Novus Biologicals products are now on bio-techne.com

Goat anti-Mouse IgG2 Secondary Antibody [Biotin]

Images

 
There are currently no images for IgG2 Antibody (NBP2-69549).

Every product we sell is backed by Novus' 100% Guarantee. If you have used this product, please submit your images and reviews to earn reward points.

Product Details

Summary
Reactivity MuSpecies Glossary
Applications WB, DB, ELISA, ICC/IF, IHC
Clonality
Polyclonal
Host
Goat
Conjugate
Biotin
Concentration
LYOPH

Order Details

View Available Conjugates
Novus Biologicals is part of Bio-Techne

Shop this product on bio-techne.com

Goat anti-Mouse IgG2 Secondary Antibody [Biotin] Summary

Description
The lyophilized conjugate is shipped at ambient temperature and may be stored at 4C; prolonged storage at or below -20C. Prior to use, an aliquot is thawed slowly at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at 4C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the immunoconjugate.
Immunogen
Pools of purified homogenous IgG2a and IgG2b isolated from mouse serum of strains belonging to the allelic types Igh-1a an Igh-1b. Freund's complete adjuvant is used in the first step of the immunization procedure.
Specificity
The reactivity of the antiserum is directed to the subclasses IgG2a and IgG2b. It does not react with other subclasses of IgG, IgG/Fab fragments, IgM and IgA or any non-Ig protein in mouse serum, as tested by immunoelectrophoresis and double radial immunodiffusion.
Clonality
Polyclonal
Host
Goat
Gene
IGHG2
Purity
Ion exchange chromatography

Applications/Dilutions

Dilutions
  • Dot Blot
  • ELISA 1:100-1:2000
  • Immunocytochemistry/ Immunofluorescence 1:10 - 1:500
  • Immunohistochemistry-Paraffin
  • Western Blot
Application Notes
.

Reactivity Notes

This IgG fraction is not species-specific since inter-species cross-reactivity is a normal feature of antisera to immunoglobulins. However this product has been passed over appropriate immunoadsorbents to remove antibodies cross-reacting with Human immunoglobulins. This renders it specific for use in test systems containing material of Human origin (e.g. Human tissue/Mouse monoclonal antibody to a Human tissue constituent/anti Mouse Ig isotype-specific immunoconjugate. If the IgG fraction is to be used in the presence of material originating from another species, prior screening for cross-reactivity is essential an additional adsorption of the reagent may required to ensure intra-assay specificity. In double radial immunodiffusion a reaction with Rat has been observed.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
Lyophilized from PBS (pH 7.2)
Preservative
No Preservative
Concentration
LYOPH
Purity
Ion exchange chromatography
Reconstitution Instructions
Reconstitute by by adding 1 ml sterile distilled water, spun down to remove insoluble particles, divided into small aliquots, frozen and stored at or below -20C.

Alternate Names for Goat anti-Mouse IgG2 Secondary Antibody [Biotin]

  • Constant region of heavy chain of IgG2
  • immunoglobulin gamma 2 (Gm marker)
  • immunoglobulin heavy constant gamma 2 (Gm marker)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

NBP2-79716
Species: Hu
Applications: IHC, IHC-P, PA
NBP3-11412
Species: Sh
Applications: ELISA, IHC, IHC-Fr, WB
NBP2-61794
Species: Hu, Pm, Mu
Applications: ELISA, Flow, ICC/IF, IHC, IHC-P, WB
NB7276
Species: Ch
Applications: ELISA, ICC/IF, IHC, IHC-P, WB
291-G1
Species: Hu
Applications: BA
NB100-62667
Species: Hu
Applications: CyTOF-ready, ELISA, Flow, IHC, IHC-Fr, IHC-P, IP
NBP2-62025
Species: Hu
Applications: ELISA, ICC/IF, IHC, IHC-P, ELISA(Cap), ELISA(Det)
H00003216-M01-100ug
Species: Hu
Applications: ELISA, WB
H00003217-M03
Species: Hu, Rb
Applications: ELISA, Func, IHC, IHC-P, WB
NBP2-83058
Species: Hu
Applications: WB
AF1938
Species: Hu
Applications: ICC, IP, Simple Western, WB
H00003219-M05
Species: Hu
Applications: ChIP, ELISA, IP, S-ELISA, WB
NBP2-02043
Species: Hu, Rt
Applications: Flow, ICC/IF, IHC, IHC-P, WB
NBP1-87492
Species: Hu
Applications: IHC, IHC-P, WB

Publications for IgG2 Antibody (NBP2-69549) (0)

There are no publications for IgG2 Antibody (NBP2-69549).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for IgG2 Antibody (NBP2-69549) (0)

There are no reviews for IgG2 Antibody (NBP2-69549). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for IgG2 Antibody (NBP2-69549) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Additional IgG2 Products

Research Areas for IgG2 Antibody (NBP2-69549)

Find related products by research area.

Blogs on IgG2

There are no specific blogs for IgG2, but you can read our latest blog posts.
mFluor Violet Conjugated Antibodies

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our Goat anti-Mouse IgG2 Secondary Antibody [Biotin] and receive a gift card or discount.

Bioinformatics

Gene Symbol IGHG2