![Western Blot: Goat anti-Rat IgM Heavy Chain Secondary Antibody [DyLight 405] [NBP1-72982] - Western Blot of Goat anti-Rat IgM Heavy Chain Secondary antibody [DyLight 405]. Lane M: 3 ul Molecular Ladder. Lane 1: Rat IgG whole molecule. Lane 2: Rat IgG F(c) Fragment. Lane 3: Rat IgG Fab Fragment. Lane 4: Rat IgM Whole Molecule. Lane 5: Rat Serum. All samples were reduced. Load: 50 ng per lane. Block for 30 min at RT. Primary Antibody: Goat anti-Rat IgM Heavy Chain Secondary antibody [DyLight 405] 1:1,000 for 60 min at RT. Secondary Antibody: Anti-Goat IgG (DONKEY) Peroxidase Conjugated Antibody 1:40,000 in blocking buffer for 30 min at RT. Predicted/Obsevered Size: 25 and 55 kDa for Rat IgG and Serum, 25 kDa for F(c) and Fab, 78 and 25 kDa for IgM. Rat F(c) migrates slightly higher.](http://images.novusbio.com/fullsize/Goat-anti-Rat-IgM-Heavy-Chain-Secondary-Antibody-[DyLight-405]-Western-Blot-NBP1-72982-img0003.jpg "Western Blot: Goat anti-Rat IgM Heavy Chain Secondary Antibody [DyLight 405] [NBP1-72982] - Western Blot of Goat anti-Rat IgM Heavy Chain Secondary antibody [DyLight 405]. Lane M: 3 ul Molecular Ladder. Lane 1: Rat IgG whole molecule. Lane 2: Rat IgG F(c) Fragment. Lane 3: Rat IgG Fab Fragment. Lane 4: Rat IgM Whole Molecule. Lane 5: Rat Serum. All samples were reduced. Load: 50 ng per lane. Block for 30 min at RT. Primary Antibody: Goat anti-Rat IgM Heavy Chain Secondary antibody [DyLight 405] 1:1,000 for 60 min at RT. Secondary Antibody: Anti-Goat IgG (DONKEY) Peroxidase Conjugated Antibody 1:40,000 in blocking buffer for 30 min at RT. Predicted/Obsevered Size: 25 and 55 kDa for Rat IgG and Serum, 25 kDa for F(c) and Fab, 78 and 25 kDa for IgM. Rat F(c) migrates slightly higher.")
| Reactivity | RtSpecies Glossary |
| Applications | WB, ELISA, ICC/IF |
| Clonality | Polyclonal |
| Host | Goat |
| Conjugate | DyLight 405 |
| Concentration | LYOPH |
| Description | Store vial at 4C prior to restoration. For extended storage aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use. This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rat IgM coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Rat IgM and Rat Serum |
| Immunogen | Rat IgM whole molecule |
| Specificity | No reaction was observed against other rat heavy or light chain proteins. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Goat |
| Gene | IGHM |
| Purity | Multi-step |
| Dilutions |
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| Application Notes | This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms. The emission spectra for this DyLight(TM) conjugate match the principle output wavelengths of most common fluorescence instrumentation. |
| Storage | Store lyophilized antibody at 4C in the dark. Aliquot reconstituted liquid and store at -20C. Avoid freeze-thaw cycles. |
| Buffer | Lyophilized from 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free |
| Preservative | 0.01% Sodium Azide |
| Concentration | LYOPH |
| Purity | Multi-step |
| Reconstitution Instructions | Reconstitute with 100 ul deionized water (or equivalent). |
![Goat anti-Rat IgM Heavy Chain Secondary Antibody [DyLight 405]](/sites/all/modules/enterprise-tech/et_datasheets/images/novus_guarantee.png "Goat anti-Rat IgM Heavy Chain Secondary Antibody [DyLight 405]")
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| Gene Symbol | IGHM |
![Fluorophore-linked immunosorbent assay: Goat anti-Rat IgM Heavy Chain Secondary Antibody [DyLight 405] [NBP1-72982] - Goat anti-Rat IgM Heavy Chain Secondary antibody [DyLight 405]](http://images.novusbio.com/fullsize/Goat-anti-Rat-IgM-Heavy-Chain-Secondary-Antibody-[DyLight-405]-Fluorophore-linked-immunosorbent-assay-NBP1-72982-img0002.jpg "Fluorophore-linked immunosorbent assay: Goat anti-Rat IgM Heavy Chain Secondary Antibody [DyLight 405] [NBP1-72982] - Goat anti-Rat IgM Heavy Chain Secondary antibody [DyLight 405]")
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