![Western Blot: IRX6 Antibody (1A5) [H00079190-M01] - Analysis of IRX6 expression in transfected 293T cell line by IRX6 monoclonal antibody (M01), clone 1A5.Lane 1: IRX6 transfected lysate(48.169 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/IRX6-Antibody-1A5-Western-Blot-H00079190-M01-img0005.jpg "Western Blot: IRX6 Antibody (1A5) [H00079190-M01] - Analysis of IRX6 expression in transfected 293T cell line by IRX6 monoclonal antibody (M01), clone 1A5.Lane 1: IRX6 transfected lysate(48.169 KDa).Lane 2: Non-transfected lysate.")
| Reactivity | HuSpecies Glossary |
| Applications | WB, ELISA, KD |
| Clone | 1A5 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Immunogen | IRX6 (NP_077311, 337 a.a. ~ 446 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. PRLTMHYPCLEKPRIWSLAHTATASAVEGAPPARPRPRSPECRMIPGQPPASARRLSVPRDSACDESSCIPKAFGNPKFALQGLPLNCAPCPRRSEPVVQCQYPSGAEAG |
| Specificity | IRX6 (1A5) |
| Isotype | IgG1 Kappa |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | IRX6 |
| Purity | IgG purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | Antibody reactive against transfected lysate and recombinant protein for Western Blot. It has also been used for ELISA and RNAi validation. |
| Storage | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Buffer | In 1x PBS, pH 7.4 |
| Preservative | No Preservative |
| Purity | IgG purified |
![Western Blot RFC1 Antibody [Unconjugated]](https://images.novusbio.com/images2/RFC1_AF6457_Western_Blot_9874.jpg)
Secondary Antibodies |
Isotype Controls |
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![Western Blot: IRX6 Antibody (1A5) [H00079190-M01] - Analysis of IRX6 over-expressed 293 cell line, cotransfected with IRX6 Validated Chimera RNAi ( Cat # H00079190-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with IRX6 monoclonal antibody (M01), clone 1A5 (Cat # H00079190-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.](http://images.novusbio.com/fullsize/IRX6-Antibody-1A5-Western-Blot-H00079190-M01-img0003.jpg "Western Blot: IRX6 Antibody (1A5) [H00079190-M01] - Analysis of IRX6 over-expressed 293 cell line, cotransfected with IRX6 Validated Chimera RNAi ( Cat # H00079190-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with IRX6 monoclonal antibody (M01), clone 1A5 (Cat # H00079190-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.")
![Sandwich ELISA: IRX6 Antibody (1A5) [H00079190-M01] - Detection limit for recombinant GST tagged IRX6 is 0.1 ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/IRX6-Antibody-1A5-Sandwich-ELISA-H00079190-M01-img0004.jpg "Sandwich ELISA: IRX6 Antibody (1A5) [H00079190-M01] - Detection limit for recombinant GST tagged IRX6 is 0.1 ng/ml as a capture antibody.")
![Immunocytochemistry Aldehyde Dehydrogenase 1-A1/ALDH1A1 Antibody [Unconjugated]](https://images.novusbio.com/images2/Aldehyde_Dehydrogenase_1-A1_AF5869_Immunocytochemistry_10308.jpg)
![Immunohistochemistry Aldehyde Dehydrogenase 1-A1/ALDH1A1 Antibody [Unconjugated]](https://images.novusbio.com/images2/Aldehyde_Dehydrogenase_1-A1_AF5869_Immunohistochemistry_10638.jpg)
![Simple Western Aldehyde Dehydrogenase 1-A1/ALDH1A1 Antibody [Unconjugated]](https://images.novusbio.com/images2/Aldehyde_Dehydrogenase_1A1_AF5869_Simple_Western_17067.jpg)
![Bioactivity IL-2 [Unconjugated]](https://images.novusbio.com/images/202-il_recombinant-human-il-2-protein-bioactivity-174202314946.jpg)
![Flow Cytometry: Mouse IgG1 Kappa Isotype Control (P3.6.2.8.1) [NBP1-43319] - Analysis of Alexa Fluor (R) 700 conjugate of NBP1-43319. Human buffy coat stained with mouse IgG1 Kappa isotype control. Flow cytometry image submitted by a verified customer review.](https://images.novusbio.com/images/Mouse-IgG1-Kappa-Light-Chain-Isotype-Control-P3-6-2-8-1-Flow-Cytometry-NBP1-43319-img0002.jpg "Flow Cytometry: Mouse IgG1 Kappa Isotype Control (P3.6.2.8.1) [NBP1-43319] - Analysis of Alexa Fluor (R) 700 conjugate of NBP1-43319. Human buffy coat stained with mouse IgG1 Kappa isotype control. Flow cytometry image submitted by a verified customer review.")