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![Western Blot: Histone H2B Antibody (SD20-63) [NBP2-67753] - Analysis of Histone H2B on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.Positive control: Lane 1: Hela cell lysateLane 2: PC-12 cell lysate](http://images.novusbio.com/fullsize/Histone-H2B-Antibody-SD20-63-Western-Blot-NBP2-67753-img0009.jpg "Western Blot: Histone H2B Antibody (SD20-63) [NBP2-67753] - Analysis of Histone H2B on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.Positive control: Lane 1: Hela cell lysateLane 2: PC-12 cell lysate")
![Immunocytochemistry/Immunofluorescence: Histone H2B Antibody (SD20-63) [NBP2-67753] - Staining Histone H2B in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/Histone-H2B-Antibody-SD20-63-Immunocytochemistry-Immunofluorescence-NBP2-67753-img0002.jpg)
![Immunohistochemistry-Paraffin: Histone H2B Antibody (SD20-63) [NBP2-67753] - Analysis of paraffin-embedded mouse testis tissue using anti-Histone H2B antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Histone-H2B-Antibody-SD20-63-Immunohistochemistry-Paraffin-NBP2-67753-img0008.jpg)
![Flow Cytometry: Histone H2B Antibody (SD20-63) [NBP2-67753] - Analysis of Hela cells with Histone H2B antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.](http://images.novusbio.com/fullsize/Histone-H2B-Antibody-SD20-63-Flow-Cytometry-NBP2-67753-img0001.jpg)
![Immunohistochemistry-Paraffin: Histone H2B Antibody (SD20-63) [NBP2-67753] - Analysis of paraffin-embedded human breast carcinoma tissue using anti-Histone H2B antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Histone-H2B-Antibody-SD20-63-Immunohistochemistry-Paraffin-NBP2-67753-img0003.jpg)
![Immunohistochemistry-Paraffin: Histone H2B Antibody (SD20-63) [NBP2-67753] - Analysis of paraffin-embedded human liver tissue using anti-Histone H2B antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Histone-H2B-Antibody-SD20-63-Immunohistochemistry-Paraffin-NBP2-67753-img0004.jpg)
![Immunohistochemistry-Paraffin: Histone H2B Antibody (SD20-63) [NBP2-67753] - Analysis of paraffin-embedded human tonsil tissue using anti-Histone H2B antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Histone-H2B-Antibody-SD20-63-Immunohistochemistry-Paraffin-NBP2-67753-img0005.jpg)
![Immunohistochemistry-Paraffin: Histone H2B Antibody (SD20-63) [NBP2-67753] - Analysis of paraffin-embedded mouse colon tissue using anti-Histone H2B antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Histone-H2B-Antibody-SD20-63-Immunohistochemistry-Paraffin-NBP2-67753-img0006.jpg)
![Immunohistochemistry-Paraffin: Histone H2B Antibody (SD20-63) [NBP2-67753] - Analysis of paraffin-embedded mouse liver tissue using anti-Histone H2B antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Histone-H2B-Antibody-SD20-63-Immunohistochemistry-Paraffin-NBP2-67753-img0007.jpg)
![Western Blot: Histone H2B Antibody [NBP2-42854] - Various whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with Histone H2B antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/Histone-H2B-Antibody-Western-Blot-NBP2-42854-img0008.jpg "Western Blot: Histone H2B Antibody [NBP2-42854] - Various whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with Histone H2B antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
![Immunocytochemistry/Immunofluorescence: Histone H2B Antibody [NBP2-42854] - Analysis of Sample: HeLa cells were fixed in 4% paraformaldehyde/PBS for 15 min. Green: Histone H2B protein stained by Histone H2B antibody diluted at 1:500. Blue: Hoechst 33342 staining.](http://images.novusbio.com/fullsize/Histone-H2B-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-42854-img0004.jpg)
![Immunohistochemistry-Paraffin: Histone H2B Antibody [NBP2-42854] - Analysis of mouse middle brain. Histone H2B antibody diluted at 1:500.](http://images.novusbio.com/fullsize/Histone-H2B-Antibody-Immunohistochemistry-Paraffin-NBP2-42854-img0002.jpg)
![Western Blot: Histone H2B Antibody [NBP2-42854] - Various whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with Histone H2B antibody diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/Histone-H2B-Antibody-Western-Blot-NBP2-42854-img0006.jpg)
