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MARK2 Antibody

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Western Blot: MARK2 Antibody [NBP1-00214] - (0.3ug/ml) staining of Human Brain (Cerebellum) 35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Western Blot: MARK2 Antibody [NBP1-00214] - (0.3ug/ml) staining of Human Brain (Cerebellum) 35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Product Details

Summary
Reactivity Hu, Mu, Rt, BvSpecies Glossary
Applications WB, ELISA
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

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MARK2 Antibody Summary

Immunogen
Peptide with sequence C-QNGKDSTAPQR corresponding to internal region according to NP_001156768.1, NP_001156769.1.
Specificity
This antibody is expected to recognise isoform c (NP_004945.4), isoform e (NP_001156768.1) and isoform f (NP_001156769.1).
Predicted Species
Mouse (100%), Rat (100%), Bovine (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
MARK2
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Peptide ELISA Detection limit 1:16000
  • Western Blot 0.3 - 1 ug/ml
Application Notes
WB: Approx. 70 kDa band observed in human brain (cerebellum) lysates (calculated MW of 77.6 kDa band according to NP_004945.3).
Publications
Read Publication using NBP1-00214.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for MARK2 Antibody

  • EC 2.7.11
  • EC 2.7.11.1
  • ELKL motif kinase 1
  • ELKL motif kinase
  • EMK-1
  • EMK1PAR-1
  • MAP/microtubule affinity-regulating kinase 2MGC99619
  • PAR1 homolog
  • Par1b
  • Ser/Thr protein kinase PAR-1B
  • serine/threonine protein kinase EMK
  • serine/threonine-protein kinase MARK2

Background

EMK (ELKL Motif Kinase) is a small family of ser/thr protein kinases involved in the control of cell polarity, microtubule stability and cancer. Several cDNA clones have been isolated that encoded two isoforms of the human ser/thr protein kinase EMK1. These isoforms were characterized by the presence of a 162-bp alternative exon that gave rise to two forms, one containing the exon and the other one lacking it. Both forms were found to be coexpressed in a number of selected cell lines and tissue samples. The human EMK1 was shown to be encoded by a single mRNA ubiquitously expressed. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for MARK2 Antibody (NBP1-00214)(1)


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Bioinformatics

Gene Symbol MARK2
Entrez
Uniprot