![Western Blot: Influenza A H1N1 Antibody - (A/California/14/2009), [NBP1-30320] - For the validation Influenza A Virus antibody in WB Assay, 3 ug of intact A/California/14/2009 H1N1 virions were loaded on SDS-PAGE, transferred to a PVDF membrane and detected with NBP1-30320 antibody at a dilution of 1:1000. Goat anti-rabbit secondary antibody was used at 1:10,000 dilution. NBP1-30320 detects: Hemagglutinin (HA) ~75 kDa, Neuraminidase monomer (NA) ~55 kDa, Matrix (M) ~26 kDa and Non-structural Protein monomer (NP) ~26-27 kDa (M and NP often co-migrate as 1 band).](http://images.novusbio.com/fullsize/Influenza-A-Virus-Antibody-Western-Blot-NBP1-30320-img0001.jpg "Western Blot: Influenza A H1N1 Antibody - (A/California/14/2009), [NBP1-30320] - For the validation Influenza A Virus antibody in WB Assay, 3 ug of intact A/California/14/2009 H1N1 virions were loaded on SDS-PAGE, transferred to a PVDF membrane and detected with NBP1-30320 antibody at a dilution of 1:1000. Goat anti-rabbit secondary antibody was used at 1:10,000 dilution. NBP1-30320 detects: Hemagglutinin (HA) ~75 kDa, Neuraminidase monomer (NA) ~55 kDa, Matrix (M) ~26 kDa and Non-structural Protein monomer (NP) ~26-27 kDa (M and NP often co-migrate as 1 band).")
| Reactivity | V-ViSpecies Glossary |
| Applications | WB |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Format | BSA Free |
| Concentration | 1 mg/ml |
| Immunogen | A/California/14/2009 (H1N1) virions were used as an immunogen. |
| Specificity | This antibody detects the following H1N1 proteins: Hemagglutinin (HA) ~75 kDa, Neuraminidase monomer (NA) ~55 kDa, Matrix (M) ~26 kDa and Non-structural Protein monomer (NS) ~26-27 kDa. Reacts with A/New Caledonia/20/99, A/Brisbane/10/2007, A/California/14/2009 and likely reacts with most other isolates. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | HA |
| Purity | Protein G purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | Western Blot detection. |
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| Reviewed Applications |
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| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS |
| Preservative | 0.02% Sodium Azide |
| Concentration | 1 mg/ml |
| Purity | Protein G purified |
| Images | Ratings | Applications | Species | Date | Details | ||||||||
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reviewed by:
Mona Agrawal |
WB | Human and Other | 09/22/2023 |
Summary
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Secondary Antibodies |
Isotype Controls |
Research Areas for Influenza A H1N1 Antibody (NBP1-30320)Find related products by research area.
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| Gene Symbol | HA |
![Western Blot: Rabbit Polyclonal Influenza A H1N1 Antibody [IMGENEX: IMG-4993] [NBP1-30320] - Image of IAV infected and non-infected A549 whole cell lysates. Image from a verified customer review.](http://images.novusbio.com/fullsize/antibody/nbp1-30320_rabbit-polyclonal-influenza-a-h1n1-antibody-imgenex-img-4993-western-blot-61020231523..png "Western Blot: Rabbit Polyclonal Influenza A H1N1 Antibody [IMGENEX: IMG-4993] [NBP1-30320] - Image of IAV infected and non-infected A549 whole cell lysates. Image from a verified customer review.")
![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")
