-activated protein kinase kinase 6
  • MEK6
  • MEK6MAPKK 6
  • mitogen-activated protein kinase kinase 6
  • MKK6
  • MKK6MEK 6
  • PRKMK6
  • PRKMK6protein kinase, mitogen-activated, kinase 6 (MAP kinase kinase 6)
  • SAPKK3
  • SAPKK3EC 2.7.12.2

Background

Mitogen activated protein kinase kinase 6 (MEK6 or MKK6) belongs to the serine/threonine protein kinase family and the MAPK kinase subfamily (MAP2K, MKK or MEKs). MEK6, closely related to MEK3, catalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in MAP kinase p38, thus activating it, in response to inflammatory cytokines and environmental stress.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for MKK6/MEK6 Antibody (NB100-82050) (0)

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Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol MAP2K6
Entrez
Uniprot