![Immunohistochemistry-Paraffin: Histone H2B Antibody [NBP2-42854] - Analysis of H1299 xenograft, using Histone H2B antibody at 1:500 dilution.](http://images.novusbio.com/fullsize/Histone-H2B-Antibody-Immunohistochemistry-Paraffin-NBP2-42854-img0001.jpg)
![Western Blot: Histone H2B Antibody [NBP2-42854] -The equivalent of 4 larvae was loadedA: 120hpf larvae4-12% NuPAGE Bis Tris precast gel diluted at 1:1000](http://images.novusbio.com/fullsize/antibody/nbp2-42854_rabbit-polyclonal-histone-h2b-antibody-western-blot-9820238527..jpg)
![Western Blot: Histone H2B Antibody (3U2P5) [NBP3-15598] - Western blot analysis of extracts of various cell lines, using Histone H2B antibody (NBP3-15598) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 60s.](http://images.novusbio.com/fullsize/Histone-H2B-Antibody-3U2P5-Western-Blot-NBP3-15598-img0004.jpg "Western Blot: Histone H2B Antibody (3U2P5) [NBP3-15598] - Western blot analysis of extracts of various cell lines, using Histone H2B antibody (NBP3-15598) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 60s.")
![Immunohistochemistry: Histone H2B Antibody (3U2P5) [Histone H2B] - Immunohistochemistry analysis of paraffin-embedded Mouse brain tissue using Histone H2B Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15598_rabbit-histone-h2b-mab-3u2p5-5220251734514.jpg)
![Immunohistochemistry: Histone H2B Antibody (3U2P5) [Histone H2B] - Immunohistochemistry analysis of paraffin-embedded Human spleen tissue using Histone H2B Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15598_rabbit-histone-h2b-mab-3u2p5-52202517344251.jpg)
![Immunohistochemistry: Histone H2B Antibody (3U2P5) [Histone H2B] - Immunohistochemistry analysis of paraffin-embedded Rat kidney tissue using Histone H2B Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15598_rabbit-histone-h2b-mab-3u2p5-5220251733599.jpg)
![Immunohistochemistry: Histone H2B Antibody (3U2P5) [Histone H2B] - Immunohistochemistry analysis of paraffin-embedded Human cervix cancer tissue using Histone H2B Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15598_rabbit-histone-h2b-mab-3u2p5-52202517361297.jpg)
![Immunohistochemistry: Histone H2B Antibody (3U2P5) [Histone H2B] - Immunohistochemistry analysis of paraffin-embedded Rat brain tissue using Histone H2B Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15598_rabbit-histone-h2b-mab-3u2p5-52202517333863.jpg)
![Immunohistochemistry: Histone H2B Antibody (3U2P5) [Histone H2B] - Immunohistochemistry analysis of paraffin-embedded Human liver tissue using Histone H2B Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15598_rabbit-histone-h2b-mab-3u2p5-52202517352776.jpg)
![Western Blot: Histone H2B Antibody (1B3F12/A9) [NBP2-54770]](http://images.novusbio.com/fullsize/Histone-H2B-Antibody-1B3F12-A9-Western-Blot-NBP2-54770-img0001.jpg "Western Blot: Histone H2B Antibody (1B3F12/A9) [NBP2-54770]")
![Chromatin Immunoprecipitation: Histone H2B Antibody (1B3F12/A9) [NBP2-54770]](http://images.novusbio.com/fullsize/Histone-H2B-Antibody-1B3F12-A9-Chromatin-Immunoprecipitation-ChIP-NBP2-54770-img0004.jpg)
![ELISA: Histone H2B Antibody (1B3F12/A9) [NBP2-54770]](http://images.novusbio.com/fullsize/Histone-H2B-Antibody-1B3F12-A9-ELISA-NBP2-54770-img0003.jpg)
![Western Blot: Histone H2B Antibody [NBP2-59270] - Western blot was performed on whole cell extracts from HeLa cells (40 ug, lane 1) and on 1 ug of recombinant histone H2B (lane 2) using the antibody against H2B. The antibody was diluted 1:10,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (kDa) is shown on the left.](http://images.novusbio.com/fullsize/Histone-H2B-Antibody-Western-Blot-NBP2-59270-img0002.jpg "Western Blot: Histone H2B Antibody [NBP2-59270] - Western blot was performed on whole cell extracts from HeLa cells (40 ug, lane 1) and on 1 ug of recombinant histone H2B (lane 2) using the antibody against H2B. The antibody was diluted 1:10,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (kDa) is shown on the left.")
