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IGF-II R/IGF2R Antibody (2G11) - Azide and BSA Free

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Immunocytochemistry/Immunofluorescence: IGF-II R/IGF2R Antibody (2G11) - Azide and BSA Free [NBP2-80785] - Staining in Hela Cells. Mannose 6-Phosphate Receptor staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with an antibody recognizing Mannose 6-Phosphate Receptor at a dilution of 1:20 over night at 4 C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody. Image from the standard format of this antibody.
Immunocytochemistry/ Immunofluorescence: IGF-II R/IGF2R Antibody (2G11) - Azide and BSA Free [NBP2-80785] - Staining in Hela Cells. Mannose 6-Phosphate Receptor staining (green), F-Actin staining with Phalloidin (red) ...read more
Immunohistochemistry: IGF-II R/IGF2R Antibody (2G11) - Azide and BSA Free [NBP2-80785] - Staining of human testis using NB300-514 antibody at 1:100. Image from the standard format of this antibody.
Flow Cytometry: IGF-II R/IGF2R Antibody (2G11) - Azide and BSA Free [NBP2-80785] - An intracellular stain was performed on MCF7 cells with IGF-II R/IGF2R [2G11] Antibody NB300-514AF647 (blue) and a matched isotype ...read more
Immunocytochemistry/ Immunofluorescence: IGF-II R/IGF2R Antibody (2G11) - Azide and BSA Free [NBP2-80785] - HMVEC Cells Image from the standard format of this antibody.
Immunocytochemistry/ Immunofluorescence: IGF-II R/IGF2R Antibody (2G11) - Azide and BSA Free [NBP2-80785] - Mannose 6 Phosphate Receptor (Cation independent) antibody (2G11) was tested at (1:250) in HeLa cells with ...read more
Immunocytochemistry/ Immunofluorescence: IGF-II R/IGF2R Antibody (2G11) - Azide and BSA Free [NBP2-80785] - Mannose 6-Phosphate Receptor staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI ...read more
Flow Cytometry: IGF-II R/IGF2R Antibody (2G11) - Azide and BSA Free [NBP2-80785] - Intracellular flow cytometric staining of 1 x 10^6 MCF-7 cells using Mannose 6 Phosphate Receptor antibody (dark blue). Isotype control ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, Bv, PmSpecies Glossary
Applications WB, ELISA, Flow, ICC/IF, IHC, IP, CyTOF-ready
Clone
2G11
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
Azide and BSA Free
Concentration
1 mg/ml

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NB300-514AF350
Alexa Fluor 405
NB300-514AF405
Alexa Fluor 488
NB300-514AF488
Alexa Fluor 532
NB300-514AF532
Alexa Fluor 594
NB300-514AF594
Alexa Fluor 647
NB300-514AF647
Alexa Fluor 700
NB300-514AF700
Alexa Fluor 750
NB300-514AF750
Allophycocyanin
NB300-514APC
Biotin
NB300-514B
CoraFluor 1
NB300-514CL1
DyLight 350
NB300-514UV
DyLight 405
NB300-514V
DyLight 488
NB300-514G
DyLight 550
NB300-514R
DyLight 594
NB300-514DL594
DyLight 650
NB300-514C
DyLight 680
NB300-514FR
DyLight 755
NB300-514IR
FITC
NB300-514F
HRP
NB300-514H
Janelia Fluor 525
NB300-514JF525
Janelia Fluor 549
NB300-514JF549
Janelia Fluor 646
NB300-514JF646
Janelia Fluor 669
NB300-514JF669
PE
NB300-514PE
Unconjugated
NB300-514
mFluor Violet 450 SE
NB300-514MFV450
mFluor Violet 500 SE
NB300-514MFV500
mFluor Violet 610 SE
NB300-514MFV610
View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
NB300-514
BSA Free
NB300-514SS
BSA Free
NB300-514
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IGF-II R/IGF2R Antibody (2G11) - Azide and BSA Free Summary

Immunogen
Purified bovine Mannose 6 Phosphate Receptor (Cation independent) [UniProt# P08169]
Epitope
In the extracellular domain of Mannose 6 Phosphate Receptor (Cation independent).
Isotype
IgG2a Kappa
Clonality
Monoclonal
Host
Mouse
Gene
IGF2R
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • CyTOF-ready
  • ELISA
  • Flow Cytometry 1 ug per million cells
  • Immunocytochemistry/ Immunofluorescence 1:10-1:250
  • Immunohistochemistry 1:100
  • Immunohistochemistry-Paraffin 1:100
  • Immunoprecipitation 1:10-1:500
  • Western Blot 1 ug/ml
Application Notes
By Western Blot, a 300 kDa protein representing Mannose 6 Phosphate Receptor is seen in HeLa cell extract under non-reducing conditions. Immunofluorescence staining of Mannose 6 Phosphate Receptor in HeLa cells with this antibody results in perinuclear staining. This antibody is also useful in Flow Cytometry, Immunoprecipitation, ELISA and Immunohistochemistry-on paraffin embedded sections. This antibody is CyTOF ready.

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 9119492). Does not react with Chinese hamster ovary cell Mannose 6 Phosphate Receptor.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
No Preservative
Concentration
1 mg/ml
Purity
Protein A purified

Alternate Names for IGF-II R/IGF2R Antibody (2G11) - Azide and BSA Free

  • 300 kDa mannose 6-phosphate receptor
  • cation-independent mannose-6 phosphate receptor
  • cation-independent mannose-6-phosphate receptor
  • CD222 antigen
  • CD222
  • CI Man-6-P receptor
  • CIMPR
  • CI-MPR
  • IGF2R
  • IGF-II R
  • IGF-II receptor
  • IGFIIR
  • IGF-IIR
  • insulin-like growth factor 2 receptorM6P/IGF2R
  • Insulin-like growth factor II receptor
  • M6P/IGF2 receptor
  • M6PR
  • M6P-R
  • MPR 300
  • MPR1
  • MPRI
  • MPRIM6PR

Background

Mannose 6 phosphate is a sequence tag that plays a pivotal role in delivering proteins to the lysosome. Specifically, the mannose 6 phosphate sequence is tagged to N-linked oligosaccharides of lysosomal hydrolases as they pass through the cis-Golgi network. Once the lysosomal enzymes are tagged with the M6P sequence, they are destined to the late endosome by way of vesicular transport. After a low pH dissociates the M6P Receptor from its ligand, the M6P sequences are recycled. Additionally, M6P is bound by lectin in the immune system, and is converted to fructose 6-phosphate by mannose phosphate isomerase.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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IGF-II R/IGF2R
Antibody (2G11) - Azi ...
NBP2-80785
IGF-II R/IGF2R Antibody (2G11) - Azide and BSA Free
Species: Hu, Mu, Rt, Bv, Pm
Applications: WB, ELISA, Flow, ICC/IF, IHC, IP, CyTOF-ready

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Bioinformatics

Gene Symbol IGF2R