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IGF-I/IGF-1 Antibody (126002) [Unconjugated]

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Recombinant Mouse IGF-I/IGF-1 (Catalog # 791-MG) stimulates proliferation in the MCF‑7 human breast cancer cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Mouse IGF-I/IGF-1 (15 ng/mL) is neutralized (green line) by increasing concentrations of Mouse IGF-I/IGF-1 Monoclonal Antibody (Catalog # MAB791). The ND50 is typically 0.3-1.5 µg/mL.
Recombinant Mouse IGF-I/IGF-1 (Catalog # 791-MG) stimulates proliferation in the MCF‑7 human breast cancer cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Mouse IGF-I/IGF-1 ...read more

Product Details

Summary
Reactivity MuSpecies Glossary
Applications WB, B/N, ELISA(Cap)
Clone
126002
Clonality
Monoclonal
Host
Hamster
Conjugate
Unconjugated

Order Details

View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
MAB791-SP
Carrier Free
MAB791-500
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IGF-I/IGF-1 Antibody (126002) [Unconjugated] Summary

Immunogen
E. coli-derived recombinant mouse IGF-I/IGF-1
Gly49-Ala118
Accession # P05017
Specificity
Detects mouse IGF-I/IGF-1 in direct ELISAs and Western blots. In ELISAs, no cross-reactivity with recombinant human IGF-I/IGF-1, recombinant mouse (rm) IGF-II/IGF-2, rmIGFBP-2, rmIGFBP-5, or rmIGFBP-6 is observed.
Source
N/A
Isotype
IgG
Clonality
Monoclonal
Host
Hamster
Gene
IGF1
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Endotoxin Note
<0.10 EU per 1 μg of the antibody by the LAL method.
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • ELISA Capture (Matched Antibody Pair) 2-8 ug/mL
  • ELISA Detection (Matched Antibody Pair) 0.1-0.4 ug/mL
  • ELISA Standard (Matched Pair)
  • Neutralization 0.3-1.5 ug/mL
  • Western Blot 1 ug/mL
Application Notes
ELISA Detection: Mouse IGF-I Biotinylated Antibody (Catalog number BAF791)
Standard: Recombinant Mouse IGF-I (Catalog number 791-MG)
Publications
Read Publications using
MAB791 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for IGF-I/IGF-1 Antibody (126002) [Unconjugated]

  • IBP1
  • IGF1
  • IGF-1
  • IGF1A
  • IGFI
  • IGF-I
  • IGF-IA
  • IGF-IB
  • insulin-like growth factor 1 (somatomedin C)
  • insulin-like growth factor 1
  • insulin-like growth factor I
  • insulin-like growth factor IA
  • insulin-like growth factor IB
  • Mechano growth factor
  • MGF
  • Somatomedin A
  • Somatomedin C
  • somatomedin-C

Background

Insulin-like growth factor I, also known as somatomedin C, is the dominant effector of growth hormone and is structurally homologous to proinsulin. Mouse IGF-I/IGF-1 is synthesized as two precursor isoforms with alternate N- and C-terminal propeptides (1). These isoforms are differentially expressed by various tissues (1). The 7.6 kDa mature IGF-I/IGF-1 is identical between isoforms and is generated by proteolytic removal of the N- and C-terminal regions. Mature mouse IGF-I/IGF-1 shares 94% and 99% amino acid (aa) sequence identity with human and rat IGF-I/IGF-1, respectively (2), and exhibits cross-species activity. It shares 60% aa sequence identity with mature mouse IGF-II/IGF-2. Circulating IGF-I/IGF-1 is produced by hepatocytes, while local IGF-I/IGF-1 is produced by many other tissues in which it has paracrine effects (1). IGF-I/IGF-1 induces the proliferation, migration, and differentiation of a wide variety of cell types during development and postnatally (3). IGF-I/IGF-1 regulates glucose and fatty acid metabolism, steroid hormone activity, and cartilage and bone metabolism (4-7). It plays an important role in muscle regeneration and tumor progression (1, 8). IGF-I/IGF-1 binds IGF-I R, IGF-II R, and the insulin receptor, although its effects are mediated primarily by IGF-I R (9). IGF-I/IGF-1 association with IGF binding proteins increases its plasma half-life and modulates its interactions with receptors (10).

