Immunogen | E. coli-derived recombinant human MKK6 Ser2-Asp334 Accession # P52564 |
Specificity | Detects human, mouse, and rat MKK6. |
Source | N/A |
Isotype | IgG |
Clonality | Polyclonal |
Host | Goat |
Gene | MAP2K6 |
Purity Statement | Antigen Affinity-purified |
Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Buffer | Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS. |
Preservative | No Preservative |
Concentration | LYOPH |
Reconstitution Instructions | Reconstitute at 0.2 mg/mL in sterile PBS. |
Mitogen-activated protein kinase kinase 6 (MKK6), also known as MEK6, and the closely related MKK3 are activated by pro-inflammatory cytokines and environmental stresses (1, 2). Both MKK3 and MKK6 are dual specificity protein kinases, phosphorylating and activating the p38 MAP kinases at Thr and Tyr positions within the phosphoacceptor sequence Thr-Gly-Tyr (3). Activation of MKK6 occurs through phosphorylation at Ser207 and Thr211 (4) by several upstream MAPK kinase kinases, including ASK1 and TAK1 (5, 6). Purification of E. coli-expressed wild-type active MKK6 capable of p38 alpha phosphorylation has been previously noted (7).
Secondary Antibodies |
Isotype Controls |
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