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Human IL-12 p70 Quantikine ELISA Kit

 (73 Publications)
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Product Details

Summary
Reactivity HuSpecies Glossary
Applications ELISA
Conjugate
HRP

Order Details

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Catalog# & Conjugate Size Price
HRP
PD1200
HRP
S1200
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Human IL-12 p70 Quantikine ELISA Kit Summary

Background
The Quantikine Human IL-12 Immunoassay is a 3.5 or 4.5 hour solid phase ELISA designed to measure IL-12 in cell culture supernates, serum, plasma, and urine. It contains Sf 21-expressed recombinant human IL-12 and antibodies raised against the recombinant factor. This immunoassay has been shown to quantitate recombinant human IL-12 accurately. Results obtained using natural human IL...-12 showed linear curves that were parallel to the standard curves obtained using the recombinant kit standards. These results indicate that this kit can be used to determine relative mass values for natural human IL-12.
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Specificity
Natural and recombinant human IL-12p70
Source
N/A
Assay Type
Solid Phase Sandwich ELISA
Inter-Assay
See PDF Datasheet for details
Intra-Assay
See PDF Datasheet for details
Spike Recovery
See PDF Datasheet for details
Sample Volume
See PDF Datasheet for details
Gene
IL12A

Applications/Dilutions

Dilutions
  • ELISA
Application Notes
No significant interference observed with available related molecules.
Publications
Read Publications using D1200.

Packaging, Storage & Formulations

Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Human IL-12 p70 Quantikine ELISA Kit

  • CLMF p35
  • Cytotoxic lymphocyte maturation factor 35 kDa subunit
  • IL12 p70
  • IL-12 p70
  • IL-12 subunit p35
  • IL-12, subunit p35
  • interleukin 12, p35
  • interleukin 12A (natural killer cell stimulatory factor 1, cytotoxic lymphocytematuration factor 1, p35)
  • interleukin-12 alpha chain
  • interleukin-12 subunit alpha
  • natural killer cell stimulatory factor 1, 35 kD subunit
  • NF cell stimulatory factor chain 1
  • NK cell stimulatory factor chain 1
  • NKSF1
  • p35

Background

Interleukin 12 (IL-12), also known as natural killer cell stimulatory factor (NKSF) or cytotoxic lymphocyte maturation factor (CLMF), is a heterodimeric pleiotropic cytokine made up of a 40 kDa (p40) subunit and a 35 kDa (p35) subunit. The IL-12 p40 subunit is shared by IL-23, another heterodimeric cytokine that has biological activities similar to, as well as distinct from, IL-12. IL-12 is produced by macrophages and B cells and has been shown to have multiple effects on T cells and natural killer (NK) cells. While mouse IL-12 is active on both human and mouse cells, human IL-12 is not active on mouse cells.

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Publications for IL-12 p70/IL-12A (D1200)(73)

We have publications tested in 3 confirmed species: Human, Mouse, Primate - Pan troglodytes (Chimpanzee).


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Human
(71)
Mouse
(1)
Primate - Pan troglodytes (Chimpanzee)
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Showing Publications 1 - 10 of 73. Show All 73 Publications.
Publications using D1200 Applications Species
Scovino, AM;Dahab, EC;Diniz-Lima, I;de Senna Silveira, E;Barroso, SPC;Cardoso, KM;Nico, D;Makhoul, GJ;da Silva-Junior, EB;Freire-de-Lima, CG;Freire-de-Lima, L;Fonseca, LMD;Valente, N;Nacife, V;Machado, A;Araújo, M;Vieira, GF;Pauvolid-Corrêa, A;Siqueira, M;Morrot, A; A Comparative Analysis of Innate Immune Responses and the Structural Characterization of Spike from SARS-CoV-2 Gamma Variants and Subvariants Microorganisms 2024-04-02 [PMID: 38674664] (Human) Human
Nosik, M;Belikova, MG;Ryzhov, K;Avdoshina, D;Sobkin, A;Zverev, V;Svitich, O; Unique Profile of Proinflammatory Cytokines in Plasma of Drug-Naïve Individuals with Advanced HIV/TB Co-Infection Viruses 2023-06-06 [PMID: 37376629] (Human) Human
H Zheng, X Yu, ML Ibrahim, D Foresman, M Xie, JO Johnson, TA Boyle, B Ruffell, BA Perez, SJ Antonia, N Ready, AN Saltos, MJ Cantwell, AA Beg Combination IFNbeta and membrane-stable CD40L maximize tumor dendritic cell activation and lymph node trafficking to elicit systemic T-cell immunity Cancer Immunology Research, 2023-04-03;0(0):. 2023-04-03 [PMID: 36757308] (Human) Human
SY Cho, SM Jeong, YJ Jeon, SJ Yang, JE Hwang, BM Yoo, HS Kim WT1 Pulsed Human CD141+ Dendritic Cell Vaccine Has High Potential in Solid Tumor-Targeted Immunotherapy International Journal of Molecular Sciences, 2023-01-12;24(2):. 2023-01-12 [PMID: 36675017] (Human) Human
J Milosevic, M Jurisevic, V Grbovic, I Jovanovic, N Gajovic, A Jurisic-Sk Potential Protective Role of Galectin-3 in Patients with Gonarthrosis and Diabetes Mellitus: A Cross-Sectional Study International Journal of Environmental Research and Public Health, 2022-09-13;19(18):. 2022-09-13 [PMID: 36141752] (Human) Human
N Jirapongwa, S Thongchot, W Chiraphapp, T Chieochans, D Sa-Nguanra, M Warnnissor, P Thuwajit, PT Yenchitsom, C Thuwajit Mesothelin?specific T cell cytotoxicity against triple negative breast cancer is enhanced by 40s ribosomal protein subunit 3?treated self?differentiated dendritic cells Oncology reports, 2022-05-26;48(1):. 2022-05-26 [PMID: 35616135] (Human) Human
DH Lee, HK Park, HR Lee, H Sohn, S Sim, HJ Park, YS Shin, YK Kim, Y Choi, HS Park Immunoregulatory effects of Lactococcus lactis-derived extracellular vesicles in allergic asthma Clinical and translational allergy, 2022-03-01;12(3):e12138. 2022-03-01 [PMID: 35344296] (Human) Human
A Weber, A Schwiebs, H Solhaug, J Stenvik, AM Nilsen, M Wagner, B Relja, HH Radeke Nanoplastics affect the inflammatory cytokine release by primary human monocytes and dendritic cells Environment international, 2022-03-15;163(0):107173. 2022-03-15 [PMID: 35303527] (Human) Human
B Liu, Y Cao, Y Li, H Ma, M Yang, Q Zhang, G Li, K Zhang, Y Wu, Y Zhou, W Yang, T Sun Glioma Stem Cells Upregulate CD39 Expression to Escape Immune Response through SOX2 Modulation Cancers, 2022-02-03;14(3):. 2022-02-03 [PMID: 35159053] (Human) Human
S Kos, M Bosnjak, T Jesenko, B Markelc, U Kamensek, K Znidar, U Matkovic, A Rencelj, G Sersa, R Hudej, A Tuljak, M Peterka, M Cemazar Non-Clinical In Vitro Evaluation of Antibiotic Resistance Gene-Free Plasmids Encoding Human or Murine IL-12 Intended for First-in-Human Clinical Study Pharmaceutics, 2021-10-19;13(10):. 2021-10-19 [PMID: 34684032] (Human) Human
Show All 73 Publications.

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Bioinformatics

Gene Symbol IL12A