MESP2 Antibody

MESP2 is a gene that codes for a protein that is 397 amino acids long, weighs approximately 42 kDa, and functions as a transcription factor that plays a role in somitogenesis and the development of cardiac mesoderm as well as regulates the FGF signaling pathway. Current research is being done on diseases and disorders related to this gene including dysostosis, alagille syndrome, cadasil, and scoliosis.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Images	Ratings	Applications	Species	Date	Details
	1 reviewed by: Gael Simon	ICC	Mouse	08/27/2021	View Summary Application Immunocytochemistry Sample Tested differentiated ESC cultures,Mouse ESC derived cells Species Mouse Lot QC6104-40416 Comments Comments ***Bio-Techne Response: This review was submitted through the legacy Novus Innovators Program, reflecting a new species or application tested on a primary antibody.*** The protocol used is the following : Fixation for 30 min in PFA, 4%, RT 3 washes for 5 min in PBS, RT Permeabilization and blocking for 1 hour in PBS + 3% BSA + 0.1% Triton, 4°C Primary antibody reaction OverNight in PBS + 3% BSA + 0.1% Triton + primary AB, 4°C (Mesp2 at 1ug/ml) 3 washes for 5 min in PBS, RT Blocking for 1 hour in PBS + 3% BSA + 0.1% Triton, 4°C Secondary antibody reaction for 2h in PBS + 3% BSA + 0.1% Triton + 1/500 secondary AB (Dylight 650 Donkey anti Rabbit), 20°C 3 washes for 15 min in PBS, RT (Second wash with 5um Hoechst)

Array NBP1-80219

• MESP2 Antibodies
• MESP2 Lysate