![Western Blot: IGF-II Antibody (8H1) [NBP2-37462] - Analysis using IGF2 mAb against HEK293 (1) and IGF2 (AA: 25-180)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/IGF-II-Antibody-8H1-Western-Blot-NBP2-37462-img0003.jpg "Western Blot: IGF-II Antibody (8H1) [NBP2-37462] - Analysis using IGF2 mAb against HEK293 (1) and IGF2 (AA: 25-180)-hIgGFc transfected HEK293 (2) cell lysate.")
| Reactivity | HuSpecies Glossary |
| Applications | WB, ELISA, Flow, ICC/IF, IHC |
| Clone | 8H1 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Format | BSA Free |
| Concentration | 1 mg/ml |
| Immunogen | Purified recombinant fragment of human IGF-II/IGF2 (AA: 25-180) expressed in E. Coli. |
| Isotype | IgG1 |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | IGF2 |
| Purity | Protein G purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Theoretical MW | 20.1 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
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| Publications |
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| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS |
| Preservative | 0.05% Sodium Azide |
| Concentration | 1 mg/ml |
| Purity | Protein G purified |
![Western Blot IGF-I R/IGF1R Antibody (33255) [Unconjugated]](https://images.novusbio.com/images2/IGF-I_R_MAB391_Western_Blot_6136.jpg)
![Immunohistochemistry IGF-I R/IGF1R Antibody (33255) [Unconjugated]](https://images.novusbio.com/images2/IGFI_R_MAB391_Immunohistochemistry_21743.jpg)
![Flow Cytometry IGF-I R/IGF1R Antibody (33255) [Unconjugated]](https://images.novusbio.com/images2/IGFI_R_MAB391_Flow_Cytometry_22536.jpg)
| Publication using NBP2-37462 | Applications | Species |
|---|---|---|
| Yamasato Kelly, Tsai Pai-Jong Stacy, Davis James et al. Human relaxins (RLNH1, RLNH2), their receptor (RXFP1) and fetoplacental growth. Reproduction (Cambridge, England) 2017-01-01 [PMID: 28468839] (IF/IHC, Human) | IF/IHC | Human |
Secondary Antibodies |
Isotype Controls |
Research Areas for IGF-II/IGF2 Antibody (NBP2-37462)Find related products by research area.
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The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
| Gene Symbol | IGF2 |
![Immunocytochemistry/Immunofluorescence: IGF-II/IGF2 Antibody (8H1) [NBP2-37462] - Analysis of HeLa cells using IGF2 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.](http://images.novusbio.com/fullsize/IGF-II-Antibody-8H1-Immunocytochemistry-Immunofluorescence-NBP2-37462-img0004.jpg "Immunocytochemistry/Immunofluorescence: IGF-II/IGF2 Antibody (8H1) [NBP2-37462] - Analysis of HeLa cells using IGF2 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.")
![Immunohistochemistry-Paraffin: IGF-II/IGF2 Antibody (8H1) [NBP2-37462] - Analysis of paraffin-embedded bladder cancer tissues using IGF-II/IGF2 (8H1)mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/IGF-II-IGF2-Antibody-8H1-Immunohistochemistry-Paraffin-NBP2-37462-img0007.jpg "Immunohistochemistry-Paraffin: IGF-II/IGF2 Antibody (8H1) [NBP2-37462] - Analysis of paraffin-embedded bladder cancer tissues using IGF-II/IGF2 (8H1)mouse mAb with DAB staining.")
![Flow Cytometry: IGF-II/IGF2 Antibody (8H1) [NBP2-37462] - Analysis of HepG2 cells using IGF2 mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/IGF-II-Antibody-8H1-Flow-Cytometry-NBP2-37462-img0005.jpg "Flow Cytometry: IGF-II/IGF2 Antibody (8H1) [NBP2-37462] - Analysis of HepG2 cells using IGF2 mouse mAb (green) and negative control (red).")
