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ISCU Antibody (3B8-1C4)

Images

 
Western Blot: ISCU Antibody (3B8-1C4) [H00023479-M01] - NIFUN monoclonal antibody (M01), clone 3B8-1C4 Analysis of NIFUN expression in HL-60.
Immunocytochemistry/ Immunofluorescence: ISCU Antibody (3B8-1C4) [H00023479-M01] - Analysis of monoclonal antibody to NIFUN on HeLa cell. Antibody concentration 10 ug/ml.
Sandwich ELISA: ISCU Antibody (3B8-1C4) [H00023479-M01] - Detection limit for recombinant GST tagged NIFUN is approximately 0.03ng/ml as a capture antibody.

Product Details

Summary
Reactivity Hu, RtSpecies Glossary
Applications WB, ELISA, ICC/IF
Clone
3B8-1C4
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

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ISCU Antibody (3B8-1C4) Summary

Immunogen
NIFUN (AAH11906, 26 a.a. ~ 167 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. RELSAPARLYHKKVVDHYENPRNVGSLDKTSKNVGTGLVGAPACGDVMKLQIQVDEKGKIVDARFKTFGCGSAIASSSLATEWVKGKTVEEALTIKNTDIAKELCLPPVKLHCSMLAEDAIKAALADYKLKQEPKKGEAEKK
Specificity
NIFUN - NifU-like N-terminal domain containing
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
ISCU
Purity
IgG purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • ELISA
  • Immunocytochemistry/ Immunofluorescence
  • Sandwich ELISA
  • Western Blot 1:500
Application Notes
Antibody reactive against cell lysate and recombinant protein for Western Blot. Has also been used for immunofluoresence and ELISA.
Publications
Read Publication using H00023479-M01.

Reactivity Notes

Human. Other species not tested.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
In 1x PBS, pH 7.4
Preservative
No Preservative
Purity
IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for ISCU Antibody (3B8-1C4)

  • 2310020H20Rik
  • HML
  • hnifU
  • iron-sulfur cluster assembly enzyme ISCU, mitochondrial
  • iron-sulfur cluster scaffold homolog (E. coli)
  • IscU iron-sulfur cluster scaffold homolog (E. coli)
  • IscU iron-sulfur cluster scaffold homolog
  • IscU
  • ISU2
  • MGC74517
  • NIFU
  • NifU-like N-terminal domain containing
  • NifU-like N-terminal domain-containing protein
  • NifU-like protein
  • NIFUN

Background

Iron-sulfur (Fe-S) clusters are necessary for several mitochondrial enzymes and other subcellular compartment proteins. They contain sulfur and iron, and are created via several steps that include cysteine desulfurases, iron donors, chaperones, and scaffold proteins. This gene encodes the two isomeric forms, ISCU1 and ISCU2, of the Fe-S cluster scaffold protein. Mutations in this gene have been found in patients with myopathy with severe exercise intolerance and myoglobinuria.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for ISCU Antibody (H00023479-M01)(1)


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Product General Protocols

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WB Video Protocol
ICC/IF Video Protocol

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Bioinformatics

Gene Symbol ISCU
Entrez
Uniprot