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MKK6/MEK6 Antibody [Unconjugated]

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Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line, C6 rat glioma cell line, and B16-F1 mouse melanoma cell line. PVDF membrane was probed with 0.1 µg/mL Goat Anti-Human/Mouse/Rat MKK6 Antigen ...read more
Genetic Strategies: Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and MKK6 knockout HeLa cell line (KO). PVDF membrane was probed with 0.2 µg/mL of Goat ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, KO
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH
Validated by:
     

Genetic Strategies

   

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MKK6/MEK6 Antibody [Unconjugated] Summary

Immunogen
E. coli-derived recombinant human MKK6
Ser2-Asp334
Accession # P52564
Specificity
Detects human, mouse, and rat MKK6.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
MAP2K6
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Knockout Validated
  • Western Blot 0.1 ug/mL

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

The purchase of this product conveys to the buyer the limited, non-exclusive, non-transferable right (without the right to resell, repackage, or further sublicense) to use these reagents for research purposes only. No other license is granted to the buyer whether expressly, by implication, by estoppel or otherwise. In particular, the purchase of this product does not include nor carry any right or license to use, develop, or otherwise exploit this product commercially, which includes without limitation, provision of services to a third party, generation of commercial databases, clinical diagnostics or therapeutics, or drug development. This product is sold pursuant to a license from the University of Massachusetts, and the University of Massachusetts reserves all other rights to use these reagents. For information on purchasing a license for uses other than research, please contact the University of Massachusetts, Office of Technology Management, 333 South Street, Suite 400, Shrewsbury, Massachusetts, 01545.



This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for MKK6/MEK6 Antibody [Unconjugated]

  • MAP kinase kinase 6
  • MAP2K6
  • MAPK/ERK kinase 6
  • MAPKK6dual specificity mitogen-activated protein kinase kinase 6
  • MEK6
  • MEK6MAPKK 6
  • mitogen-activated protein kinase kinase 6
  • MKK6
  • MKK6MEK 6
  • PRKMK6
  • PRKMK6protein kinase, mitogen-activated, kinase 6 (MAP kinase kinase 6)
  • SAPKK3
  • SAPKK3EC 2.7.12.2

Background

Mitogen-activated protein kinase kinase 6 (MKK6), also known as MEK6, and the closely related MKK3 are activated by pro-inflammatory cytokines and environmental stresses (1, 2). Both MKK3 and MKK6 are dual specificity protein kinases, phosphorylating and activating the p38 MAP kinases at Thr and Tyr positions within the phosphoacceptor sequence Thr-Gly-Tyr (3). Activation of MKK6 occurs through phosphorylation at Ser207 and Thr211 (4) by several upstream MAPK kinase kinases, including ASK1 and TAK1 (5, 6). Purification of E. coli-expressed wild-type active MKK6 capable of p38 alpha phosphorylation has been previously noted (7).

  1. Goedert, M. et al. (1997) EMBO J. 16:3563.
  2. Moriguchi, T. et al. (1996) J. Biol. Chem. 271:26981.
  3. Raingeaud, J. et al. (1995) J. Biol. Chem. 270:7420.
  4. Han, J. et al. (1996) J. Biol. Chem. 271:2886.
  5. Ichijo, H. et al. (1997) Science 275:90.
  6. Moriguchi, T. et al. (1996) J. Biol. Chem. 271:13675.
  7. Keesler, G. et al. (1998) Protein Expr. Purif. 14:221.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol MAP2K6
Uniprot