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MBP Antibody (2H9) - Azide and BSA Free

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Western Blot: MBP Antibody (2H9) - Azide and BSA Free [NBP2-80844] - Total human brain lysate was separated by SDS-PAGE on a 12% gel and transferred to PVDF membrane. The membrane was then probed with anti-MBP antibody ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, ELISA, Flow, ICC/IF, IHC, CyTOF-ready
Clone
2H9
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
Azide and BSA Free
Concentration
1 mg/ml

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MBP Antibody (2H9) - Azide and BSA Free Summary

Immunogen
Purified recombinant fragment of human Myelin Basic Protein expressed in E. coli. [UniProt# P02686]
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
MBP
Purity
Ammonium sulfate precipitation
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Applications/Dilutions

Dilutions
  • CyTOF-ready
  • ELISA 1:10000
  • Flow Cytometry 1:200-1:400
  • Immunocytochemistry/ Immunofluorescence 1:200-1:1000
  • Immunohistochemistry
  • Immunohistochemistry-Frozen reported by customer review
  • Immunohistochemistry-Paraffin
  • Western Blot 1:500-1:2000
Theoretical MW
33 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Mouse reactivity reported from a verified customer review.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
No Preservative
Concentration
1 mg/ml
Purity
Ammonium sulfate precipitation

Alternate Names for MBP Antibody (2H9) - Azide and BSA Free

  • Hmbpr
  • MBP
  • MGC99675
  • mld
  • Myelin A1 protein
  • myelin basic protein
  • Myelin membrane encephalitogenic protein
  • shi

Background

The protein encoded by the classic MBP gene is a major constituent of the myelin sheath of oligodendrocytes and Schwann cells in the nervous system. However, MBP-related transcripts are also present in the bone marrow and the immune system. These mRNAs arise from the long MBP gene (otherwise called "Golli-MBP") that contains 3 additional exons located upstream of the classic MBP exons. Alternative splicing from the Golli and the MBP transcription start sites gives rise to 2 sets of MBP-related transcripts and gene products. The Golli mRNAs contain 3 exons unique to Golli-MBP, spliced in-frame to 1 or more MBP exons. They encode hybrid proteins that have N-terminal Golli aa sequence linked to MBP aa sequence. The second family of transcripts contain only MBP exons and produce the well characterized myelin basic proteins. This complex gene structure is conserved among species suggesting that the MBP transcription unit is an integral part of the Golli transcription unit and that this arrangement is important for the function and/or regulation of these genes.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol MBP