Kallikrein 13 Overexpression Lysate

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.

KLK13, also known as Kallikrein 13, is a 30,6 kDa 277 amino acid protein, expressed in prostate, breast, testis and salivary gland, belongs to the peptidase S1 family, kallikrein subfamily. Kallikreins are a subgroup of serine proteases having diverse physiological functions including their implication in carcinogenesis based on growing evidence. Disease research is currently being studied with relation to KLK13 and ovarian cancer, breast cancer, noma, testicular cancer, lung adenocarcinoma, prostatitis, prostate cancer, and carcinoma. Interactions with KLK13 protein have been shown to involve SERPINF2, SERPINA1, and A2M in proteolysis and protein processing pathways.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Array NBL1-12348

• Kallikrein 13 Antibodies
• Kallikrein 13 Antibody Pair
• Kallikrein 13 ELISA Kit
• Kallikrein 13 Lysate
• Kallikrein 13 Proteins and Enzyme