![Western Blot: IGF-II/IGF2 Antibody (8H1) [NBP2-37462] - Analysis using IGF2 mAb against human IGF2 recombinant protein. (Expected MW is 43.1 kDa)](http://images.novusbio.com/fullsize/IGF-II-Antibody-8H1-Western-Blot-NBP2-37462-img0002.jpg "Western Blot: IGF-II/IGF2 Antibody (8H1) [NBP2-37462] - Analysis using IGF2 mAb against human IGF2 recombinant protein. (Expected MW is 43.1 kDa)")
![Immunohistochemistry-Paraffin: IGF-II/IGF2 Antibody (8H1) [NBP2-37462] - Analysis of ovarian cancer tissues using IGF2 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/IGF-II-Antibody-8H1-Immunohistochemistry-NBP2-37462-img0006.jpg "Immunohistochemistry-Paraffin: IGF-II/IGF2 Antibody (8H1) [NBP2-37462] - Analysis of ovarian cancer tissues using IGF2 mouse mAb with DAB staining.")
![ELISA: IGF-II/IGF2 Antibody (8H1) [NBP2-37462] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);](http://images.novusbio.com/fullsize/IGF-II-Antibody-8H1-ELISA-NBP2-37462-img0001.jpg "ELISA: IGF-II/IGF2 Antibody (8H1) [NBP2-37462] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);")
![SDS-PAGE IGF-I/IGF-1 [Unconjugated]](https://images.novusbio.com/images2/IGF-I_291-G1_40.jpg)
![Bioactivity IGF-I/IGF-1 [Unconjugated]](https://images.novusbio.com/images2/IGF-I_291-G1_41.jpg)
![Mass Spectrometry IGF-I/IGF-1 [Unconjugated]](https://images.novusbio.com/images2/IGF-I_291-G1_42.jpg)
![N/A IGFBP-3 [HRP]](https://images.novusbio.com/images2/DATA_IGFBP3_DGB300_ELISA_694.jpg)
![N/A IGFBP-3 [HRP]](https://images.novusbio.com/images2/IGFBP-3_DGB300_ELISA_104.jpg)
![Immunocytochemistry Insulin Antibody (182410) [Unconjugated]](https://images.novusbio.com/images2/Insulin_MAB1417_Immunocytochemistry_9376.jpg)
![Immunohistochemistry Insulin Antibody (182410) [Unconjugated]](https://images.novusbio.com/images2/mab1417_human-bovine-mouse-insulin-mab-clone-182410-immunohistochemistry-308202115145.jpg)
![N/A IGFBP-1 [Biotin]](https://images.novusbio.com/images2/DATA_IGFBP1_DY871_ELISA_2250.jpg)
![Western Blot IGFBP-2 Antibody [Unconjugated]](https://images.novusbio.com/images2/IGFBP2_AF674_Western_Blot_20670.jpg)
![Simple Western IGFBP-2 Antibody [Unconjugated]](https://images.novusbio.com/images2/IGFBP2_AF674_Simple_Western_20942.jpg)
![Neutralization IGFBP-2 Antibody [Unconjugated]](https://images.novusbio.com/images2/IGFBP-2_AF674_Block_Neutralize_8943.jpg)
![Western Blot Growth Hormone Antibody [Unconjugated]](https://images.novusbio.com/images2/Growth_Hormone_AF1067_Western_Blot_20961.jpg)
![ELISA Growth Hormone Antibody [Unconjugated]](https://images.novusbio.com/images2/Growth_Hormone_AF1067_ELISA_22201.jpg)
![Neutralization Growth Hormone Antibody [Unconjugated]](https://images.novusbio.com/images2/Growth_Hormone_AF1067_Block_Neutralize_8751.jpg)
![SDS-PAGE EGF [Unconjugated]](https://images.novusbio.com/images2/EGF_236-EG_234.jpg)
![Bioactivity EGF [Unconjugated]](https://images.novusbio.com/images2/EGF_236EG_1570.jpg)
![Cell Culture EGF [Unconjugated]](https://images.novusbio.com/images/236-eg_recombinant-human-egf-protein-cf-bioactivity-25202394053.jpg)
![SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.](https://images.novusbio.com/images/Mouse-IgG1-Isotype-Control-MG1-SDS-Page-NBP1-97005-img0002.jpg "SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.")