![Western Blot: KLH Antibody [Alkaline Phosphatase] [NBP1-97336] - Lane 1: molecular weight. Lane 2: reduced KLH. Load: 1 ug. Primary antibody: KLH antibody at 1:1000 for overnight at 4C. Predicted/Observed size: 350-390 kDa, 350-390 kDa for KLH. Other band(s): none.](http://images.novusbio.com/fullsize/KLH-Antibody-[Alkaline-Phosphatase]-Western-Blot-NBP1-97336-img0001.jpg "Western Blot: KLH Antibody [Alkaline Phosphatase] [NBP1-97336] - Lane 1: molecular weight. Lane 2: reduced KLH. Load: 1 ug. Primary antibody: KLH antibody at 1:1000 for overnight at 4C. Predicted/Observed size: 350-390 kDa, 350-390 kDa for KLH. Other band(s): none.")
| Reactivity | AllSpecies Glossary |
| Applications | WB, ELISA |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Alkaline Phosphatase |
| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| Biotin | NBP1-97337 | |||
| FITC | NBP1-97356 | |||
| HRP | NBP1-97357 | |||
| Rhodamine | NBP1-97386 | |||
| Texas Red | NBP1-97387 | |||
| Unconjugated | NB120-7054 | |||
| Description | Store vial at 4C before opening. DO NOT FREEZE. This product is stable at 4C as an undiluted liquid. Dilute only prior to immediate use. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity. Stabilizer: 10 mg/ml Bovine Serum Albumin (BSA)-Immunoglobulin and Protease free Color: Substrate dependent Conjugation Chemistry: Glutaraldehye coupling This product was prepared from monospecific antiserum by immunoaffinity chromatography using KLH coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase (calf intestine), anti-Rabbit Serum and KLH. |
| Immunogen | KLH (Keyhole Limpet Hemocyanin) |
| Localization | Secreted protein; extracellular space |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | This product has been assayed against 1.0 ug of KLH in a standard capture ELISA using pNPP p-nitrophenyl phosphate as a substrate for 30 minutes at room temperature. A working dilution of 1:3,000 to 1:13,500 is suggested for this product. |
| Storage | Store at 4C in the dark. Do not freeze. |
| Buffer | 0.05 M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50% (v/v) Glycerol; pH 8.0, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free |
| Preservative | 0.01% Sodium Azide |
| Purity | Immunogen affinity purified |
Secondary Antibodies |
Isotype Controls |
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![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
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