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Mouse LYAG/GAA ELISA Kit (Colorimetric)

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ELISA: Mouse LYAG/GAA ELISA Kit (Colorimetric) [NBP2-82399] - Samples were spiked with high concentrations of Mouse LYAG/GAA and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.
ELISA: Mouse LYAG/GAA ELISA Kit (Colorimetric) [NBP2-82399] - Samples were spiked with high concentrations of Mouse LYAG/GAA and diluted with Reference Standard & Sample Diluent to produce samples with values within the ...read more
ELISA: Mouse LYAG/GAA ELISA Kit (Colorimetric) [NBP2-82399] - Standard Curve Reference

Product Details

Summary
Reactivity MuSpecies Glossary
Applications ELISA
Suitable Sample Type
Serum, plasma, cell supernatant, and other biological fluids
Standard Curve Range
31.25 - 2000 pg/mL
Sensitivity
18.75 pg/mL

Order Details

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Mouse LYAG/GAA ELISA Kit (Colorimetric) Summary

Specificity
This kit recognizes Mouse LYAG/GAA in samples. No Significant cross-reactivity or interference between Mouse LYAG/GAA and analogues was observed.
Standard Curve Range
31.25 - 2000 pg/mL
Sensitivity
18.75 pg/mL
Assay Type
Sandwich-ELISA
Inter-Assay
CV% < 5.32
Intra-Assay
CV% < 5.59
Spike Recovery
84-103%
Sample Volume
100 ul
Kit Type
ELISA Kit (Colorimetric)
Gene
GAA

Applications/Dilutions

Dilutions
  • ELISA

Packaging, Storage & Formulations

Storage
Storage of components varies. See protocol for specific instructions.

Kit Components

Components
  1. Biotinylated Detection Ab Diluent
  2. Concentrated Biotinylated Detection Ab (100x)
  3. Concentrated HRP Conjugate (100x)
  4. Concentrated Wash Buffer (25x)
  5. HRP Conjugate Diluent
  6. Micro ELISA Plate (Dismountable)
  7. Plate Sealer
  8. Product Manual
  9. Reference Standard
  10. Sample Diluent
  11. Stop Solution
  12. Substrate Reagent

Alternate Names for Mouse LYAG/GAA ELISA Kit (Colorimetric)

  • Acid alpha-Glucosidase
  • Acid Maltase
  • Aglucosidase alfa
  • EC 3.2.1.20
  • GAA
  • glucosidase, alpha; acid
  • LYAG
  • Lysosomal alphaGlucosidase
  • Lysosomal alpha-Glucosidase

Background

GAA encodes acid alpha-glucosidase, which is essential for the degradation of glycogen to glucose in lysosomes. Different forms of acid alpha-glucosidase are obtained by proteolytic processing. Defects in this gene are the cause of glycogen storage disease II, also known as Pompe's disease, which is an autosomal recessive disorder with a broad clinical spectrum. Three transcript variants encoding the same protein have been found for this gene. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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  Mouse LYAG/GAA ELISA Kit (Colorimetric) Manual
  PDF Datasheet
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Bioinformatics

Gene Symbol GAA