Human LC3A DuoSet ELISA, 5 Plate Summary
Assay Type |
Solid Phase Sandwich ELISA |
Inter-Assay |
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Intra-Assay |
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Spike Recovery |
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Sample Volume |
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Applications/Dilutions
Dilutions |
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Application Notes |
No significant interference observed with available related molecules. |
Packaging, Storage & Formulations
Storage |
Store the unopened product at 2 - 8 °C. Do not use past expiration date. |
Notes
This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.
Alternate Names for Human LC3A DuoSet ELISA, 5 Plate
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Product General Protocols
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FAQs for LC3A (DY8558-05). (Showing 1 - 1 of 1 FAQ).
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May we ask if it is possible to perform IF to stain LC3-I and LC3-II separately with two different fluorescent colors?
- Yes, it is possible to perform IF stain for LC3-I and LC3-II separately with two different fluorescent colors! You will have to use two different primary antibodies and in order to avoid any potential background/cross reactivity issues, I would suggest that you employ conjugated primary antibodies for the testing. 1. Our LC3I antibody (NBP1-78964) has been designed to specifically detect the cytosolic form of the LC3 protein which is actually LC3 I (Note: LC3-II binds to the autophagic membranes). 2. There is not even a single antibody to our knowledge that would exclusively detect the LC3 II form, and you would have to detect LC3II/ autophagic membranes form with an antibody which detects LC3 I/LC3II together. Therefore you may opt second antibody from one of the followings: LC3 Antibody (NB100-2220), LC3 Antibody (NB100-2331), LC3 Antibody (NBP1-19167). All of these mentioned catalog #s come with different options for their conjugated forms and you may select appropriate conjugated forms for performing the IF staining using our explained criteria.