Home » IGF-I/IGF-1 » IGF-I/IGF-1 Antibodies » IGF-I/IGF-1 Antibody (56408) [Unconjugated]

IGF-I/IGF-1 Antibody (56408) [Unconjugated]

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Western blot shows 25 ng of Recombinant Human IGF-I/IGF-1 (Catalog # 291-G1), Recombinant Mouse IGF-I/IGF-1 (Catalog # 791-MG), Recombinant Rat IGF-I/IGF-1 (Catalog # 4326-RG), and Recombinant Human IGF-II (Catalog # 292-G2). PVDF Membrane was probed with 1 µg/mL of Mouse Anti-Human IGF-I/IGF-1 Monoclonal Antibody (Catalog # MAB291) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for IGF-I/IGF-1 at approximately 10 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3.
Western blot shows 25 ng of Recombinant Human IGF-I/IGF-1 (Catalog # 291-G1), Recombinant Mouse IGF-I/IGF-1 (Catalog # 791-MG), Recombinant Rat IGF-I/IGF-1 (Catalog # 4326-RG), and Recombinant Human IGF-II (Catalog # ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA(Cap)
Clone
56408
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
MAB291-SP
Carrier Free
MAB291-500
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IGF-I/IGF-1 Antibody (56408) [Unconjugated] Summary

Immunogen
E. coli-derived recombinant human IGF-I/IGF-1
Gly49-Ala118
Accession # P01343
Specificity
Detects human IGF-I/IGF-1 in ELISAs and Western blots. In ELISAs, this antibody does not cross-react with recombinant mouse (rm) IGF-I/IGF-1, rmIGF‑II/IGF-2, or recombinant human IGF‑II/IGF-2.
Source
N/A
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
IGF1
Purity Statement
Protein A or G purified from ascites
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
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Applications/Dilutions

Dilutions
  • ELISA Capture (Matched Antibody Pair) 2-8 ug/mL
  • ELISA Detection (Matched Antibody Pair) 0.1-0.4 ug/mL
  • ELISA Standard (Matched Pair)
  • Western Blot 1 ug/mL
Application Notes
ELISA Detection: Human IGF-I Biotinylated Antibody (Catalog number BAF291)
Standard: Recombinant Human IGF-I (Catalog number 291-G1)
Publications
Read Publications using
MAB291 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for IGF-I/IGF-1 Antibody (56408) [Unconjugated]

  • IBP1
  • IGF1
  • IGF-1
  • IGF1A
  • IGFI
  • IGF-I
  • IGF-IA
  • IGF-IB
  • insulin-like growth factor 1 (somatomedin C)
  • insulin-like growth factor 1
  • insulin-like growth factor I
  • insulin-like growth factor IA
  • insulin-like growth factor IB
  • Mechano growth factor
  • MGF
  • Somatomedin A
  • Somatomedin C
  • somatomedin-C

Background

IGF-I/IGF-1 belongs to the family of insulin-like growth factors and circulates in complex with IGF binding proteins. It is a potent mitogenic growth factor that binds the heteromeric type I and type II IGF receptors. Essentially, all of the biological activities of IGF-I/IGF-1 are mediated by IGF-I R.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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IGF-I/IGF-1
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MAB291
IGF-I/IGF-1 Antibody (56408) [Unconjugated]
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Publications for IGF-I/IGF-1 Antibody (MAB291)(7)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 4 applications: ELISA Development, ELISA Development (Capture), ELISA Microarray Development, Western Blot.


Filter By Application
ELISA Development
(1)
ELISA Development (Capture)
(1)
ELISA Microarray Development
(1)
Western Blot
(2)
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Human
(5)
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Showing Publications 1 - 7 of 7.
Publications using MAB291 Applications Species
Jaeman Lee, Kyeong-Ryang Ko, Nayeon Lee, Sojin Kim, Seung-Shin Yu, Sunyoung Kim et al. Construction of Plasmid DNA Expressing Two Isoforms of Insulin-Like Growth Factor-1 and Its Effects on Skeletal Muscle Injury Models Human Gene Therapy 12/1/2022 [PMID: 35838121]
2020-12-17;0(0):. 2020-12-17 [PMID: 33348564] (Western Blot, Human) Western Blot Human
Landowski C, Huuskonen A, Wahl R, Westerholm-Parvinen A, Kanerva A, Hanninen A, Salovuori N, Penttila M, Natunen J, Ostermeier C, Helk B, Saarinen J, Saloheimo M Enabling Low Cost Biopharmaceuticals: A Systematic Approach to Delete Proteases from a Well-Known Protein Production Host Trichoderma reesei. PLoS ONE, 2015-08-26;10(8):e0134723. 2015-08-26 [PMID: 26309247] (Western Blot, Human) Western Blot Human
Luo X, Suzuki M, Ghandhi S, Amundson S, Boothman D ATM regulates insulin-like growth factor 1-secretory clusterin (IGF-1-sCLU) expression that protects cells against senescence. PLoS ONE, 2014-06-17;9(6):e99983. 2014-06-17 [PMID: 24937130] (ELISA Development (Capture), Human) ELISA Development (Capture) Human
E M Goetz, B Shankar, Y Zou, J C Morales, X Luo, S Araki et al. ATM-dependent IGF-1 induction regulates secretory clusterin expression after DNA damage and in genetic instability Oncogene 9/1/2011 [PMID: 21460853]
Gonzalez RM, Seurynck-Servoss SL, Crowley SA Development and validation of sandwich ELISA microarrays with minimal assay interference. J. Proteome Res., 2008-04-19;7(6):2406-14. 2008-04-19 [PMID: 18422355] (ELISA Microarray Development, Human) ELISA Microarray Development Human
Yin D, Vreeland F, Schaaf LJ, Millham R, Duncan BA, Sharma A Clinical pharmacodynamic effects of the growth hormone receptor antagonist pegvisomant: implications for cancer therapy. Clin. Cancer Res., 2007-02-01;13(3):1000-9. 2007-02-01 [PMID: 17289896] (ELISA Development, Human) ELISA Development Human

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Bioinformatics

Gene Symbol IGF1
Uniprot