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IRF2 Antibody

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Western Blot: IRF2 Antibody [NB100-1089] - Staining (2ug/ml) of Jurkat lysate (RIPA buffer, 30ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
Western Blot: IRF2 Antibody [NB100-1089] - Staining (2ug/ml) of Jurkat lysate (RIPA buffer, 30ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

Product Details

Summary
Reactivity Hu, Mu, Rt, Bv, CaSpecies Glossary
Applications WB, ELISA
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

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IRF2 Antibody Summary

Immunogen
Peptide with sequence KTSDITQARVKSC corresponding to C-Terminus according to NP_002190.2.
Predicted Species
Rat (100%), Canine (100%), Bovine (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
IRF2
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Peptide ELISA Detection limit 1:2000
  • Western Blot 1 - 3 ug/ml
Application Notes
Approx 48kDa band observed in lysates of cell line Jurkat (calculated MW of 39.3kDa according to NP 002190.2). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Li et al, Invest Ophthalmol Vis Sci. 1999 Apr, 40(5):976-82., PMID: 10102295. Recommended concentration: 1-3ug/ml.
Control
Jurkat Whole Cell Lysate
IRF2 Overexpression Lysate
Publications
Read Publication using NB100-1089.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for IRF2 Antibody

  • DKFZp686F0244
  • interferon regulatory factor 2
  • IRF2
  • IRF-2

Background

IRF2 encodes interferon regulatory factor 2, a member of the interferon regulatory transcription factor (IRF) family. IRF2 competitively inhibits the IRF1-mediated transcriptional activation of interferons alpha and beta, and presumably other genes that employ IRF1 for transcription activation. However, IRF2 also functions as a transcriptional activator of histone H4.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for IRF2 Antibody (NB100-1089)(1)


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Product General Protocols

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Video Protocols

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FAQs for IRF2 Antibody (NB100-1089) (0)

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Control Lysate(s)

Jurkat Whole Cell Lysate
IRF2 Overexpression Lysate

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol IRF2
Entrez
Uniprot