Rat Insulin Receptor beta ELISA Kit (Chemiluminescence) Summary
Specificity |
This kit recognizes Rat INSR-Beta in samples. No significant cross-reactivity or interference between Rat INSR-Beta and analogues was observed. |
Standard Curve Range |
0.16-10 ng/mL |
Sensitivity |
0.09 ng/mL |
Assay Type |
Sandwich-CLIA |
Inter-Assay |
CV% < 9.46% |
Intra-Assay |
CV% < 10.09% |
Spike Recovery |
91-114% |
Sample Volume |
100 uL |
Kit Type |
ELISA Kit (Chemiluminescence) |
Gene |
INSR |
Applications/Dilutions
Packaging, Storage & Formulations
Storage |
Storage of components varies. See protocol for specific instructions. |
Kit Components
Components
|
- Biotinylated Detection Ab Diluent
- Concentrated Biotinylated Detection Ab (100x)
- Concentrated HRP Conjugate (100x)
- Concentrated Wash Buffer (25x)
- HRP Conjugate Diluent
- Micro CLIA Plate (Dismountable)
- Plate Sealer
- Product Manual
- Reference Standard
- Sample Diluent
- Substrate Reagent A
- Substrate Reagent B
|
Alternate Names for Rat Insulin Receptor beta ELISA Kit (Chemiluminescence)
Background
The Insulin Receptor (InsR) is a heterodimeric receptor tyrosine kinase with an extracellular alpha-chain, a transmembrane domain and an intracellular beta-chain. InsR is activated upon binding of the peptide hormone insulin, leading to autophosphorylation of tyrosine residues 1146, 1150, and 1151 in the activation loop of the beta-chain. Additional autophosphorylation sites such as tyrosine residues 960, 972, 1316, and 1322 regulate the assembly of signal transduction complexes.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are
guaranteed for 6 months from date of receipt.
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