![Immunocytochemistry/Immunofluorescence: Histone H2B Antibody [NBP2-59270] - HeLa cells were stained with the antibody against H2B and with DAPI. Cells were fixed with 4% formaldehyde for 10 minutes and blocked with PBS/TX-100 containing 1% BSA. The cells were immunofluorescently labeled with the H2B antibody (middle) diluted 1:1,000 in blocking solution followed by an anti-rabbit antibody conjugated to Alexa Fluor 488. The left panel shows staining of the nuclei with DAPI. A merge of the two stainings is shown on the right.](http://images.novusbio.com/fullsize/Histone-H2B-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-59270-img0001.jpg)
![Chromatin Immunoprecipitation: Histone H2B Antibody [NBP2-59270] - ChIP assays were performed using human HeLa cells, the antibody against H2B and optimized PCR primer sets for qPCR. ChIP was performed with a ChIP-seq kit on sheared chromatin from 1 million cells. A titration of the antibody consisting of 0.5, 1, 2 and 5 ug per ChIP experiment was analysed. IgG (2 ug/IP) was used as negative IP control. QPCR was performed with primers for the promoters of the active GAPDH and EIF4A2 genes, used as negative controls and for the inactive MYOD1 gene and the Sat2 satellite repeat, used as positive controls. Figure shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis.](http://images.novusbio.com/fullsize/Histone-H2B-Antibody-Chromatin-Immunoprecipitation-NBP2-59270-img0004.jpg)
![ELISA: Histone H2B Antibody [NBP2-59270] - To determine the titer of the antibody, an ELISA was performed using a serial dilution of the antibody directed against H2B in antigen coated wells. By plotting the absorbance against the antibody dilution, the titer of the antibody was estimated to be 1:40,000.](http://images.novusbio.com/fullsize/Histone-H2B-Antibody-ELISA-NBP2-59270-img0003.jpg)
![Western Blot: Histone H2B [ac Lys15] Antibody [NBP2-59272] - Western blot was performed on whole cell (25 ug, lane 1) and histone extracts (15 ug, lane 2) from HeLa cells, and on 1 ug of recombinant histone H2A, H2B, H3 and H4 (lane 3, 4, 5 and 6, respectively) using the antibody against H2BK15ac. The antibody was diluted 1:500 in TBS-Tween containing 5% skimmed milk. The marker (kDa) is shown on the left.](http://images.novusbio.com/fullsize/Histone-H2B-Antibody-Western-Blot-NBP2-59272-img0001.jpg "Western Blot: Histone H2B [ac Lys15] Antibody [NBP2-59272] - Western blot was performed on whole cell (25 ug, lane 1) and histone extracts (15 ug, lane 2) from HeLa cells, and on 1 ug of recombinant histone H2A, H2B, H3 and H4 (lane 3, 4, 5 and 6, respectively) using the antibody against H2BK15ac. The antibody was diluted 1:500 in TBS-Tween containing 5% skimmed milk. The marker (kDa) is shown on the left.")
