![Western Blot: Histone H2B Antibody [NBP2-42854] - Various whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with Histone H2B antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/Histone-H2B-Antibody-Western-Blot-NBP2-42854-img0008.jpg "Western Blot: Histone H2B Antibody [NBP2-42854] - Various whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with Histone H2B antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
| Reactivity | Hu, Mu, Ze, Rt, Po, BvSpecies Glossary |
| Applications | WB, ICC/IF, IHC |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Immunogen | Full length human Histone H2B Recombinant protein. |
| Predicted Species | Rat (98%), Porcine (99%), Bovine (99%). Backed by our 100% Guarantee. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | H2BC18 |
| Purity | Antigen Affinity-purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
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| Publications |
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| Storage | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Buffer | PBS, 1% BSA, 20% Glycerol |
| Preservative | 0.01% Thimerosal |
| Purity | Antigen Affinity-purified |
| Publication using NBP2-42854 | Applications | Species |
|---|---|---|
| Lyu Y, Yang Y, Talwar V et al Hypoxia-inducible factor 1 recruits FACT and RNF20/40 to mediate histone ubiquitination and transcriptional activation of target genes Cell Rep 2024-03-22 [PMID: 38517892] (WB, Human) | WB | Human |
Secondary Antibodies |
Isotype Controls |
Research Areas for Histone H2B Antibody (NBP2-42854)Find related products by research area.
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The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
| Gene Symbol | H2BC18 |
![Immunocytochemistry/Immunofluorescence: Histone H2B Antibody [NBP2-42854] - Analysis of Sample: HeLa cells were fixed in 4% paraformaldehyde/PBS for 15 min. Green: Histone H2B protein stained by Histone H2B antibody diluted at 1:500. Blue: Hoechst 33342 staining.](http://images.novusbio.com/fullsize/Histone-H2B-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-42854-img0004.jpg "Immunocytochemistry/Immunofluorescence: Histone H2B Antibody [NBP2-42854] - Analysis of Sample: HeLa cells were fixed in 4% paraformaldehyde/PBS for 15 min. Green: Histone H2B protein stained by Histone H2B antibody diluted at 1:500. Blue: Hoechst 33342 staining.")
![Immunohistochemistry-Paraffin: Histone H2B Antibody [NBP2-42854] - Analysis of mouse middle brain. Histone H2B antibody diluted at 1:500.](http://images.novusbio.com/fullsize/Histone-H2B-Antibody-Immunohistochemistry-Paraffin-NBP2-42854-img0002.jpg "Immunohistochemistry-Paraffin: Histone H2B Antibody [NBP2-42854] - Analysis of mouse middle brain. Histone H2B antibody diluted at 1:500.")
![Western Blot: Histone H2B Antibody [NBP2-42854] - Various whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with Histone H2B antibody diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/Histone-H2B-Antibody-Western-Blot-NBP2-42854-img0006.jpg "Western Blot: Histone H2B Antibody [NBP2-42854] - Various whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with Histone H2B antibody diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
![Immunohistochemistry-Paraffin: Histone H2B Antibody [NBP2-42854] - Analysis of H1299 xenograft, using Histone H2B antibody at 1:500 dilution.](http://images.novusbio.com/fullsize/Histone-H2B-Antibody-Immunohistochemistry-Paraffin-NBP2-42854-img0001.jpg "Immunohistochemistry-Paraffin: Histone H2B Antibody [NBP2-42854] - Analysis of H1299 xenograft, using Histone H2B antibody at 1:500 dilution.")
![Western Blot: Histone H2B Antibody [NBP2-42854] -The equivalent of 4 larvae was loadedA: 120hpf larvae4-12% NuPAGE Bis Tris precast gel diluted at 1:1000](http://images.novusbio.com/fullsize/antibody/nbp2-42854_rabbit-polyclonal-histone-h2b-antibody-western-blot-9820238527..jpg "Western Blot: Histone H2B Antibody [NBP2-42854] -The equivalent of 4 larvae was loadedA: 120hpf larvae4-12% NuPAGE Bis Tris precast gel diluted at 1:1000")
![Immunohistochemistry PITX2 Antibody [Unconjugated]](https://images.novusbio.com/images2/PITX2_AF7388_Immunohistochemistry_12162.jpg)
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![N/A Kallikrein 3/PSA [HRP]](https://images.novusbio.com/images2/DATA_Kallikrein_3_DKK300_ELISA_730.jpg)
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![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")