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Mesothelin Antibody (CL13965)

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Analysis in human fallopian tube and liver tissues using NBP3-24591 antibody. Corresponding MSLN RNA-seq data are presented for the same tissues.
Orthogonal Strategies: Analysis in human fallopian tube and liver tissues using NBP3-24591 antibody. Corresponding MSLN RNA-seq data are presented for the same tissues.
Staining of human liver shows no positivity in hepatocytes as expected.
Staining of human ovarian cancer (high grade serous ovarian carcinoma) shows strong membranous and cytoplasmic positivity in tumor cells.
Staining of human ovarian cancer (low grade serous ovarian carcinoma) shows strong membranous positivity in tumor cells.
Staining of human skin shows no positivity in squamous epithelial cells as expected.
Staining of human fallopian tube shows strong membranous-cytoplasmic positivity in glandular cells.
Orthogonal Strategies: Western blot analysis in human cell lines HeLa and PC-3 using Anti-MSLN antibody. Corresponding MSLN RNA-seq data are presented for the same cell lines. Loading control: Anti-HSP90B1.
Staining of human kidney shows moderate membranous positivity in cells in glomeruli.
Staining of human ovarian cancer (papillary carcinoma) shows strong membranous positivity in tumor cells.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC
Clone
CL13965
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Validated by:
       

Orthogonal Strategies

  

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Mesothelin Antibody (CL13965) Summary

Immunogen
This antibody was designed to target the recombinant protein Mesothelin, with the precise immunogen sequence being withheld for proprietary reasons.
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Gene
MSLN
Purity
Protein A purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Immunohistochemistry 1:2500 - 1:5000
  • Immunohistochemistry-Paraffin 1:2500 - 1:5000
  • Western Blot 1ug/ml
Application Notes
For IHC-Paraffin, we recommend using Heat-Induced Epitope Retrieval (HIER) with a pH level of 6. This optimized retrieval method ensures the best results for your experiments.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS, pH 7.2, 40% glycerol
Preservative
0.02% Sodium Azide
Purity
Protein A purified

Alternate Names for Mesothelin Antibody (CL13965)

  • CAK1 antigen
  • CAK1
  • megakaryocyte potentiating factor
  • Mesothelin
  • MPF
  • MPFSMRP
  • MSLN
  • Pre-pro-megakaryocyte-potentiating factor
  • SMR
  • soluble MPF mesothelin related protein

Background

MSLN, also known as Mesothelin, has 4 isoforms, a 630 amino acid isoform that is 69 kDa, a 622 amino acid isoform that is 68 kDa, a 656 amino acid isoform that is 71 kDa, and a 621 amino acid isoform that is 68 kDa, expressed in lung, heart, placenta kidney, and overexpressed in mesotheliomas, ovarian cancers, and some squamous cell carcinomas. Membrane-anchored forms may play a role in cellular adhesion and megakaryocyte-potentiating factor (MPF) potentiates megakaryocyte colony formation in vitro. Current research is being performed on several diseases and disorders including squamous cell carcinoma, ovarian cancer, carcinoma, malignant biphasic mesothelioma, bile duct adenoma, malignant mesothelioma, malignant pleural mesothelioma, gastrointestinal stromal tumor, renal clear cell carcinoma, epithelioid sarcoma, benign mesothelioma, pancreatic ductal adenocarcinoma, asbestosis, pancreatic ductal carcinoma, thymic carcinoma, acute myeloid leukemia, epithelial ovarian cancer, myeloid leukemia, thoracic cancer, and pancreatitis. This protein has also shown an interaction with MUC16 and RSG1 in cell adhesion and pancreas development processes and pathways.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol MSLN