  1. Philippou, A. et al. (2007) In Vivo 21:45.
  2. Bell, G.I. et al. (1986) Nucleic Acids Res. 14:7873.
  3. Guvakova, M.A. (2007) Int. J. Biochem. Cell Biol. 39:890.
  4. Clemmons, D.R. (2006) Curr. Opin. Pharmacol. 6:620.
  5. Bluher, S. et al. (2005) Best Pract. Res. Clin. Endocrinol. Metab. 19:577.
  6. Garcia-Segura, L.M. et al. (2006) Neuroendocrinology 84:275.
  7. Malemud, C.J. (2007) Clin. Chim. Acta 375:10.
  8. Samani, A.A. et al. (2007) Endocrine Rev. 28:20.
  9. LeRoith, D. and S. Yakar (2007) Nat. Clin. Pract. Endocrinol. Metab. 3:302.
  10. Denley, A. et al. (2005) Cytokine Growth Factor Rev. 16:421.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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IGF-I/IGF-1
Antibody (126002) [Un ...
MAB791
IGF-I/IGF-1 Antibody (126002) [Unconjugated]
Species: Mu
Applications: WB, B/N, ELISA(Cap)

Publications for IGF-I/IGF-1 Antibody (MAB791)(7)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 2 applications: ELISA Development, ELISA Development (Capture).


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ELISA Development
(4)
ELISA Development (Capture)
(1)
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(5)
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Showing Publications 1 - 7 of 7.
Publications using MAB791 Applications Species
PHIL-DONG MOON, MIN-HO KIM, HYUN-A OH, SUN-YOUNG NAM, NA-RA HAN, HYUN-JA JEONG et al. Cysteine induces longitudinal bone growth in mice by upregulating IGF-I International Journal of Molecular Medicine 2015-08-01 [PMID: 26101100]
Steger J, Fuller E, Garcia-Cuellar M, Hetzner K, Slany R Insulin-like growth factor 1 is a direct HOXA9 target important for hematopoietic transformation. Leukemia, 2014-09-25;29(4):901-8. 2014-09-25 [PMID: 25252870] (ELISA Development, Mouse) ELISA Development Mouse
Fletcher L, Isgor E, Sprague S, Williams L, Alajajian B, Jimenez D, Digicaylioglu M Spatial distribution of insulin-like growth factor binding protein-2 following hypoxic-ischemic injury. BMC Neurosci, 2013-12-21;14(0):158. 2013-12-21 [PMID: 24359611] (ELISA Development (Capture), Mouse) ELISA Development (Capture) Mouse
Jason M Fritz, Lori D Dwyer-Nield, Alvin M Malkinson Stimulation of neoplastic mouse lung cell proliferation by alveolar macrophage-derived, insulin-like growth factor-1 can be blocked by inhibiting MEK and PI3K activation Molecular Cancer 2011-12-01 [PMID: 21699731]
Mehta HH, Gao Q, Galet C IGFBP-3 is a metastasis suppression gene in prostate cancer. Cancer Res., 2011-06-22;71(15):5154-63. 2011-06-22 [PMID: 21697285] (ELISA Development, Mouse) ELISA Development Mouse
Yamada PM, Mehta HH, Hwang D Evidence of a role for insulin-like growth factor binding protein (IGFBP)-3 in metabolic regulation. Endocrinology, 2010-10-06;151(12):5741-50. 2010-10-06 [PMID: 20926583] (ELISA Development, Mouse) ELISA Development Mouse
Raina K, Blouin MJ, Singh RP, Majeed N, Deep G, Varghese L, Glode LM, Greenberg NM, Hwang D, Cohen P, Pollak MN, Agarwal R Dietary feeding of silibinin inhibits prostate tumor growth and progression in transgenic adenocarcinoma of the mouse prostate model. Cancer Res., 2007-11-15;67(22):11083-91. 2007-11-15 [PMID: 18006855] (ELISA Development, Mouse) ELISA Development Mouse

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Bioinformatics

Gene Symbol IGF1
Uniprot