Reactivity | HuSpecies Glossary |
Applications | Bioactivity |
Format | Carrier-Free |
Concentration | LYOPH |
Description | A single non-glycosylated polypeptide chain containing 67 amino acids corresponding to IGF-II/IGF2 Source: E. coli Uniprot ID: E3UN46 Amino Acid Sequence: AYRPSETLCG GELVDTLQFV CGDRGFYFSR PASRVSRRSR GIVEECCFRS CDLALLETYC ATPAKSE |
Details of Functionality | IGF-II/IGF2 protein is fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using serum free human MCF-7 cells is less than 2 ng/ml, corresponding to a specific activity of > 5.0 x 10^5 IU/mg. |
Source | E. coli |
Protein/Peptide Type | Recombinant Protein |
Gene | IGF2 |
Purity | > 98 % pure by SDS-PAGE and HPLC |
Endotoxin Note | Less than 1 EU/ug of IGF-II/IGF2 as determined by LAL method. |
Dilutions |
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Theoretical MW | 7.5 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
Storage | Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles. |
Buffer | Lyophilized from a 0.2 um filtered concentrated solution in 20 mM Tris-HCl, pH 8.0, 150 mM NaCl, with 0.02 % Tween-20. |
Preservative | No Preservative |
Concentration | LYOPH |
Purity | > 98 % pure by SDS-PAGE and HPLC |
Reconstitution Instructions | Recommended to centrifuge prior to opening. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/mL. Apportion stock solutions into working aliquots and store at <-20C. |
Research Areas for IGF-II/IGF2 Recombinant Protein (NBP2-59722)Find related products by research area.
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Gene Symbol | IGF2 |