![Western Blot: HLA DRB1 Antibody (SPM289) - Azide and BSA Free [NBP2-34756] - Western Blot Analysis of Ramos cells and human spleen tissue lysate using HLA DRB1 Antibody (SPM289).](http://images.novusbio.com/fullsize/HLA-DRB1-Antibody-SPM289-Azide-and-BSA-Free-Western-Blot-NBP2-34756-img0005.jpg "Western Blot: HLA DRB1 Antibody (SPM289) - Azide and BSA Free [NBP2-34756] - Western Blot Analysis of Ramos cells and human spleen tissue lysate using HLA DRB1 Antibody (SPM289).")
| Reactivity | HuSpecies Glossary |
| Applications | WB, Flow, ICC/IF, IHC, CyTOF-ready |
| Clone | SPM289 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Format | Azide and BSA Free |
| Concentration | 1.0 mg/ml |
| Description | 1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP2-45311). Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C. |
| Immunogen | Activated human peripheral blood mononuclear cells |
| Localization | Cell surface |
| Specificity | This monoclonal antibody reacts with the beta-chain of HLA-DR antigen, a member of MHC class II molecules. It does not cross react with HLA-DP and HLA-DQ. The L243 antibody recognizes a different epitope than the SPM289 monoclonal antibody, and these antibodies do not cross-block binding to each others respective epitopes. HLA-DR is a heterodimeric cell surface glycoprotein comprised of a 36kDa alpha (heavy) chain and a 28kDa beta (light) chain. It is expressed on B-cells, activated T-cells, monocytes/macrophages, dendritic cells and other non-professional APCs. In conjunction with the CD3/TCR complex and CD4 molecules, HLA-DR is critical for efficient peptide presentation to CD4+ T cells. It is an excellent histiocytic marker in paraffin sections producing intense staining. True histiocytic neoplasms are similarly positive. HLA-DR antigens also occur on a variety of epithelial cells and their corresponding neoplastic counterparts. |
| Isotype | IgG2b Kappa |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | HLA-DRB1 |
| Purity | Protein A or G purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes. Optimal dilution for a specific application should be determined. |
| Storage | Store at -20 to -80C. Avoid freeze-thaw cycles. |
| Buffer | 10 mM PBS |
| Preservative | No Preservative |
| Concentration | 1.0 mg/ml |
| Purity | Protein A or G purified |
Secondary Antibodies |
Isotype Controls |
Research Areas for HLA-DR Antibody (NBP2-34756)Find related products by research area.
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| Gene Symbol | HLA-DRB1 |
![Immunohistochemistry-Paraffin: HLA DRB1 Antibody (SPM289) - Azide and BSA Free [NBP2-34756] - Formalin-paraffin human tonsil stained with HLA-DR MAb (SPM289).](http://images.novusbio.com/fullsize/HLA-DRB1-Antibody-SPM289-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-34756-img0002.jpg "Immunohistochemistry-Paraffin: HLA DRB1 Antibody (SPM289) - Azide and BSA Free [NBP2-34756] - Formalin-paraffin human tonsil stained with HLA-DR MAb (SPM289).")
![Flow Cytometry: HLA DRB1 Antibody (SPM289) - Azide and BSA Free [NBP2-34756] - Flow Cytometric Analysis of human Raji cells using HLA DRB1 Antibody (SPM289).followed by Goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).](http://images.novusbio.com/fullsize/HLA-DRB1-Antibody-SPM289-Azide-and-BSA-Free-Flow-Cytometry-NBP2-34756-img0003.jpg "Flow Cytometry: HLA DRB1 Antibody (SPM289) - Azide and BSA Free [NBP2-34756] - Flow Cytometric Analysis of human Raji cells using HLA DRB1 Antibody (SPM289).followed by Goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).")
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