![Immunocytochemistry/Immunofluorescence: Histone H2B [ac Lys15] Antibody [NBP2-59272] - HeLa cells were stained with the antibody against H2BK15ac and with DAPI. Cells were fixed with 4% formaldehyde for 10' and blocked with PBS/ TX-100 containing 5% normal goat serum and 1% BSA. The cells were immunofluorescently labeled with the H2BK15ac antibody (left) diluted 1:500 in blocking solution followed by an anti-rabbit antibody conjugated to Alexa Fluor 488. The middle panel shows staining of the nuclei with DAPI. A merge of the two stainings is shown on the right.](http://images.novusbio.com/fullsize/Histone-H2B-Antibody-Immunofluorescence-NBP2-59272-img0004.jpg)
![Chromatin Immunoprecipitation: Histone H2B [ac Lys15] Antibody [NBP2-59272] - ChIP assays were performed using human HeLa cells, the antibody against H2BK15ac and optimized PCR primer sets for qPCR. ChIP was performed using sheared chromatin from 1.5 million cells. A titration of the antibody consisting of 0.5, 1, 2 and, 5 ug per ChIP experiment was analysed. IgG (1 ug/IP) was used as negative IP control. QPCR was performed with primers for a region approximately 1 kb upstream of the GAPDH and ACTB promoters, used as positive controls, and for the coding region of the inactive MYOD1 gene and the Sat2 satellite repeat, used as negative controls. Figure shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).](http://images.novusbio.com/fullsize/Histone-H2B-Antibody-Chromatin-Immunoprecipitation-NBP2-59272-img0003.jpg)
![ELISA: Histone H2B [ac Lys15] Antibody [NBP2-59272] - To determine the titer of the antibody, an ELISA was performed using a serial dilution of the antibody directed against H2BK15ac in antigen coated wells. The antigen used was a peptide containing the histone modification of interest. By plotting the absorbance against the antibody dilution, the titer of the antibody was estimated to be 1:29,700.](http://images.novusbio.com/fullsize/Histone-H2B-Antibody-ELISA-NBP2-59272-img0002.jpg)
![Western Blot: Histone H2B [ac Lys12] Antibody [NBP2-59271] - Histone H2B Antibody [NBP2-59271] - Western blot was performed on whole cell (25 ug, lane 1) and histone extracts (15 ug, lane 2) from HeLa cells, and on 1 ug of recombinant histone H2A, H2B, H3 and H4 (lane 3, 4, 5 and 6, respectively) using the antibody against H2BK12ac. The antibody was diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The marker (kDa) is shown on the left.](http://images.novusbio.com/fullsize/Histone-H2B-Antibody-Western-Blot-NBP2-59271-img0001.jpg "Western Blot: Histone H2B [ac Lys12] Antibody [NBP2-59271] - Histone H2B Antibody [NBP2-59271] - Western blot was performed on whole cell (25 ug, lane 1) and histone extracts (15 ug, lane 2) from HeLa cells, and on 1 ug of recombinant histone H2A, H2B, H3 and H4 (lane 3, 4, 5 and 6, respectively) using the antibody against H2BK12ac. The antibody was diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The marker (kDa) is shown on the left.")
