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Human IL6R ELISA Kit (Colorimetric)

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Sandwich ELISA: Human IL6R ELISA Kit (Colorimetric) [NBP1-89870] - Representative standard curve for human IL-6R ELISA. Human IL-6R was diluted in serial 2-fold steps in IL-6RAssay Buffer (1x). Do not use this standard curve to derive test results. A standard curve must be run for each group ofmicrowell strips assayed.
Sandwich ELISA: Human IL6R ELISA Kit (Colorimetric) [NBP1-89870] - Representative standard curve for human IL-6R ELISA. Human IL-6R was diluted in serial 2-fold steps in IL-6RAssay Buffer (1x). Do not use this standard ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications ELISA
Suitable Sample Type
Cell culture supernatant, serum, plasma (EDTA, heparin), amniotic fluid, urine
Standard Curve Range
0.08 - 5.0 ng/mL
Sensitivity
0.01 ng/mL

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Human IL6R ELISA Kit (Colorimetric) Summary

Description
This kit is an enzyme-linked immunosorbent assay for quantitative detection.
Specificity
The interference of circulating factors of the immune systeme was evaluated by spiking these proteins at physiologically relevant concentrations into a human IL-6R positive serum. There was no crossreactivity detected.
Standard Curve Range
0.08 - 5.0 ng/mL
Sensitivity
0.01 ng/mL
Assay Type
Sandwich ELISA
Inter-Assay
CV% < 2.2%
Intra-Assay
CV% < 1.7%
Sample Volume
20 uL
Kit Type
ELISA Kit (Colorimetric)
Gene
IL6R

Applications/Dilutions

Dilutions
  • ELISA
Reviewed Applications
Read 1 Review rated 5
using
NBP1-89870 in the following application:
Publications
Read Publication using
NBP1-89870 in the following applications:

Packaging, Storage & Formulations

Storage
Storage of components varies. See protocol for specific instructions.

Kit Components

Components
  1. Adhesive Plate Covers
  2. Aluminium pouch(es) with a human sIL-6R Standard curve (coloured)
  3. Aluminium pouch(es) with a Microwell Plate coated with Monoclonal Antibody (murine) to human sIL-6R and HRP-Conjugate (anti-sIL-6R monoclonal (murine) antibody), lyophilized
  4. Assay Buffer Concentrate 20x (PBS with 1% Tween 20 and 10% BSA)
  5. Lyophilized Control
  6. Stop Solution (1M Phosphoric acid)
  7. Substrate Solution (tetramethyl-benzidine)
  8. Wash Buffer Concentrate 20x (phosphate-buffered saline with 1% Tween 20)

Alternate Names for Human IL6R ELISA Kit (Colorimetric)

  • CD126 antigen
  • CD126
  • gp80
  • IL-6 receptor subunit alpha
  • IL-6R 1
  • IL-6R subunit alpha
  • IL-6R-1
  • IL6RA
  • IL-6RA
  • IL-6R-alpha
  • interleukin 6 receptor alpha subunit
  • interleukin 6 receptor
  • interleukin-6 receptor subunit alpha
  • Membrane glycoprotein 80
  • MGC104991

Background

Interleukin 6 (IL6) is a potent pleiotropic cytokine that regulates cell growth and differentiation and plays an important role in immune response. The protein encoded by this gene is a subunit of the receptor complex for IL6. The IL6 receptor is a protein complex consisting of this protein and interleukin 6 signal transducer (IL6ST/GP130/IL6-beta), a receptor subunit also shared by many other cytokines. Dysregulated production of IL6 and this receptor are implicated in the pathogenesis of many diseases, such as multiple myeloma, autoimmune diseases and prostate cancer. Alternatively spliced transcript variants encoding distinct isoforms have been reported. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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Publications for IL6R ELISA Kit (NBP1-89870)(1)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: ELISA.


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Review for IL6R ELISA Kit (NBP1-89870) (1) 51

Average Rating: 5
(Based on 1 review)

Reviews using NBP1-89870:
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  5
reviewed by:
Verified Customer
 05/25/2017
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Summary

Sample TestedHuman post-MI plasma samples

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Contact Information

Product PDFs

  SDS
  Manual - Human IL6R ELISA Kit (Colorimetric)
  PDF Datasheet
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Recent Reviews

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Species:
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Bioinformatics

Gene Symbol IL6R