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Goat anti-Mouse IgM Heavy Chain Secondary Antibody

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Immunocytochemistry/ Immunofluorescence: Goat anti-Mouse IgM Heavy Chain Secondary Antibody [NB120-9167] - Immunocytochemistry/ Immunofluorescence: Goat anti-Mouse IgM Secondary Antibody [DyLight 488] [NBP1-72873] Image ...read more
Fluorophore-linked immunosorbent assay: Goat anti-Mouse IgM Heavy Chain Secondary Antibody [NB120-9167] - Image using the DyLight 405 format of this antibody.
Fluorophore-linked immunosorbent assay: Goat anti-Mouse IgM Heavy Chain Secondary Antibody [NB120-9167] - Image using the DyLight 680 format of this antibody.
Fluorophore-linked immunosorbent assay: Goat anti-Mouse IgM Heavy Chain Secondary Antibody [NB120-9167] - Image using the DyLight 800 format of this antibody.
Fluorophore-linked immunosorbent assay: Goat anti-Mouse IgM Heavy Chain Secondary Antibody [NB120-9167] - Image using the DyLight 488 format of this antibody.
Dot Blot of Mouse IgM (mu chain) (GOAT) Antibody. Lane 1A: Mouse IgM (100 ng). Lane 1B: Mouse IgG (100 ng). Lanes 2-5: serial 3-fold dilution. Primary Antibody: Mouse IgM (mu chain) Antibody at 1 ug/mL at RT for 60 ...read more

Product Details

Summary
Reactivity MuSpecies Glossary
Applications WB, DB, ELISA, ICC/IF, IHC
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated

Goat anti-Mouse IgM Heavy Chain Secondary Antibody Summary

Description
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgM coupled to agarose followed by solid phase adsorption(s). Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Mouse IgM and Mouse Serum

Store vial at 4C prior to opening. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing.
Immunogen
Mouse IgM whole molecule
Specificity
No reaction was observed against other mouse heavy or light chain proteins.
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
IGHM
Purity
Multi-step

Applications/Dilutions

Dilutions
  • Dot Blot
  • ELISA 1:20000 - 1:50000
  • Fluorophore-linked immunosorbent assay
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry 1:1000 - 1:5000
  • Western Blot 1:500 - 1:2000
Application Notes
This product has been tested by ELISA, dot blot, and western blot and is suitable for immunoblotting (western or dot blot), ELISA, and immunohistochemistry assays requiring lot-to-lot consistency.
Publications
Read Publications using NB120-9167.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative
0.01% Sodium Azide
Purity
Multi-step

Alternate Names for Goat anti-Mouse IgM Heavy Chain Secondary Antibody

  • Constant region of heavy chain of IgM
  • Ig Mu Chain C Region BOT
  • Ig Mu Chain C Region GAL
  • Ig Mu Chain C Region OU
  • immunoglobulin heavy constant mu
  • Immunoglobulin mu chain

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for IgM Heavy Chain Antibody (NB120-9167)(2)

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Bioinformatics

Gene Symbol IGHM