Is the Recombinant Canine Il-31 Protein (NBP2-59591) functional? As in, could it be used to stimulate canine cells in an assay?

Name: Recombinant Canine IL-31 His Protein
Brand: Novus Biologicals
SKU: NBP2-59591
Rating: 5 (1 reviews)

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Accepted Answer

Unfortunately, this canine Il-31 protein was manufactured for the purpose of a control for SDS-PAGE and possibly for western blot. It has not been tested for bioactivity.

At	=	A. thaliana	SyHa	=	Golden Syrian Hamster
All	=	All Species	Ha	=	Hamster
An	=	Animal	Hu	=	Human
ArHa	=	Armenian Hamster	I	=	Insect
Av	=	Avian	Ll	=	Llama
Bb	=	Baboon	Ma	=	Mammal
Ba	=	Bacteria	Mk	=	Monkey
Bv	=	Bovine	Mu	=	Mouse
Ca	=	Canine	Multi	=	Multi-species
Ce	=	C. Elegans	NA	=	Non-species specific
Ch	=	Chicken	Or	=	Orangutan
ChHa	=	Chinese Hamster	Pl	=	Plant
Cm	=	Cynomolgus Monkey	Pm	=	Primate
Do	=	Donkey	Po	=	Porcine
Dr	=	Drosophilia	Rb	=	Rabbit
Ec	=	E. Coli	Rt	=	Rat
Eq	=	Equine	RM	=	Rhesus Macaque
Fe	=	Feline	Sh	=	Sheep
Ft	=	Ferret	Vb	=	Vertebrate
Fi	=	Fish	Vi	=	Virus
Fu	=	Fungi	Xp	=	Xenopus
Ge	=	Gerbil	Ye	=	Yeast
GP	=	Guinea Pig	Ze	=	Zebrafish
Gt	=	Goat

Reactivity	CaSpecies Glossary
Applications	PAGE
Concentration	1 mg/ml

Description	A recombinant protein with a C-Terminal His-tag and corresponding to the amino acids 24-159 of Canine IL-31 Source:Baculovirus Amino Acid Sequence: SHMAPTHQLP PSDVRKIILE LQPLSRGLLE DYQKKETGVP ESNRTLLLCL TSDSQPPRLN SSAILPYFRA IRPLSDKNII DKIIEQLDKL KFQHEPETEI SVPADTFECK SFILTILQQF SACLESVFKS LNSGPQHHHH HH
Source	Baculovirus
Protein/Peptide Type	Recombinant Protein
Gene	IL31
Purity	>95%, by SDS-PAGE
Endotoxin Note	< 1.0 EU per 1 microgram of protein (determined by LAL method)

Storage	Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer	PBS (pH 7.4), 10% glycerol
Preservative	No Preservative
Concentration	1 mg/ml
Purity	>95%, by SDS-PAGE

Publications using NBP2-59591	Applications	Species
Massimini M, Dalle Vedove E, Bachetti B et al. Polyphenols and Cannabidiol Modulate Transcriptional Regulation of Th1/Th2 Inflammatory Genes Related to Canine Atopic Dermatitis Frontiers in veterinary science 2021-03-05 [PMID: 33763461] (Canine)		Canine
Wheeler JJ, Lascelles BD, Olivry T et al. Itch-associated Neuropeptides and Their Receptor Expression in Dog Dorsal Root Ganglia and Spinal Cord. Acta Dermato-Venereologica 2019-11-01 [PMID: 31449313] (ELISA, ELISA)	ELISA, ELISA

Recombinant Canine IL-31 His Protein

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	Verified Customer 10/02/2020
Application:	Immunoassay Standard
Species:

Gene Symbol	IL31
Entrez	100302725(Canine)