Home » IL-1ra/IL-1F3/IL1RN » IL-1ra/IL-1F3/IL1RN Antibodies » IL-1ra/IL-1F3/IL1RN Antibody [Biotin]
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IL-1ra/IL-1F3/IL1RN Antibody [Biotin]

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Product Details

Summary
Reactivity MuSpecies Glossary
Applications WB, ELISA(Det)
Clonality
Polyclonal
Host
Goat
Conjugate
Biotin
Concentration
LYOPH

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Unconjugated
AF-480-NA
View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
AF-480-NA
Carrier Free
AF-480-NA
Carrier Free
AF-480-SP
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IL-1ra/IL-1F3/IL1RN Antibody [Biotin] Summary

Immunogen
E. coli-derived recombinant mouse IL‑1ra/IL-1F3
Specificity
Detects mouse IL‑1ra/IL-1F3 in ELISAs and Western blots. In sandwich immunoassays, approximately 45% cross-reactivity with recombinant rat IL‑1ra is observed and less than 1% cross-reactivity with recombinant human IL-1ra, recombinant porcine IL-1ra, and recombinant equine IL‑1ra is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
IL1RN
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
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Applications/Dilutions

Dilutions
  • ELISA Capture (Matched Antibody Pair) 0.2-0.8 ug/mL
  • ELISA Detection (Matched Antibody Pair) 0.1-0.4 ug/mL
  • ELISA Standard (Matched Pair)
  • Western Blot 0.1 ug/mL
Application Notes
ELISA Capture: Mouse IL-1ra/IL-1F3 Antibody (Catalog # AF-480-NA)
ELISA Detection: Mouse IL-1ra/IL-1F3 Biotinylated Antibody (Catalog # BAF480)
Standard: Recombinant Mouse IL-1ra/IL-1F3 (Catalog # 480-RM)
Readout System
Publications
Read Publications using
BAF480 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for IL-1ra/IL-1F3/IL1RN Antibody [Biotin]

  • DIRA
  • ICIL-1ra
  • IL1F3
  • IL-1F3
  • IL1ra
  • IL-1ra
  • IL-1ra3
  • IL1RN
  • IL-1RN
  • interleukin 1 receptor antagonist
  • IRAP
  • MVCD4

Background

IL-1ra was originally isolated from the urine of patients with monocytic leukemia and has also been purified from adherent monocytes. The naturally-occurring, fully glycosylated form has an apparent molecular weight of about 25,000 Daltons. The protein shows 26% amino acid homology to IL-1 beta and 19% homology to IL-1 alpha . It will compete with either factor for receptor binding, but does not interact with either one. Human IL-1ra will bind to both types of IL-1 receptor (I and II) on human cells. In mouse, IL-1 RII does not bind IL-1ra. IL-1ra has been shown to block the inflammatory responses induced by IL-1 both in vitro and in vivo. Pre-clinical and clinical studies were done to test possible therapeutic applications for IL-1ra in the treatment of sepsis, rheumatoid arthritis and chronic myelogenous leukemia.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for IL-1ra/IL-1F3/IL1RN Antibody (BAF480)(4)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 1 application: ELISA Development.


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ELISA Development
(3)
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Secondary Antibodies

 

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Bioinformatics

Gene Symbol IL1RN