We are interested in custom labeling our antibodies to be able to integrate new markers into already existing multicolor FACS panels. We would be glad to receive pricing and general order information for anti-HLA-DRB1 and anti-HLA-DRB5. To estimate expenses for future antibody labeling orders it would be helpful to know on what basis (amount, type, etc.) the price is going to be calculated. Right now the antibodies are still in mouse ascites. Would it be possible to integrate the elution of the antibodies from the ascites before labeling as part of the service agreement?

Name: HLA DRB1 Antibody (DA2) [Janelia Fluor&#174; 669]
Brand: Novus Biologicals
SKU: NB100-2705JF669
Price: 499 USD

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Accepted Answer

While we do not generally do this kind of custom purification unless it is for very large amounts of antibody, we do have a number of kits available that may be of use to you. Since an antibody that is still in ascites can not be labeled, it will need to be purified. A list of our kits available for this can be found at the following link: AbSelect. Once the antibodies are purified, they can be labeled with any number of different colors. A list of these kits for labeling can be found at the following link: Lightning-Link.

At	=	A. thaliana	SyHa	=	Golden Syrian Hamster
All	=	All Species	Ha	=	Hamster
An	=	Animal	Hu	=	Human
ArHa	=	Armenian Hamster	I	=	Insect
Av	=	Avian	Ll	=	Llama
Bb	=	Baboon	Ma	=	Mammal
Ba	=	Bacteria	Mk	=	Monkey
Bv	=	Bovine	Mu	=	Mouse
Ca	=	Canine	Multi	=	Multi-species
Ce	=	C. Elegans	NA	=	Non-species specific
Ch	=	Chicken	Or	=	Orangutan
ChHa	=	Chinese Hamster	Pl	=	Plant
Cm	=	Cynomolgus Monkey	Pm	=	Primate
Do	=	Donkey	Po	=	Porcine
Dr	=	Drosophilia	Rb	=	Rabbit
Ec	=	E. Coli	Rt	=	Rat
Eq	=	Equine	RM	=	Rhesus Macaque
Fe	=	Feline	Sh	=	Sheep
Ft	=	Ferret	Vb	=	Vertebrate
Fi	=	Fish	Vi	=	Virus
Fu	=	Fungi	Xp	=	Xenopus
Ge	=	Gerbil	Ye	=	Yeast
GP	=	Guinea Pig	Ze	=	Zebrafish
Gt	=	Goat

Reactivity	HuSpecies Glossary
Applications	WB, ELISA, Flow, IHC, CyTOF-ready
Clone	DA2
Clonality	Monoclonal
Host	Mouse
Conjugate	Janelia Fluor 669

Catalog# & Conjugate	Size	Price
Alexa Fluor 350 NB100-2705AF350

Alexa Fluor 405 NB100-2705AF405

Alexa Fluor 488 NB100-2705AF488

Alexa Fluor 532 NB100-2705AF532

Alexa Fluor 594 NB100-2705AF594

Alexa Fluor 647 NB100-2705AF647

Alexa Fluor 700 NB100-2705AF700

Alexa Fluor 750 NB100-2705AF750

Allophycocyanin NB100-2705APC

Allophycocyanin/Cy7 NB100-2705APCCY7

Biotin NB100-2705B

CoraFluor 1 NB100-2705CL1

DyLight 350 NB100-2705UV

DyLight 405 NB100-2705V

DyLight 488 NB100-2705G

DyLight 550 NB100-2705R

DyLight 594 NB100-2705DL594

DyLight 650 NB100-2705C

DyLight 680 NB100-2705FR

DyLight 755 NB100-2705IR

FITC NB100-2705F

HRP NB100-2705H

Janelia Fluor 525 NB100-2705JF525

Janelia Fluor 549 NB100-2705JF549

Janelia Fluor 585 NB100-2705JF585

Janelia Fluor 635 NB100-2705JF635

Janelia Fluor 646 NB100-2705JF646

PE NB100-2705PE

PE/Atto594 NB100-2705PEATT594

PE/Cy5.5 NB100-2705PECY55

PE/Cy7 NB100-2705PECY7

PerCP NB100-2705PCP

Unconjugated NB100-2705

mFluor Violet 450 SE NB100-2705MFV450

mFluor Violet 500 SE NB100-2705MFV500

mFluor Violet 610 SE NB100-2705MFV610

Catalog# & Formulation	Size	Price
Standard Formulation NB100-2705

BSA Free NB100-2705

BSA Free NB100-2705-0.025mg

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NB100-2705AF350
Alexa Fluor 405	NB100-2705AF405
Alexa Fluor 488	NB100-2705AF488
Alexa Fluor 532	NB100-2705AF532
Alexa Fluor 594	NB100-2705AF594
Alexa Fluor 647	NB100-2705AF647
Alexa Fluor 700	NB100-2705AF700
Alexa Fluor 750	NB100-2705AF750
Allophycocyanin	NB100-2705APC
Allophycocyanin/Cy7	NB100-2705APCCY7
Biotin	NB100-2705B
CoraFluor 1	NB100-2705CL1
DyLight 350	NB100-2705UV
DyLight 405	NB100-2705V
DyLight 488	NB100-2705G
DyLight 550	NB100-2705R
DyLight 594	NB100-2705DL594
DyLight 650	NB100-2705C
DyLight 680	NB100-2705FR
DyLight 755	NB100-2705IR
FITC	NB100-2705F
HRP	NB100-2705H
Janelia Fluor 525	NB100-2705JF525
Janelia Fluor 549	NB100-2705JF549
Janelia Fluor 585	NB100-2705JF585
Janelia Fluor 635	NB100-2705JF635
Janelia Fluor 646	NB100-2705JF646
PE	NB100-2705PE
PE/Atto594	NB100-2705PEATT594
PE/Cy5.5	NB100-2705PECY55
PE/Cy7	NB100-2705PECY7
PerCP	NB100-2705PCP
Unconjugated	NB100-2705
mFluor Violet 450 SE	NB100-2705MFV450
mFluor Violet 500 SE	NB100-2705MFV500
mFluor Violet 610 SE	NB100-2705MFV610

HLA DRB1 Antibody (DA2) [Janelia Fluor® 669]

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Immunogen	The membranes of LKT cells (a B lymphoblastoid cell line)
Epitope	1st domain, monomorphic.
Localization	Cell Membrane
Specificity	DA2 will detect dimers and the beta chain by western blot. DA2 will bind all DR and DP antigens except DR7.
Isotype	IgG1
Clonality	Monoclonal
Host	Mouse
Gene	HLA-DRB1
Purity	Protein A purified
Innovator's Reward	Test in a species/application not listed above to receive a full credit towards a future purchase. Learn about the Innovator's Reward

Dilutions	CyTOF-ready ELISA Flow Cytometry Immunohistochemistry Western Blot
Application Notes	Optimal dilution of this antibody should be experimentally determined.

Storage	Store at 4C in the dark.
Buffer	50mM Sodium Borate
Preservative	0.05% Sodium Azide
Purity	Protein A purified