Mouse HSF2 ELISA Kit (Colorimetric)

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ELISA: Mouse HSF2 ELISA Kit (Colorimetric) [NBP2-76592] - Samples were spiked with high concentrations of Mouse HSF2 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range ...read more
ELISA: Mouse HSF2 ELISA Kit (Colorimetric) [NBP2-76592] - Standard Curve Reference

Product Details

Summary
Reactivity MuSpecies Glossary
Applications ELISA
Suitable Sample Type
Serum, plasma and other biological fluids
Standard Curve Range
7.813 - 500pg/mL
Sensitivity
4.688pg/mL

Order Details

Mouse HSF2 ELISA Kit (Colorimetric) Summary

Specificity
This kit recognizes Mouse HSF2 in samples. No significant cross-reactivity or interference between Mouse HSF2 and analogues was observed.
Standard Curve Range
7.813 - 500pg/mL
Sensitivity
4.688pg/mL
Assay Type
Sandwich-ELISA
Inter-Assay
CV% < 4.9
Intra-Assay
CV% < 5.02
Spike Recovery
86-105%
Sample Volume
100 uL
Kit Type
ELISA Kit (Colorimetric)
Gene
HSF2

Applications/Dilutions

Dilutions
  • ELISA

Packaging, Storage & Formulations

Storage
Storage of components varies. See protocol for specific instructions.

Kit Components

Components
  1. Biotinylated Detection Ab Diluent
  2. Concentrated Biotinylated Detection Ab (100x)
  3. Concentrated HRP Conjugate (100x)
  4. Concentrated Wash Buffer (25x)
  5. HRP Conjugate Diluent
  6. Micro ELISA Plate (Dismountable)
  7. Plate Sealer
  8. Product Manual
  9. Reference Standard
  10. Sample Diluent
  11. Stop Solution
  12. Substrate Reagent

Alternate Names for Mouse HSF2 ELISA Kit (Colorimetric)

  • heat shock factor 2
  • heat shock factor protein 2
  • heat shock transcription factor 2MGC75048
  • HSF 2
  • HSF2
  • HSTF 2
  • HSTF2
  • MGC117376
  • MGC156196

Background

Heat shock factor 1 (HSF1) is a heat shock transcription factor that activates the transcription of genes encoding products required for protein folding, processing, targeting, degradation, and function. Up-regulation of expression of heat shock proteins in response to stress occurs at the level of transcription through a heat shock element and a HSF transcription factor. Amino acid sequences for most HSFs are highly conserved. A DNA binding domain is at the N-terminus, hydrophobic repeats (essential to the formation of active trimers) are adjacent to this binding domain, and another short hydrophobic repeat (necessary for suppression of trimerization) occurs toward the C-terminus. HSF2 exists as two isoforms, the alpha form being more transcriptionally active than the smaller beta form. Various experiments have suggested that HSF2 may play roles in differentiation and development.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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Bioinformatics

Gene Symbol HSF2