![Western Blot: KLH Antibody [Biotin] [NBP1-97337] - Lane 1: KLH reduced. Lane 2: none. Load: 1 ug per lane. Primary antibody: KLH antibody at 1:1000 for overnight at 4C. Secondary antibody: HRP Goat-anti Rabbit secondary antibody at 1:40,000 for 45 min at RT. Block: 1% BSA-TTBS for 30 min RT. Predicted/Observed size: 350-390 kDa for KLH. Other band(s): none.](http://images.novusbio.com/fullsize/KLH-Antibody-[Biotin]-Western-Blot-NBP1-97337-img0001.jpg "Western Blot: KLH Antibody [Biotin] [NBP1-97337] - Lane 1: KLH reduced. Lane 2: none. Load: 1 ug per lane. Primary antibody: KLH antibody at 1:1000 for overnight at 4C. Secondary antibody: HRP Goat-anti Rabbit secondary antibody at 1:40,000 for 45 min at RT. Block: 1% BSA-TTBS for 30 min RT. Predicted/Observed size: 350-390 kDa for KLH. Other band(s): none.")
| Reactivity | AllSpecies Glossary |
| Applications | WB, ELISA, IHC |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Biotin |
| Concentration | LYOPH |
| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| Alkaline Phosphatase | NBP1-97336 | |||
| FITC | NBP1-97356 | |||
| HRP | NBP1-97357 | |||
| Rhodamine | NBP1-97386 | |||
| Texas Red | NBP1-97387 | |||
| Unconjugated | NB120-7054 | |||
| Description | Store vial at 4C prior to restoration. For extended storage aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use. Stabilizer: 10 mg/ml Bovine Serum Albumin (BSA)-Immunoglobulin and Protease free Color: n/a Conjugation Chemistry: N-hydroxysuccinimide (NHS) ester This biotin conjugated antibody was prepared from monospecific antiserum by immunoaffinity chromatography using KLH coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum, anti-Biotin, and KLH. |
| Immunogen | KLH (Keyhole Limpet Hemocyanin) |
| Localization | Secreted protein; extracellular space |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
|
| Application Notes | This product has been tested by western blotting to detect a single band of the expected apparent molecular weight and for ELISA. Anti-KLH antibody biotin conjugate is suitable for use with streptavidin reporter systems. Researchers should determine optimal titers for applications that are not stated below. |
| Readout System |
| Storage | Store lyophilized antibody at 4C in the dark. Aliquot reconstituted liquid and store at -20C. Avoid freeze-thaw cycles. |
| Buffer | Lyophilized from 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free |
| Preservative | 0.01% Sodium Azide |
| Concentration | LYOPH |
| Purity | Immunogen affinity purified |
| Reconstitution Instructions | Reconstitute with 100 ul deionized water (or equivalent) |
Secondary Antibodies |
Isotype Controls |
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")