![Chromatin Immunoprecipitation: Histone H2B [ac Lys12] Antibody [NBP2-59271] - Histone H2B Antibody [NBP2-59271] - ChIP assays were performed using human HeLa cells, the antibody against H2BK12ac and optimized PCR primer sets for qPCR. ChIP was performed using sheared chromatin from 1.5 million cells. A titration of the antibody consisting of 0.5, 1, 2 and, 5 ug per ChIP experiment was analysed. IgG (1 ug/IP) was used as negative IP control. QPCR was performed with primers for a region approximately 1 kb upstream of the GAPDH and ACTB promoters, used as positive controls, and for the coding region of the inactive MYOD1 gene and the Sat2 satellite repeat, used as negative controls. Figure shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).](http://images.novusbio.com/fullsize/Histone-H2B-Antibody-Chromatin-Immunoprecipitation-NBP2-59271-img0003.jpg)
![ELISA: Histone H2B [ac Lys12] Antibody [NBP2-59271] - Histone H2B Antibody [NBP2-59271] - To determine the titer of the antibody, an ELISA was performed using a serial dilution of the antibody directed against H2BK12ac in antigen coated wells. The antigen used was a peptide containing the histone modification of interest. By plotting the absorbance against the antibody dilution, the titer of the antibody was estimated to be 1:38,200.](http://images.novusbio.com/fullsize/Histone-H2B-Antibody-ELISA-NBP2-59271-img0002.jpg)
![Immunofluorescence: Histone H2B [ac Lys12] Antibody [NBP2-59271] - Histone H2B Antibody [NBP2-59271] - HeLa cells were stained with the antibody against H2BK12ac and with DAPI. Cells were fixed with 4% formaldehyde for 10 minutes and blocked with PBS/TX-100 containing 5% normal goat serum and 1% BSA. The cells were immunofluorescently labeled with the H2BK12ac antibody (left) diluted 1:500 in blocking solution followed by an anti-rabbit antibody conjugated to Alexa Fluor 488. The middle panel shows staining of the nuclei with DAPI. A merge of the two stainings is shown on the right.](http://images.novusbio.com/fullsize/Histone-H2B-Antibody-Immunofluorescence-NBP2-59271-img0004.jpg)
![Western Blot: Histone H2B [ac Lys20] Antibody (PS01-46) [NBP3-32427] - Western blot analysis of Histone H2B on different lysates with Rabbit anti-Histone H2B antibody (NBP3-32427) at 1/1,000 dilution. Lane 1: Hela whole cell lysate Lane 2: Hela treated with 500 ng/ml TSA for 4 hours whole cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 14 kDa Observed band size: 14 kDa Exposure time: 2 minutes; 15% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32427) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32427_rabbit-histone-h2b-ac-lys20-mab-ps01-46-27820241631664.jpg "Western Blot: Histone H2B [ac Lys20] Antibody (PS01-46) [NBP3-32427] - Western blot analysis of Histone H2B on different lysates with Rabbit anti-Histone H2B antibody (NBP3-32427) at 1/1,000 dilution. Lane 1: Hela whole cell lysate Lane 2: Hela treated with 500 ng/ml TSA for 4 hours whole cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 14 kDa Observed band size: 14 kDa Exposure time: 2 minutes; 15% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32427) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: Histone H2B [ac Lys20] Antibody (PS01-46) [NBP3-32427] - Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue with Rabbit anti-Histone H2B antibody (NBP3-32427) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32427) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32427_rabbit-histone-h2b-ac-lys20-mab-ps01-46-298202414333645.jpg)
![Immunocytochemistry/ Immunofluorescence: Histone H2B [ac Lys20] Antibody (PS01-46) [NBP3-32427] - Immunocytochemistry analysis of Hela cells labeling Histone H2B with Rabbit anti-Histone H2B antibody (NBP3-32427) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-Histone H2B antibody (NBP3-32427) at 1/50 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.](http://images.novusbio.com/fullsize/nbp3-32427_rabbit-histone-h2b-ac-lys20-mab-ps01-46-2920241192354.jpg)
![Western Blot: Histone H2B [ubiquitylated Lys123] Antibody (C.15200232) [NBP2-59149] - Histone H2B Antibody (C.15200232) [NBP2-59149] - Analysis on whole cell extracts from WT yeast cells (lane 1), yeast cells with higher steady state levels of H2BK123ub (lane 2), none ubiquitinylated H2B (lane 3) and ubiquitinylated H2A (lane 4). The position of the protein of interest is indicated on the right.](http://images.novusbio.com/fullsize/Histone-H2B-Antibody-C-15200232-Western-Blot-NBP2-59149-img0002.jpg "Western Blot: Histone H2B [ubiquitylated Lys123] Antibody (C.15200232) [NBP2-59149] - Histone H2B Antibody (C.15200232) [NBP2-59149] - Analysis on whole cell extracts from WT yeast cells (lane 1), yeast cells with higher steady state levels of H2BK123ub (lane 2), none ubiquitinylated H2B (lane 3) and ubiquitinylated H2A (lane 4). The position of the protein of interest is indicated on the right.")
