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Porcine Lymphotoxin-alpha/TNF-beta ELISA Kit (Colorimetric)

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Product Details

Summary
Reactivity PoSpecies Glossary
Applications ELISA
Suitable Sample Type
Serum, Plasma, Tissue homogenates, Cell lysates, Cell culture supernates and other biological fluids
Standard Curve Range
15.6 - 1000 pg/mL
Sensitivity
6.1 pg/mL

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Porcine Lymphotoxin-alpha/TNF-beta ELISA Kit (Colorimetric) Summary

Description
Assay time: 3 hours
Standard Curve Range
15.6 - 1000 pg/mL
Sensitivity
6.1 pg/mL
Assay Type
Sandwich ELISA
Inter-Assay
%CV < 12
Intra-Assay
%CV < 10
Spike Recovery
86 - 100%
Sample Volume
100 uL
Kit Type
ELISA Kit (Colorimetric)
Gene
LTA

Applications/Dilutions

Dilutions
  • ELISA

Packaging, Storage & Formulations

Storage
Storage of components varies. See protocol for specific instructions.

Kit Components

Components
  1. Assay Diluent A
  2. Assay Diluent B
  3. Detection Reagent A
  4. Detection Reagent B
  5. Instruction manual
  6. Plate sealer for 96 wells
  7. Pre-coated ready to use 96-well strip plate
  8. Standard Diluent
  9. Standard
  10. Stop Solution
  11. TMB Substrate
  12. Wash Buffer (30 x concentrate)

Alternate Names for Porcine Lymphotoxin-alpha/TNF-beta ELISA Kit (Colorimetric)

  • LT
  • LTA
  • LT-alpha
  • lymphotoxin alpha (TNF superfamily, member 1)
  • Lymphotoxin alpha
  • Lymphotoxinalpha
  • Lymphotoxin-alpha
  • tnfb
  • TNF-beta
  • TNFBlymphotoxin-alpha
  • TNFSF1
  • TNFSF1B
  • TNFSF1TNF-beta
  • tumor necrosis factor beta
  • Tumor necrosis factor ligand superfamily member 1

Background

TNF-beta (tumor necrosis factor beta, lymphotoxin alpha, TNFSF1, TNF ligand superfamily member 1) a member of the TNF ligand superfamily and a potent lymphoid factor, is mainly produced by activated T and B lymphocytes and leukocytes. TNF-beta is also produced by fibroblasts, astrocytes, myeloma cells, endothelial cells and epithelial cells. TNF-beta mediates inflammatory and immune responses, has cytotoxic activity on tumor cells and other target cells and shares many of the same bioactivities as TNF alpha, including regulation of cell proliferation, differentiation and apoptosis. TNF-beta is not species-specific and human TNF-beta is active on murine cells with a slightly reduced specific activity.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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Bioinformatics

Gene Symbol LTA