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IgG Antibody (97924) [Unconjugated] - Fc region

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(A) Recombinant Human B7-H1/PD-L1 Fc Chimera (Catalog # 156-B7) bound to HEK293 human embryonic kidney cell line transfected with human PD-1 and eGFP was stained with Mouse Anti-Human IgG Fc Monoclonal Antibody (Catalog ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Flow, CyTOF-ready
Clone
97924
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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IgG Antibody (97924) [Unconjugated] - Fc region Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant human IgG1 Fc region
Specificity
Detects chimeric proteins containing a human IgG Fc region in direct ELISAs and Western blots.
Source
N/A
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
IGHG1
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • CyTOF-ready
  • Flow Cytometry 0.25 ug/10^6 cells
  • Western Blot 1 ug/mL
Reviewed Applications
Read 1 Review rated 4
using
MAB110 in the following applications:

Publications
Read Publications using
MAB110 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS and NaCl with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for IgG Antibody (97924) [Unconjugated] - Fc region

  • IgG
  • Immunoglobulin G
  • ImmunoglobulinG

Background

R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels.

Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for IgG Antibody (MAB110)(11)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 5 applications: Control, ELISA Development, Neutralization, Neutralization Control, Western Blot.


Filter By Application
Control
(1)
ELISA Development
(2)
Neutralization
(2)
Neutralization Control
(1)
Western Blot
(1)
All Applications
Filter By Species
Human
(5)
Mouse
(2)
All Species
Showing Publications 1 - 10 of 11. Show All 11 Publications.
Publications using MAB110 Applications Species
Kummarapurugu, AB;Hawkridge, AM;Ma, J;Osei, S;Martin, RK;Zheng, S;Voynow, JA; Neutrophil Elastase decreases SARS-CoV-2 Spike protein binding to human bronchial epithelia by clipping ACE-2 ectodomian from the epithelial surface The Journal of biological chemistry 2023-05-13 [PMID: 37187291] (Western Blot, Human) Western Blot Human
Ivette Mendoza-Salazar, Keyla M. Gómez-Castellano, Edith González-González, Ramsés Gamboa-Suasnavart, Stefany D. Rodríguez-Luna, Giovanni Santiago-Casas et al. Anti-SARS-CoV-2 Omicron Antibodies Isolated from a SARS-CoV-2 Delta Semi-Immune Phage Display Library Antibodies (Basel) 2/10/2022 [PMID: 35225871]
Caitlyn L. Miller, Idit Sagiv-Barfi, Patrick Neuhöfer, Debra K. Czerwinski, Steven E. Artandi, Carolyn R. Bertozzi et al. Systemic delivery of a targeted synthetic immunostimulant transforms the immune landscape for effective tumor regression Cell Chemical Biology 3/1/2022 [PMID: 34774126]
M Yang, G Zhang, Y Wang, M He, Q Xu, J Lu, H Liu, C Xu Tumour-associated neutrophils orchestrate intratumoural IL-8-driven immune evasion through Jagged2 activation in ovarian cancer Br. J. Cancer, 2020-08-11;0(0):. 2020-08-11 [PMID: 32778818] (ELISA Development, Human) ELISA Development Human
Y Yoon, G Voloudakis, N Doran, E Zhang, C Dimovasili, L Chen, Z Shao, S Darmanis, C Tang, J Tang, VX Wang, PR Hof, NK Robakis, A Georgakopo PS1 FAD mutants decrease ephrinB2-regulated angiogenic functions, ischemia-induced brain neovascularization and neuronal survival Mol. Psychiatry, 2020-06-15;0(0):. 2020-06-15 [PMID: 32541930] (Neutralization, Mouse) Neutralization Mouse
Chen Q, Liu J, Sawada T et al. Possible role of EphA4 and VEGFR2 interactions in neural stem and progenitor cell differentiation Exp Ther Med 1/3/2020 [PMID: 32104234]
Li Zhong, Zongqi Wang, Daxin Wang, Zhe Wang, Yuka A. Martens, Linbei Wu et al. Amyloid-beta modulates microglial responses by binding to the triggering receptor expressed on myeloid cells 2 (TREM2) Molecular Neurodegeneration 12/1/2018 [PMID: 29587871]
Activated monocytes resist elimination by retinal pigment epithelium and downregulate their OTX2 expression via TNF-? Aging Cell, 2016-09-22;0(0):. 2016-09-22 [PMID: 27660103] (Neutralization, Human) Neutralization Human
Wan-Chien Cheng Periodontitis-associated pathogens P. gingivalis and A. actinomycetemcomitans activate human CD14(+) monocytes leading to enhanced Th17/IL-17 responses Eur J Immunol, 2016-08-01;0(0):. 2016-08-01 [PMID: 27334899] (Control, Human) Control Human
Kotani Y, Kunisawa J, Suzuki Y, Sato I, Saito T, Toba M, Kohda N, Kiyono H Role of Lactobacillus pentosus Strain b240 and the Toll-like receptor 2 axis in Peyer's patch dendritic cell-mediated immunoglobulin A enhancement. PLoS ONE, 2014-03-14;9(3):e91857. 2014-03-14 [PMID: 24632732] (Neutralization Control, Mouse) Neutralization Control Mouse
Show All 11 Publications.

Review for IgG Antibody (MAB110) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using MAB110:
Filter by Applications
WB
(1)
All Applications
Filter by Species
Human
(1)
All Species
Images Ratings Applications Species Date Details
  4
reviewed by:
Verified Customer
WB Human 02/04/2015
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Summary

ApplicationWestern Blot
Sample TestedSee PMID 22820181
SpeciesHuman

Product General Protocols

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FAQs for IgG Antibody (MAB110) (0)

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Secondary Antibodies

 

Isotype Controls

Additional IgG Products

Blogs on IgG.

Early T cell response is associated with mild COVID-19 and rapid SARS-CoV-2 clearance
Jamshed Arslan, Pharm D, PhD SARS-CoV-2 induces both humoral and cellular immunity. A vaccine or natural infection invokes SARS-CoV-2-specific humoral components (antibodies from activated B cells) and cellular resp...  Read full blog post.


  Read full blog post.

CD16 - Find me on macrophages, neutrophils and NK cells
CD16 is a lymphocyte Fc gamma type III low-affinity receptor for IgG and is represented by two similar genes, CD16A (Fc gamma RIII A) and CD16B (Fc gamma RIIIB). CD16A exists as a heterooligomeric polypeptide-anchored form in macrophages and NK cells....  Read full blog post.

Understanding the Importance of Human IgG
Human IgG is a component of the immune system that protects the body from infection. It is the most abundantly found antibody isotype within the circulatory system of the human body. All antibody isotypes contain two heavy chains and two light chains ...  Read full blog post.

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Recent Reviews

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Verified Customer
02/04/2015
Application: WB
Species: Human

Bioinformatics

Gene Symbol IGHG1