![Chromatin Immunoprecipitation: Histone H2B [ubiquitylated Lys123] Antibody (C.15200232) [NBP2-59149] - Histone H2B Antibody (C.15200232) [NBP2-59149] - ChIP assays were performed using human HeLa cells, the antibody consisting of 0.5, 1, 2 and 5 ug per ChIP experiment was analysed. IgG (1 ug/IP) was used as negative IP control. QPCR was performed with primers for the coding regions of the active CCT5 and EIF2S3 genes, used as positive controls, and for the inactive TSH2B gene and the Sat2 satellite repeat region used as negative controls. Figure shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).](http://images.novusbio.com/fullsize/Histone-H2B-Antibody-C-15200232-Chromatin-Immunoprecipitation-NBP2-59149-img0003.jpg)
![Western Blot: Histone H2B [ubiquitylated Lys123] Antibody (C.15200232) [NBP2-59149] - Histone H2B Antibody (C.15200232) [NBP2-59149] - Analysis on histone extracts from HeLa cells (15 ug) using the antibody against H2BK123ub. The antibody was diluted 1:500 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (kDa) is shown on the left.](http://images.novusbio.com/fullsize/Histone-H2B-Antibody-C-15200232-Western-Blot-NBP2-59149-img0001.jpg)
![Chromatin Immunoprecipitation: Histone H2B [ubiquitylated Lys123] Antibody (C.15200232) [NBP2-59149] - ChIP results obtained with the antibody against H2BK123ub on sheared chromatin from WT yeast cells, yeast cells with higher steady state levels of H2BK123ub and yeast cells with no ubiquitinylated H2B. Quantitative PCR was performed with primers for the coding region of an active gene. Figure shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).](http://images.novusbio.com/fullsize/Histone-H2B-Antibody-C-15200232-Chromatin-Immunoprecipitation-NBP2-59149-img0004.jpg)
![Western Blot: Histone H2B Antibody [NBP3-32426] - Western blot analysis of Histone H2B on different lysates with Rabbit anti-Histone H2B antibody (NBP3-32426) at 1/2,000 dilution. Lane 1: MCF-7 cell lysate (10 ug/Lane) Lane 2: Hela cell lysate (10 ug/Lane) Lane 3: A549 cell lysate (10 ug/Lane) Lane 4: 293T cell lysate (10 ug/Lane) Lane 5: THP-1 cell lysate (10 ug/Lane) Lane 6: K562 cell lysate (10 ug/Lane) Lane 7: HL-60 cell lysate (10 ug/Lane) Lane 8: NIH/3T3 cell lysate (10 ug/Lane) Lane 9: Zebrafish tissue lysate (20 ug/Lane) Lane 10: PC-12 cell lysate (10 ug/Lane) Lane 11: RAW264.7 cell lysate (10 ug/Lane) Predicted band size: 14 kDa Observed band size: 14 kDa Exposure time: 1 minute; 15% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32426) at 1/2,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32426_rabbit-histone-h2b-pab-278202416285267.jpg "Western Blot: Histone H2B Antibody [NBP3-32426] - Western blot analysis of Histone H2B on different lysates with Rabbit anti-Histone H2B antibody (NBP3-32426) at 1/2,000 dilution. Lane 1: MCF-7 cell lysate (10 ug/Lane) Lane 2: Hela cell lysate (10 ug/Lane) Lane 3: A549 cell lysate (10 ug/Lane) Lane 4: 293T cell lysate (10 ug/Lane) Lane 5: THP-1 cell lysate (10 ug/Lane) Lane 6: K562 cell lysate (10 ug/Lane) Lane 7: HL-60 cell lysate (10 