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Lewis A Blood Group Antigen Antibody (SPM522) [CoraFluor™ 1]

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Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications Flow, ICC/IF, IHC, ICC/IF
Clone
SPM522
Clonality
Monoclonal
Host
Mouse
Conjugate
CoraFluor 1

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Lewis A Blood Group Antigen Antibody (SPM522) [CoraFluor™ 1] Summary

Description
CoraFluor(TM) 1 is a high performance terbium-based TR-FRET (Time-Resolved Fluorescence Resonance Energy Transfer) or TRF (Time-Resolved Fluorescence) donor for high throughput assay development. CoraFluor(IM) 1 absorbs UV light at approximately 340 nm, and emits at approximately 490 nm, 545 nm, 585 nm and 620 nm. It is compatible with common acceptor dyes that absorb at the emission wavelengths of CoraFluor(TM) 1. CoraFluor(TM) 1 can be used for the development of robust and scalable TR-FRET binding assays such as target engagement, ternary complex, protein-protein interaction and protein quantification assays.
Immunogen
Mucins isolated from ovarian cyst fluid
Localization
Cell membrane, cytoplasmic and extracellular
Specificity
Recognizes a carbohydrate determinant of Gal 1-3(Fuc 1-4) GlcNAc which is blood group antigen Lewis A. It is present primarily on epithelial cells such as colon and kidneys. In the tumors and dedifferentiated tissues, decrease of Lewis A antigen was observed. Lewis A (type 1 chain) is expressed in colonic epithelial cells and may be useful for detection of gastrointestinal tumors, pancreatic cancer, and colorectal tumors. Blood group related antigens represent a group of carbohydrate determinants carried on both glycolipids and glycoproteins. They are usually mucin-type, and are detected on erythrocytes, certain epithelial cells, and in secretions of certain individuals. Sixteen genetically and biosynthetically distinct but inter-related specificities belong to this group of antigens, including A, B, H, Lewis A, Lewis B, Lewis X, Lewis Y, and precursor type 1 chain antigens.
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
ABO
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry
  • Immunocytochemistry/ Immunofluorescence
  • Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark. Do not freeze.
Buffer
PBS
Preservative
No Preservative
Purity
Protein A or G purified

Notes

CoraFluor (TM) is a trademark of Bio-Techne Corp. Sold for research purposes only under agreement from Massachusetts General Hospital. US patent 2022/0025254

Alternate Names for Lewis A Blood Group Antigen Antibody (SPM522) [CoraFluor™ 1]

  • A transferase
  • A3GALNT
  • A3GALT1
  • ABO
  • B transferase
  • EC=2.4.1.37
  • EC=2.4.1.40
  • Fucosylglycoprotein 3-alpha-galactosyltransferase
  • Fucosylglycoprotein alpha-N-acetylgalactosaminyltransferase soluble form
  • Fucosylglycoprotein alpha-N-acetylgalactosaminyltransferase
  • Glycoprotein-fucosylgalactoside alpha-galactosyltransferase
  • Glycoprotein-fucosylgalactoside alpha-N-acetylgalactosaminyltransferase
  • GTB
  • Histo-blood group A transferase
  • Histo-blood group ABO system transferase
  • Histo-blood group B transferase
  • NAGAT

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol ABO