ug/Lane) Lane 8: NIH/3T3 cell lysate (10 ug/Lane) Lane 9: Zebrafish tissue lysate (20 ug/Lane) Lane 10: PC-12 cell lysate (10 ug/Lane) Lane 11: RAW264.7 cell lysate (10 ug/Lane) Predicted band size: 14 kDa Observed band size: 14 kDa Exposure time: 1 minute; 15% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32426) at 1/2,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: Histone H2B Antibody [NBP3-32426] - Immunohistochemical analysis of paraffin-embedded rat large intestine tissue with Rabbit anti-Histone H2B antibody (NBP3-32426) at 1/3,400 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32426) at 1/3,400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32426_rabbit-histone-h2b-pab-29820241430413.jpg)
![Western Blot: Histone H2B [ac Lys20] Antibody (GT578) [NBP3-13538] - Untreated (-) and treated (+) HeLa whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with Histone H2B (acetyl Lys20) antibody [GT578] (NBP3-13538) diluted at 1:1000.](http://images.novusbio.com/fullsize/Histone-H2B-ac-Lys20-Antibody-GT578-Western-Blot-NBP3-13538-img0001.jpg "<b>Biological Strategies Validation. </b>Western Blot: Histone H2B [ac Lys20] Antibody (GT578) [NBP3-13538] - Untreated (-) and treated (+) HeLa whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with Histone H2B (acetyl Lys20) antibody [GT578] (NBP3-13538) diluted at 1:1000.")
![<b>Biological Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: Histone H2B [ac Lys20] Antibody (GT578) [NBP3-13538] - Histone H2B (acetyl Lys20) antibody [GT578] detects Histone H2B (acetyl Lys20) protein at nucleus by immunofluorescent analysis. Sample: Mock and treated HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Histone H2B (acetyl Lys20) stained by Histone H2B (acetyl Lys20) antibody [GT578] (NBP3-13538) diluted at 1:500. Red: phalloidin, a cytoskeleton marker, diluted at 1:100. Scale bar= 10 um.](http://images.novusbio.com/fullsize/Histone-H2B-ac-Lys20-Antibody-GT578-Immunocytochemistry-Immunofluorescence-NBP3-13538-img0002.jpg)
![Immunohistochemistry-Paraffin: Histone H2B [ac Lys20] Antibody (GT578) [NBP3-13538] - Histone H2B antibody [GT578] detects Histone H2B protein at nucleus by immunohistochemical analysis.Sample: Paraffin-embedded mouse liver.Histone H2B stained by Histone H2B antibody [GT578] (NBP3-13538) diluted at 1:200.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-13538_mouse-histone-h2b-ac-lys-20-mab-gt578-9102024205696.jpg)
![Western Blot: Recombinant Human Histone H2B Protein [NBP3-07913] - Lane 1: MWM; Lane 2: 1.0 ug Human Histone H2B Protein.](http://images.novusbio.com/fullsize/Recombinant-Human-Histone-H2B-Protein-Western-Blot-NBP3-07913-img0002.jpg "Western Blot: Recombinant Human Histone H2B Protein [NBP3-07913] - Lane 1: MWM; Lane 2: 1.0 ug Human Histone H2B Protein.")
![Western Blot: Recombinant Human Histone H2B Protein [NBP3-07913] - Lane 1: MWM; Lane 2: 100 ng Human Histone H2B Protein probed with Histone H2B pAb.](http://images.novusbio.com/fullsize/Recombinant-Human-Histone-H2B-Protein-Western-Blot-NBP3-07913-img0001.jpg)